Trim45 (NM_001165952) Mouse Untagged Clone

CAT#: MC219344

Trim45 (untagged) - Mouse tripartite motif-containing 45 (Trim45), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim45
• Synonyms: 4921530N01Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219344 representing NM_001165952
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGAAATCAGGAAGCCGCTTCTGGGTTTCGTGCACAAACTCCAAGATGCGAACGCGTCTGGAAGCT
  CAGGCAAGACTCATTGTCCTACATGTCTGAGGCTTTTCAAAGTCCCCAGGCTCTTGCCTTGCTTGCACAC
  AGTGTGCACCACGTGTCTGGAAAAGCTGGATCCATTCTCAGTAGTGGACATCCGTGGGGGTGACTCAGAT
  ACAAGCTCTGAGGGGTCAGTATTCCAGGACCCCGAGTTATGTAGCCTGCAGCCACAGATCGGCATCCTCT
  GTCCGGTATGTGATGCTCAAGTGGACCTGCCCTTGGGTGGAGTGAAGGCTTTAACGGTAGACCACCTGGC
  CATGAATGATGTGCTGCTGGAGAACCTGCGTGGGGAAGGCCAGGGCCTGGTGTGTGACCTGTGCAGCGAC
  AGAGAAGTAGAGAAGAGGTGTCAGACCTGCAAAGCCAATCTCTGCCACTTCTGCTGCCAGGCTCATAGGC
  GACAGAAGAAGACGACATATCACACCATGGTGGACCTAAAAGACCTGAAAGGCTACAGCCAGGTTGGAAA
  GCCCATCCTATGTCCTTCTCACCCTGCAGAGGAGCTGAGGCTGTTCTGTGAACTCTGTGACCGGCCGGTG
  TGTCGGGACTGTGTAGTTGGGGAGCACCGAGAGCACCCCTATGACTTCACCAGCAATGTCATCCACAAGC
  ATGGAGATTCTGTGCGGGAGCTGCTTCGAGACACCCAGCCCCATGTGGAAGCCCTGGAAGATGCCCTGGC
  ACAGATTAAAAGTGTGAACAACGCCCTCCAAGAGCGAGTGGAGGCAGTGGCAGCTGACGTCCGGACATTC
  TCTGAGGGCTACATCAAAGCCATCGAGGAACACCGGGACAAGCTGCTGCAGCAGCTGGATGACATACGGA
  TCCAGAGGGAAACCGCTTTACAGCTGCAGAAAGCGCAGCTGGAACAGCTGCTGGCAGATATGCGGACTGG
  AGTGGAGTTCACCGAGCATCTGCTGACCAGCGGCTCGGACTTAGAGATTCTCATCACCAAGGGAGTGGTT
  GTAGAGCGGCTCAGGAAGCTGAATAAAGTTGAATACAGTGCCCGCCCAGGAGTGAACCATAAGATCTGCT
  TCTCCCCCCAGGAGAAGGCAGGCCAGTGCCAAGGCTATGAAGTGTATGGGGCCATTAACACGCAGGAAGT
  TGATCCAGCTCAGTGTGTCCTTCAGGGAGAAGATCTCCACAGAGCTCGAGAGAAACAGACAGCCTCTTTT
  ACCTTGTTCTGTAAGGATGCCTCGGGACAGAGCATGGGCAGGGGAGGAGATAATGTCCATGTGGAAGTTG
  TCCCCAAAGATAAGAAGGACAGTCCGATCAGAACAGTGGTCCAGGACAACAAGGATGGGTCGTACCGCGT
  TTCCTACACCCCCAAGGAGCCTGGCATCTACACCGTGTGGGTCTGCATCAGAGAGCAGCACGTGCAGGGC
  TCACCATTCAACGTGACCGTGAGGAGGAAGCATCGGCCACATCCGGGTGTGTTTCACTGCTGCACCTTCT
  GCTCCAGTGGAGGACAGAAAGCTGCCCGCTGTGCCTGCGGAGGCACCATGCCGGGTGGATACCTTGGCTG
  TGGCCACGGACACAAAGGCCACCCGGGCCGTCCACACTGGTCTTGCTGTGGGAAGTTCATTGAGAAGTCC
  GAGTGCTCGTTCACGAGTGGGCAGGGCGCCCCGAGGAGTCTGCTTAGGACCGTGGCGCTCTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001165952
• Insert Size: 1743 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001165952.1, NP_001159424.1
• RefSeq Size: 3342 bp
• RefSeq ORF: 1743 bp
• Locus ID: 229644
• UniProt ID: Q6PFY8
• Cytogenetics: 3 F2.2
• Gene Summary: May act as a transcriptional repressor in mitogen-activated protein kinase signaling pathway.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 3' UTR and lacks an alternate exon that results in a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is shorter than isoform a. Both variants 2 and 3 encode the same isoform.