Ermap (NM_013848) Mouse Untagged Clone

CAT#: MC219367

Ermap (untagged) - Mouse erythroblast membrane-associated protein (Ermap), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ermap
• Synonyms: AA409279; AI666418
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219367 representing NM_013848
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAAGCGGCTAAAGAAACATGTGGTGGCGTGGAAAATGTGTGTAATGCCACACTCACGGAAGATGT
  CAGTTCATATGGAGAGACCAAGTCCCTGTGGTTCCTGGCTTGTCGGCTGTCTCTTCACCATAGCTGTCTT
  CCAGCCGCCTGTTCAGGTGTTAGGGGATGCTGGCAAGGTCTACATAGCCCCTCTTAGGGACACAGCCAAC
  TTGCCCTGCCCTCTCTTCCTCTGGCCTAACATGGTACTCAGCGAGATGAGGTGGTATCGGCCCGGACACC
  TACCTCGCACCCAGGCTGTCCACGTGTTCCGGGACGGGCAGGACAGAGATGAAGACCTGATGCCAGAATA
  TAAGGGCAGGACGGCACTGGTGAGGGATGCCCACAAGGAAAGCTACATCCTGCAAATCAGTAATGTGAGG
  CTCGAGGACCGAGGGCTATACCAGTGCCAGGTCTGGGTCGGAAACTCAAGTCGAGAAGACAACGTGACCC
  TGCAGGTGGCAGTTTTAGGTTCTGATCCCTACATCCACGTGAAGGGCTATGATGCTGGGTGGATCGAGCT
  GCTGTGTCAATCAGTGGGATGGTTCCCAAAGCCGTGGACTGAGTGGAGAGACACTACGGGTAGAGCGCTA
  CTCTCCCTTTCAGAGGTTCACTCCCTGGATGAAAATGGGCTGTTCCGAACAGCAGTGTCCAGCAGAATCA
  GGGACAATGCCCTGGGAAATGTGTCTTGCACTATCCATAATGAGGCCCTTGGCCAAGAGAAGACGACGGC
  CATGATTATTGGAGCCCCAGAACGAGGAAGTCTGTCCTCTCCAGCGGTGGCTCTTTCTGTGGTCTTGCCC
  GTCTTGGGGCTCCTCATCCTCCTTGGCATCTGGCTCATTTGTAAACAAAAAAAATCAAAAGAGAAGCTGC
  TGTATGAGCAAGCGATGGAGGTAGAAAATCTTCTGGAAGACCACGCCAAAGAAAAAGGAAGACTCCACAA
  AGCCCTCAAGAAACTCCGGAGTGAACTGAAGTTGAAAAGAGCGGCGGCCAATGCAGGCTGGAGAAGAGCC
  CGGCTACATTTTGTGGCAGTGACCCTGGACCCTGACACGGCACATCCCAAACTCATCCTGTCTGAGGATC
  GGAGATGTGTGAGGCTCGGCGACAGGAAGCGGCCTGTTCCTGACAACCCAGAGAGGTTTGATTTTGTTGT
  CAGCGTCCTGGGCTCCGAGTATTTCACAACCGGCTGTCACTACTGGGAAGTGTACGTGGGCGAGAAGACC
  AAATGGATTCTTGGAGTGTGTAGTGAGTCTGTGAGCAGGAAAGGGAAGGTCACGGCCTCGCCTGCCAATG
  GCCACTGGCTTGTGCGACAGAGCCGTGGGAATGAGTATGAAGCTCTCACGTCCCCACAGACCTCCTTCCG
  CCTCAAAGAGTCCCCGAAGTGTGTGGGGATTTTCCTGGACTATGAAGCCGGCATCATCTCCTTCTACAAC
  GTGACTGATAAGTCCCACATCTTCACTTTCACCCACAGTTTCTCGAGCCCACTTCGCCCTTTCTTTGAAC
  CTTGTCTTCACGATGAGGGGAAAAACACGGCCCCTCTAATCATTTGTACTGAGCTTCAGAAATCAGAGGA
  ATCGATTGTCCCCAAACAAGAAGGAAAAGACCGTGCTAATGGAGACGTGTCCCTGAAGATGAACCCATCC
  TTACTGTCCCCTCAGGGTTCTGAGCTATTTCTGCTTAATGATACCTGGCCCTCTAACCTTGGCCCTGCCC
  TTAAGGGCCTCAAAGTTCCTTCTTTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013848
• Insert Size: 1779 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013848.2, NP_038876.2
• RefSeq Size: 4573 bp
• RefSeq ORF: 1779 bp
• Locus ID: 27028
• UniProt ID: Q9JLN5
• Cytogenetics: 4 55.34 cM
• Gene Summary: Possible role as a cell-adhesion or receptor molecule of erythroid cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.