Slc5a7 (NM_022025) Mouse Untagged Clone

CAT#: MC219302

Slc5a7 (untagged) - Mouse solute carrier family 5 (choline transporter), member 7 (Slc5a7), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slc5a7
• Synonyms: CHT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219302 representing NM_022025
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTTCCACGTAGAAGGACTGGTAGCTATTATCCTCTTCTACCTCCTTATATTTCTGGTTGGAATAT
  GGGCTGCATGGAAAACCAAAAACAGCGGCAACCCAGAAGAGCGCAGTGAAGCCATCATAGTCGGGGGCCG
  TGACATTGGTTTGTTGGTTGGTGGTTTTACCATGACAGCCACCTGGGTTGGAGGAGGCTACATCAATGGG
  ACAGCAGAAGCAGTGTATGGGCCAGGTTGTGGTCTAGCTTGGGCTCAGGCACCCATTGGATATTCTCTGA
  GTCTAATTTTAGGTGGTCTGTTTTTTGCGAAACCTATGCGTTCCAAGGGATATGTGACTATGTTAGACCC
  ATTTCAACAGATCTATGGAAAGCGCATGGGTGGGCTGCTCTTCATCCCTGCACTGATGGGAGAGATGTTC
  TGGGCTGCAGCAATTTTCTCTGCATTAGGGGCCACCATCAGCGTGATCATTGATGTGGATGTGAACATAT
  CGGTCATTGTCTCTGCACTCATTGCCATTCTTTATACCCTAGTGGGTGGGCTCTACTCTGTGGCATATAC
  TGATGTTGTCCAGCTATTCTGCATTTTTATAGGACTGTGGATCAGTGTCCCTTTTGCCCTGTCACATCCT
  GCAGTCACCGACATCGGATTCACAGCTGTGCATGCTAAATACCAGAGTCCCTGGCTGGGAACCATTGAAT
  CAGTTGAAGTCTACACCTGGCTTGATAATTTTCTGTTATTGATGCTGGGTGGAATCCCATGGCAAGCCTA
  CTTCCAGAGGGTCCTCTCTTCATCCTCAGCCACCTATGCTCAGGTACTGTCCTTCCTGGCAGCTTTTGGG
  TGCCTGGTGATGGCTCTACCCGCCATATGCATAGGAGCTATTGGAGCTTCCACAGACTGGAACCAGACTG
  CCTACGGGTATCCAGATCCCAAGACTAAGGAGGAAGCAGACATGATTCTCCCGATCGTTCTGCAGTACCT
  CTGCCCTGTGTACATCTCCTTCTTTGGGCTTGGTGCTGTTTCAGCTGCTGTCATGTCCTCAGCTGACTCG
  TCCATCCTGTCGGCGAGTTCTATGTTTGCTCGGAATATCTACCAGCTTTCCTTCAGACAAAATGCATCAG
  ACAAGGAAATTGTGTGGGTCATGAGGATCACTGTGCTTGTGTTCGGAGCATCTGCAACAGCCATGGCTTT
  GCTGACGAAGACTGTGTATGGGCTCTGGTACCTGAGCTCTGACCTTGTCTACATCATCATCTTCCCACAG
  CTGCTCTGTGTACTCTTCATCAAAGGAACCAACACTTATGGGGCAGTTGCTGGTTATATTTTTGGACTAT
  TCCTGAGAATTACTGGAGGAGAGCCATATCTATACTTGCAGCCCTTAATCTTCTACCCTGGTTATTACTC
  TGACAAGAATGGTATATACAATCAGAGGTTCCCATTTAAAACTCTCTCCATGGTTACCTCATTCTTTACC
  AACATTTGTGTTTCTTATCTAGCCAAGTATCTATTTGAAAGTGGAACCTTGCCTCCAAAATTAGATGTAT
  TTGATGCTGTTGTCGCAAGGCACAGTGAAGAGAACATGGACAAGACCATTCTAGTCAGAAATGAAAATAT
  CAAATTAAATGAACTTGCACCTGTGAAACCTCGGCAGAGCCTAACCCTCAGTTCAACTTTCACCAATAAG
  GAGGCCCTCCTTGATGTTGATTCCAGTCCGGAGGGGTCTGGGACTGAAGATAACTTACAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_022025
• Insert Size: 1743 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022025.4, NP_071308.2
• RefSeq Size: 5198 bp
• RefSeq ORF: 1743 bp
• Locus ID: 63993
• UniProt ID: Q8BGY9
• Cytogenetics: 17 C
• Gene Summary: Transmembrane transporter that imports choline from the extracellular space to the neuron with high affinity. Choline uptake is the rate-limiting step in acetylcholine synthesis. Sodium ion- and chloride ion-dependent.[UniProtKB/Swiss-Prot Function]