Acsf3 (NM_144932) Mouse Untagged Clone

CAT#: MC219352

Acsf3 (untagged) - Mouse acyl-CoA synthetase family member 3 (Acsf3), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Acsf3
• Synonyms: BB101783
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219352 representing NM_144932
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCACCTCACTTGGCACTGCCCTTCAGGCGTCTCTTCTGGTCCTTGGCCTCCAGTCAGCTGATTCCCA
  GAAGACACCGAGGACATAGCCTCCTGCCTACCACCCCAGAGGCCCACACGGATGGGAGTGTCCCGGTATT
  CATCCGTGCCCTGGCCTTTGGGGACAGGATTGCCCTTATTGACAAATATGGCCATCACACCTACAGGGAA
  CTGTATGATCGCAGCCTTTGTCTGGCCCAGGAGATCTGCAGGCTTCAGGGTTGTAAAGTTGGGGACCTCC
  AGGAAGAAAGGGTCTCCTTCCTGTGCTCCAATGACGTCTCCTACGTTGTAGCACAGTGGGCCTCCTGGAT
  GAGTGGTGGTGTTGCTGTCCCACTGTACTGGAAGCACCCTGAGGCCCAGCTGGAGTATTTCATCCAGGAC
  TCCCGGAGCTCTTTGGTGGTGGTTGGCCAGGAGTACTTGGAGCGGCTAAGTCCACTGGCTCAGAGGCTGG
  GAGTCCCTCTTCTGCCGCTCACGCCTGCCGTCTACCATGGAGCAACAGAGAAGCCCACAGAGCAGCCAGT
  TGAAGAGAGTGGGTGGCGTGACCGGGGTGCCATGATCTTCTACACCAGCGGGACCACAGGGAGGCCCAAG
  GGTGCACTGAGCACCCACCGTAACCTAGCCGCTGTGGTGACTGGGCTGGTCCACTCATGGGCGTGGACTA
  AAAACGATGTGATCCTTCACGTCCTCCCGCTGCACCATGTCCACGGCGTGGTCAACAAGCTGCTCTGTCC
  ACTCTGGGTAGGAGCCACCTGTGTCATGCTGCCGGAGTTCAGTGCTCAGCAGGTTTGGGAAAAATTCTTG
  AGTTCTGAAGCCCCACAGATTACCGTATTCATGGCAGTGCCCACTGTCTACAGCAAGCTGCTGGACTACT
  ATGACAAGCATTTCACGCAGCCCCATGTCCAGGATTTTGTGCGTGCAGTTTGTAAAGAAAGAATCAGGCT
  GATGGTCTCAGGTTCGGCCGCTCTGCCTGTCCCACTGCTGGAGAAGTGGAGGAGCGCCACGGGCCACACG
  CTGCTGGAGCGCTATGGCATGACAGAGATCGGCATGGCTCTGTCCAACCCCCTGACGGAGGCTCGCGTGC
  CAGGTTCTGTGGGGACCCCATTGCCAGGAGTGGAAGTACGCATCATCTCAGAAAACCCGCAGAAGGGTTC
  CCCCTACATCATCCATGCAGAGGGAAACGAGAGGGGGACAAAGGTGACTCCAGGGTTCGAGGAAAAGGAA
  GGGGAGCTGCTGGTTAGGGGACCCTCGGTGTTCCGAGAATACTGGGATAAGCCAGAAGAAACGAAAAGTG
  CTTTCACTTCCGATGGCTGGTTCAGAACAGGAGACACCGCCGTGTTCAAGGATGCTAGGTACTGGATCCG
  AGGGCGCACGTCTGTGGACATCATCAAGACTGGAGGCTATAAAGTCAGCGCCCTGGAGATCGAGCGGCAC
  CTGCTGGCCCACCCCAGCATCACCGATGTCGCTGTAATTGGAGTTCCTGATATGACATGGGGCCAGCGGG
  TCACAGCTGTGGTTGCTCTTCAAGAAGGACATTCACTGTCCCACGGGGATCTCAAGGAGTGGGCCAGAGG
  TGTCCTGGCCCCCTATGCTGTGCCCTCGGAGCTCCTGCTGGTGGAGGAGATCCCCCGGAACCAGATGGGC
  AAGGTGAATAAGAAGGAGCTGCTCAAACAGTTGTACCCCTCAGGACAGAGGAGCCAGCCAGGCCAAGGCT
  AG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_144932
• Insert Size: 1752 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144932.3, NP_659181.2
• RefSeq Size: 2236 bp
• RefSeq ORF: 1752 bp
• Locus ID: 257633
• UniProt ID: Q3URE1
• Cytogenetics: 8 E1
• Gene Summary: Catalyzes the initial reaction in intramitochondrial fatty acid synthesis, by activating malonate and methylmalonate, but not acetate, into their respective CoA thioester. May have some preference toward very-long-chain substrates (By similarity).[UniProtKB/Swiss-Prot Function]