Taf1b (NM_020614) Mouse Untagged Clone

CAT#: MC219283

Taf1b (untagged) - Mouse TATA box binding protein (Tbp)-associated factor, RNA polymerase I, B (Taf1b), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Taf1b
• Synonyms: 4930408G01Rik; A230108M10Rik; p63; TAFI68; Tafi86
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219283 representing NM_020614
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGTGGAGGAGGTGAAAGCGTTCAGAGACCGCTGTTCTCAGTGTGCTGCTGTCTCCTGGGGCCTTA
  CCGATGAAGGCAAATATTACTGTACTTCCTGCCACAATGTCACAGATCGATCTGAGGAAGTGGTGAGCGC
  TGCAGATATCCCCAACACCAAGATCAACTCCATCAACAGGGGGCTGAGACAGAGAAGCAAGCATGAGAAA
  GGCTGGGATTGGTATGTGTGTGAAGGTTTCCAGTGCATACTTTATCACCAAGCAAAAGCCCTGGAGACCC
  TCGGAGTAAGCCCAGAACTAAAGAATGAAGTCTTACACAATTTTTGGAAGCGCTACCTTCAGAAGAGCAA
  ACAGGCCTATTGTAAAAACCCAGTTCGTACAAGTGGAAGGAAAGCCAAGGTATTAGAAGACAGTGTGCAG
  TCCTCGGACTTGGGCAGTGATCTTGAGCTGCTAAGTGATACCACATGCCCCCTTGAAAGTGAAGCAGAGT
  TCCAATCTGACCCTCAGATTCCAAAGCCTTTCCCTGTCACCAAAGGATCCCCAAAGTCAGCGTCTGTCTG
  CTCTGGCTCTGTTGATGGAGTGGAGTACTCCGAGCGGAAGGAGAAGGGGCTTGTGAAGATGACGGTGCCC
  AGGACACTTGCTCTCTGCTCTCTCTCCTTGCTCTGGCAGAGGGAAACAATTACAGTTTCAGACCTTTTGA
  GGTTTGTTGAAGAGGATCATATTCCGTACATAAATGCTTTTAAGCTTTTTCCAGAAGAGATGAAAGTGTA
  TGGTCGTGACAAAGGCATCTTTGCTATAGAGTCTTGGCCTGACTATGAAGACATCTATAAAAACATGATT
  GAACTTGCCATATTTTTAGATTTGCCTCGTTTCCCAGACATAACGGAAGACTGCTACCTCCATCCCAACA
  CCTTGTGTATGAAATACTTACTGGAGGTCAATCTCCCTGATGAAATGCACACTTTAACCTGTCAAGTAGT
  AAAGTTGACTGGAATTGGGGAAGTGGATTTTCTGACGTTTGATCCTATAGCTAAAACGAAAAGGACAGTT
  AAATATGATGTGCAGGCTATGGCTGTCATTGTGGTTGTGTTGAAGCTGCTGTTTTTGCTGGATGACAAGC
  TGGAATGGTCTTACTCAGATCTTGCTGAAGCGTACAACGAACAACACAGAGAAGACACGCCCCAGTTTGA
  TTTCAGAAAATGGTACCAAGTTATGAAGAAAACTTTTGATGAGAAAAGACGAAAATGGGAAGAAGCGAGG
  GCCAGGTATGCTTGGAAAACCAAAAGGCCACTCTACCGTTCACACATCGATAAGTCCGTCGCATATAAGA
  GAAGAAAAATGGTGGAGAATCTTCAAAAACAATTTAGTGCCCTGGTTGGTTCATCACCCGTGGTTGAAAA
  GCAGGCCCCTTCAAGTTTTCAGTTCAACTGGACTGAAGAAGGCACTGATAGCCCTTGTTTCCATGGACAC
  AGCCTCCAGGGACTCCTGATAATGAAAGGCCAGTCCATGATAACCAAGAACTCCCTGTATTGGCTCAGTA
  CCCAGAAGTTCTGCAAAAGCTATTGTAAACATGTGACAACTTACGAAGAATCAAATTTTTCTTTGAGTTA
  CCAGTTTATACTAAACATCTTCTCCTTCTTACTGAGAATAAAGACCTCTGCTCTCCATGAAGAAGTGAGC
  TTACTTGAAAAGAAACTTTTTGAAAAAAAATACAACGAATCAAAAAAGAGCTCAGGATCCAAGAAAGGAA
  GAAGACACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_020614
• Insert Size: 1761 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020614.2, NP_065639.2
• RefSeq Size: 2019 bp
• RefSeq ORF: 1761 bp
• Locus ID: 21340
• UniProt ID: P97358
• Cytogenetics: 12 A1.3
• Gene Summary: Component of RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during transcription initiation such as pre-initiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. Binds rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex and, possibly, directly through its interaction with RRN3.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein.