Dnaaf3 (NM_001033548) Mouse Untagged Clone

CAT#: MC219361

Dnaaf3 (untagged) - Mouse RIKEN cDNA 6030429G01 gene (6030429G01Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dnaaf3
• Synonyms: 6030429G01Rik; b2b1739Clo
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219361 representing NM_001033548
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCACGCCTGCGGGCTCGGGCACCGGTTATGGTAGTGTGTCCTGGTGGGGTCTGTCCCCGGCGCTGG
  ACCTACAGGCAGAAAGTCCTCCTGTAGACCCAGATTCACAGTCGAAAACAGAGCGCAAGGTCCCAGAACT
  GGATGCCCTGCTGTTAGGTTCTGTGGATGGTAGACACATGCTGCGGACCCTGGCCAGGGCAGCGCTCTGG
  CCTCTCAGGAGCTTCAATTTTTATGTGCTGGAGAATAATCTGGAAGCGGTAGCCCGACACATGCTGATCT
  TCAGTCTAGCCCTAGAGGAACCTGAGAAGATGGGGCTCCAAGAGCGGAGTGAGACCTTCCTGGAGCTGTG
  GGGGAACGCGCTGCTGCGACCCTCGGTAGCTGCCTTCCTGCGCGCTCAGGCTAGCCACCTGGCTAATCTG
  GTCCTGGAGCCTGATCGCCTGGAGGAGCAACTACCCTGGCTTAGTCTTCGGCTGCTCAAGTTCCGCGAGC
  GGGATGCCCTGGAGGCCGTGTTCCGTTTCTGGTCTGGTGGGGAGAAGGGACCTGAAGTCTTTCCCATGAG
  TCGCCTTTGGGACTCGAGGTTGCGCCACTACCTGGGTTCCCGCTACGATGCCCGGCGTGGTGTTGCCGAC
  TGGGACCTACGCATGAAGCTGCATGATCGAGGGGCCCAAGTTATCCATATCCAGGAATTCCGACGCTGGC
  GGGATACAGGTGTTGCCTTTGAACTCAGAGACTTGAGTGCTTACCATGTGCCCAACAGGACTCTGGCATC
  AGGTCGCCTCCTGAGCCACCGTGGGGAACGCGTGGCTGCGCGCGGATACTGGGGGGACATCGCCACGGGG
  CCCTTTATGGCTTTTGGCATCGAGGCGGACGACCAAAGCCTCTTACGGACGAGAAACGGACAACCAGTCA
  AGACTGCGAGCGAGATCACGCAGCACAACGTCACAGAGCTGTTCCGAGAAGTAGCCGCCTGGAGGGGCCC
  GAGAGCCATCCAGGGGAATGTGGAGGAGACAGAGTCGCCCGAGCCAGATGTGCCTGCTCAAGAACCCTTC
  ACTATCCACTTTCTGCCTCTCGATTCTTCGCAGACTCTCCACCACAAGACTTGCTACCAGGGCCGGTTCC
  AGCTCCTCTACGTGTCCTGTGGGATGGTCCATCTTCTCAGCCCTGAGCTCGGGGCTTGCGTGGCCCCCGG
  AGGGAACCTGGTTGTGGAATTAGCTCGGTACTTGGTGGACCTGCGGCCCAAGGAGTTGAAGGCGTTCTCT
  GACCGCGTTGTAGAGATAGCGCGGGTGGCTGGGTTCGCCCCTCACACTGCAACCAAACCCTCGGAGACTT
  TTGCGAGGTTCTACAAGTTAGGGGACTCCACTCCGGGTGGCGGAGACTCAGCTGTGGAATCTGGACCCGC
  GCCCTCCAAAGTGGAATCTACCCGCGCCCCTCTCCCAGAATCGATAAGTCCGCCCCAGGCTAACCAGGCC
  CCCTCTCTAGAGGCGATGAGCCCACCCCTGGCTGACCTGGCCCCACCTCTAGAGACAATGTGTCCACCCC
  AGGCCAACCAGGCCCCTCCTCTAGAAGCGTTGAGCCCATCCAAAGCTGACCAGATCCCACCTCTAGAGGC
  GATGAGCCCACCCCAGGCTAAGCTAGTCCTGCCTGTAGAAGCAATCAGCCTTCCCCAAGCCGACCTGGCC
  TCTCCTCCGGAAGTGATAAGCCCACTCCAGGAAGCTATGGCAACGTCCTGGGTGAATGCACCTCCAAAAC
  ATGTAACATGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001033548
• Insert Size: 1761 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033548.2, NP_001028720.1
• RefSeq Size: 2189 bp
• RefSeq ORF: 1761 bp
• Locus ID: 436022
• UniProt ID: Q3UYV8
• Cytogenetics: 7 A1
• Gene Summary: Required for the assembly of axonemal inner and outer dynein arms. Involved in preassembly of dyneins into complexes before their transport into cilia (By similarity).[UniProtKB/Swiss-Prot Function]