Carm1 (NM_153141) Mouse Untagged Clone

CAT#: MC219264

Carm1 (untagged) - Mouse coactivator-associated arginine methyltransferase 1 (Carm1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Carm1
• Synonyms: Prmt4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219264 representing NM_153141
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGGCGGCAGCGACGGCGGTGGGGCCGGGTGCGGGGAGCGCTGGGGTGGCGGGCCCGGGCGGCG
  CGGGGCCCTGCGCTACAGTGTCTGTGTTCCCGGGCGCCCGCCTCCTCACTATCGGCGACGCGAACGGCGA
  GATCCAGCGGCACGCGGAGCAGCAGGCGCTGCGCCTTGAGGTGCGCGCCGGACCAGACGCGGCGGGCATC
  GCCCTCTACAGCCATGAAGATGTGTGTGTTTTCAAGTGCTCGGTGTCCCGAGAGACAGAGTGCAGTCGTG
  TGGGCAGACAGTCCTTCATCATCACCCTGGGCTGCAACAGCGTCCTCATCCAGTTTGCCACACCCCACGA
  TTTCTGTTCTTTCTACAACATCCTGAAAACCTGTCGGGGCCACACACTGGAGCGCTCTGTGTTCAGTGAG
  CGGACAGAGGAATCCTCAGCTGTGCAGTACTTCCAGTTCTATGGCTACCTATCCCAGCAGCAGAACATGA
  TGCAGGACTATGTGCGGACAGGCACCTACCAGCGTGCGATCCTGCAGAACCACACGGACTTCAAGGACAA
  GATCGTTCTAGATGTGGGCTGTGGCTCTGGGATCCTGTCATTTTTTGCTGCTCAAGCAGGAGCCAGGAAA
  ATTTATGCAGTGGAAGCCAGCACCATGGCTCAGCATGCAGAGGTCCTGGTGAAGAGTAACAATCTGACAG
  ACCGCATCGTGGTCATCCCTGGCAAAGTAGAGGAGGTCTCATTGCCTGAGCAAGTGGACATTATCATCTC
  AGAGCCCATGGGCTACATGCTCTTCAATGAACGAATGCTCGAGAGCTACCTCCATGCCAAAAAGTACCTG
  AAGCCTAGTGGAAACATGTTCCCCACCATTGGTGATGTCCACCTCGCACCCTTCACTGATGAACAGCTCT
  ACATGGAGCAGTTCACCAAAGCCAACTTCTGGTACCAGCCATCCTTCCATGGAGTGGACCTGTCGGCCCT
  CAGAGGCGCCGCTGTGGATGAGTACTTCCGGCAACCTGTGGTGGACACATTTGACATCCGGATCCTGATG
  GCCAAATCTGTCAAGTACACAGTGAACTTCTTAGAAGCCAAAGAAGGCGATTTGCACAGGATAGAAATCC
  CATTCAAATTCCACATGCTGCATTCAGGGCTAGTCCATGGCTTGGCCTTCTGGTTCGATGTTGCTTTCAT
  TGGCTCCATAATGACCGTGTGGCTATCCACAGCCCCAACAGAGCCCCTGACCCACTGGTACCAGGTCCGG
  TGCCTCTTCCAGTCACCGTTGTTTGCCAAGGCCGGGGACACGCTCTCAGGGACATGTCTGCTTATTGCCA
  ACAAAAGACAGAGCTATGACATCAGTATTGTGGCACAGGTGGACCAGACAGGCTCCAAGTCCAGTAACCT
  GCTGGATCTAAAGAACCCCTTCTTCAGGTACACAGGTACAACCCCATCACCCCCACCTGGCTCACACTAC
  ACGTCTCCCTCGGAGAATATGTGGAACACAGGAAGCACCTATAATCTCAGCAGCGGGGTGGCTGTGGCTG
  GAATGCCTACTGCCTACGACCTGAGCAGTGTTATTGCCGGCGGCTCCAGTGTGGGTCACAACAACCTGAT
  TCCCTTAGGCTCCTCAGGTGCCCAGGGAGGCGGCGGTAGCTCCAGTGCCCACTATGCAGTCAACAACCAG
  TTCACCATGGGTGGCCCTGCCATCTCTATGGCCTCGCCCATGTCCATCCCGACCAACACCATGCACTATG
  GGAGTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153141
• Insert Size: 1758 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153141.1, NP_694781.1
• RefSeq Size: 3151 bp
• RefSeq ORF: 1758 bp
• Locus ID: 59035
• UniProt ID: Q9WVG6
• Cytogenetics: 9 A3
• Gene Summary: Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an exon in the 3' coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.