Kat7 (NM_001195004) Mouse Untagged Clone

CAT#: MC219340

Kat7 (untagged) - Mouse MYST histone acetyltransferase 2 (Myst2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kat7
• Synonyms: Hbo1; Hboa; Myst2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219340 representing NM_001195004
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCGAAGGAAGAGAAATGCAGGCAGTAGTTCAGATGGAACCGAAGATTCCGATTTTTCTACAGATC
  TCGAGCATACAGACAGTTCAGAGAGTGATGGCACCTCGCGCCGATCTGCCCGAGTCACCCGCTCCTCAGC
  CAGGCTAAGCCAGAGTTCCCAAGATTCCAGCCCTGTTCGAAATTTGCCGTCTTTCGGCACTGAGGAGCCT
  GCCTATTCTACCAGAAGGGTGACCCGTAGTCAGCAGCAGCCCACGCCAGTGACTCCAAAAAAGTACCCTC
  TTCGACAGACTCGCTCATCTGGCTCTGAAACGGAGCAGGTGGTGGACTTTTCAGATAGAGAAACTAAAAA
  TACAGCTGATCATGATGAGTCACCACCTCGAACTCCAACCGGAAATGCACCTTCTTCTGAGTCTGACATA
  GACATCTCCAGCCCCAACGTGTCTCATGATGAGAGCATTGCCAAGGACATGTCCCTGAAGGACTCAGGCA
  GTGATCTCTCTCACCGTCCCAAGCGACGTCGATTTCACGAAAGCTACAATTTCAACATGAAGTGTCCTAC
  GCCGGGCTGCAACTCTCTAGGACATCTTACAGGAAAGCACGAGAGACATTTCTCCATCTCAGGATGCCCG
  CTGTATCATAACCTCTCAGCTGACGAATGCAAGGCACCAACAGAGAGACAGCTGCGGTATAAGGAAAAGG
  TGGCTGAACTCAGGAAGAAAAGAAATTCTGGACTGAGCAAAGAGCAGAAGGAGAAATACATGGAACACAG
  ACAGACCTATGGGAACACTCGGGAGCCTCTCTTGGAAAACCTGACAAGTGAATATGACTTAGATCTTTTC
  CGAAGAGCACAAGCCCGGGCTTCTGAGGATTTGGAGAAGTTAAGGCTTCAAGGCCAAATCACAGAGGGAA
  GCAACATGATTAAAACAATTGCTTTTGGCCGCTATGAACTGGACACTTGGTACCACTCTCCCTATCCTGA
  GGAATATGCGAGGCTGGGACGTCTCTACATGTGTGAATTCTGCTTAAAGTACATGAAGAGCCAGACAATC
  CTCCGCCGACACATGGCCAAGTGTGTGTGGAAACACCCCCCTGGTGATGAGATTTATCGCAAAGGCTCCA
  TCTCTGTGTTTGAAGTAGACGGCAAGAAGAACAAGATCTACTGCCAAAACCTCTGCCTTTTGGCAAAGCT
  TTTTCTGGACCATAAGACGCTATACTATGATGTAGAACCTTTCCTGTTCTACGTTATGACAGAAGCGGAC
  AATACTGGCTGTCATCTGATTGGATATTTTTCTAAGGAGAAGAATTCATTTCTCAACTATAATGTCTCGT
  GCATCCTCACCATGCCTCAGTACATGCGACAGGGCTACGGGAAGATGCTGATCGATTTCAGTTATTTACT
  TTCCAAAGTAGAAGAGAAAGTCGGCTCTCCAGAGCGCCCACTGTCAGACCTGGGGCTCATAAGCTACCGC
  AGCTACTGGAAGGAGGTACTGCTCCGATACCTGCACAACTTCCAGGGCAAGGAGATCTCCATCAAAGAAA
  TTAGCCAGGAAACAGCTGTGAATCCCGTGGACATTGTCAGCACTCTGCAAGCCCTTCAGATGCTCAAGTA
  TTGGAAAGGCAAGCACCTAGTTTTAAAGAGACAGGACCTGATAGATGAGTGGATAGCCAAAGAGGCCAAA
  AGGTCCAACTCCAACAAAACCATGGATCCAAGCTGCTTAAAATGGACCCCTCCCAAGGGCACTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001195004
• Insert Size: 1746 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001195004.1, NP_001181933.1
• RefSeq Size: 5876 bp
• RefSeq ORF: 1746 bp
• Locus ID: 217127
• UniProt ID: Q5SVQ0
• Cytogenetics: 11 D
• Gene Summary: Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Involved in H3K14 (histone H3 lysine 14) acetylation and cell proliferation (PubMed:23319590). Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Acts as a coactivator of the licensing factor CDT1. Specifically represses AR-mediated transcription.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.