Map3k7 (NM_172688) Mouse Untagged Clone

CAT#: MC219291

Map3k7 (untagged) - Mouse mitogen-activated protein kinase kinase kinase 7 (Map3k7), transcript variant A, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Map3k7
• Synonyms: B430101B05; C87327; Tak1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219291 representing NM_172688
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGACAGCCTCCGCCGCCTCGTCCTCCTCCTCGTCTTCTGCCAGTGAGATGATCGAAGCGCCGTCGC
  AGGTCCTGAACTTCGAAGAGATCGACTACAAGGAGATCGAGGTGGAAGAGGTTGTCGGAAGAGGAGCTTT
  TGGAGTAGTTTGCAAAGCTAAGTGGAGAGCAAAAGATGTCGCTATTAAACAGATAGAAAGTGAGTCTGAG
  AGGAAGGCTTTCATTGTGGAGCTCCGGCAGTTGTCACGTGTGAACCATCCTAACATTGTCAAGTTGTATG
  GAGCCTGCCTGAATCCAGTATGTCTTGTGATGGAATATGCAGAGGGGGGCTCATTGTATAATGTGCTGCA
  TGGTGCTGAACCATTGCCTTACTACACTGCTGCTCATGCCATGAGCTGGTGTTTACAGTGTTCCCAAGGA
  GTGGCTTACCTGCACAGCATGCAGCCCAAAGCGCTGATTCACAGGGACCTCAAGCCTCCAAACTTGCTGC
  TGGTTGCAGGAGGGACAGTTCTAAAAATCTGCGATTTTGGTACAGCTTGTGACATCCAAACACACATGAC
  CAATAATAAAGGGAGTGCTGCTTGGATGGCGCCTGAAGTATTTGAAGGTAGCAATTACAGTGAAAAGTGT
  GATGTCTTCAGCTGGGGTATTATCCTCTGGGAAGTGATAACACGCCGGAAACCCTTCGATGAGATCGGTG
  GCCCAGCTTTCAGAATCATGTGGGCTGTTCATAATGGCACTCGACCACCACTGATCAAAAATTTACCTAA
  GCCCATTGAGAGCTTGATGACACGCTGTTGGTCTAAGGACCCATCTCAGCGCCCTTCAATGGAGGAAATT
  GTGAAAATAATGACTCACTTGATGCGGTACTTCCCAGGAGCGGATGAGCCGTTACAGTATCCTTGTCAGT
  ACTCTGATGAAGGGCAGAGCAACTCAGCCACCAGCACAGGCTCATTCATGGACATTGCTTCTACAAATAC
  CAGTAATAAAAGTGACACAAATATGGAACAGGTTCCTGCCACAAACGACACTATTAAACGCTTGGAGTCA
  AAACTTTTGAAAAACCAGGCAAAGCAACAGAGTGAATCTGGACGCCTGAGCTTGGGAGCCTCTCGTGGGA
  GCAGTGTGGAGAGCTTGCCCCCCACTTCCGAGGGCAAGAGGATGAGTGCTGACATGTCTGAAATAGAAGC
  CAGGATCGTGGCGACTGCAGGTAACGGGCAACCAAGGCGTAGATCCATCCAAGACTTGACTGTTACTGGG
  ACAGAACCTGGTCAGGTGAGCAGCCGGTCATCCAGCCCTAGTGTCAGAATGATCACTACCTCAGGACCAA
  CCTCAGAGAAGCCAGCTCGCAGTCACCCGTGGACCCCTGATGATTCCACAGATACCAATGGCTCAGATAA
  CTCCATCCCAATGGCGTATCTTACACTGGATCACCAGCTACAGCCTCTAGCGCCGTGCCCAAACTCCAAA
  GAATCCATGGCAGTGTTCGAACAACATTGTAAAATGGCACAGGAGTATATGAAAGTTCAAACCGAAATCG
  CATTGTTACTACAGAGAAAGCAAGAACTAGTTGCAGAATTGGACCAGGATGAAAAGGACCAGCAAAATAC
  ATCTCGTCTGGTACAGGAACATAAAAAGCTTTTAGATGAAAACAAAAGCCTTTCTACTTATTACCAGCAA
  TGCAAAAAACAACTAGAGGTCATCAGAAGCCAACAGCAGAAACGACAAGGCACTTCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172688
• Insert Size: 1740 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172688.3, NP_766276.1
• RefSeq Size: 5682 bp
• RefSeq ORF: 1740 bp
• Locus ID: 26409
• UniProt ID: Q62073
• Cytogenetics: 4 A5
• Gene Summary: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR) (PubMed:10748100, PubMed:16157589, PubMed:21183079, PubMed:29291351). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs, c-jun N-terminal kinases (JNKs) and I-kappa-B kinase complex (IKK). Both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1), while nuclear factor-kappa B is activated by IKK (PubMed:16157589, PubMed:8533096, PubMed:29291351). MAP3K7 activates also IKBKB and MAPK8/JNK1 in response to TRAF6 signaling and mediates BMP2-induced apoptosis (PubMed:10748100). In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK1, but not that of NF-kappa-B. Promotes TRIM5 capsid-specific restriction activity (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (A) lacks an in-frame coding segment, compared to variant B. The resulting isoform (A) lacks an internal region, as compared to isoform B. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.