Gpc3 (NM_016697) Mouse Untagged Clone

CAT#: MC219262

Gpc3 (untagged) - Mouse glypican 3 (Gpc3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gpc3
• Synonyms: OCI-5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219262 representing NM_016697
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGGACCGTGCGCACCGCGTGCTTGCTGGTGGCGATGCTGCTAGGCTTGGGCTGCCTGGGACAGG
  CGCAGCCCCCGCCGCCTCCAGACGCCACCTGTCACCAGGTCCGTTCTTTCTTCCAGAGACTGCAGCCCGG
  ACTCAAATGGGTTCCAGAAACCCCTGTACCAGGATCAGATTTGCAAGTATGTCTCCCCAAGGGCCCAACA
  TGCTGCTCAAGAAAGATGGAAGAAAAATACCAACTAACAGCACGGCTGAACATGGAACAACTGCTCCAGT
  CTGCGAGTATGGAACTCAAGTTCTTAATTATTCAGAATGCTGCGGTTTTCCAAGAGGCCTTTGAAATTGT
  TGTTCGCCATGCCAAGAACTACACCAACGCCATGTTCAAGAATAACTACCCCAGCCTGACTCCACAAGCT
  TTTGAGTTTGTCGGTGAATTTTTCACAGATGTGTCTCTCTACATCTTGGGTTCTGATATCAACGTGGATG
  ATATGGTCAATGAATTGTTCGACAGCCTCTTTCCAGTCATCTACACCCAGATGATGAACCCAGGCCTGCC
  TGAGTCAGTCTTAGACATCAACGAGTGCCTCCGAGGAGCAAGACGTGACCTGAAAGTATTTGGCAGTTTC
  CCCAAGCTTATTATGACCCAGGTTTCCAAGTCACTGCAAGTCACTCGAATCTTCCTTCAAGCCCTGAATC
  TCGGAATTGAAGTCATCAACACTACCGACCACCTCAAGTTTAGTAAGGACTGTGGCCGTATGCTCACCCG
  AATGTGGTATTGCTCTTACTGCCAGGGACTGATGATGGTTAAGCCTTGCGGTGGTTATTGCAATGTGGTC
  ATGCAAGGCTGTATGGCTGGTGTGGTGGAGATCGACAAGTACTGGAGAGAATACATTCTGTCTCTTGAAG
  AGCTCGTGAATGGCATGTACAGAATCTACGACATGGAGAATGTGCTGCTCGGCCTCTTTTCTACCATCCA
  TGATTCCATCCAGTATGTGCAGAAGAACGGAGGCAAGCTGACCACCACCATTGGCAAGTTGTGTGCCCAC
  TCCCAGCAACGCCAATATAGATCTGCTTATTACCCTGAAGATCTGTTTATTGACAAGAAGATATTAAAAG
  TCGCTCATGTCGAACATGAAGAAACCTTATCCAGCCGAAGAAGGGAACTGATTCAGAAACTGAAGTCTTT
  CATCAACTTCTATAGCGCTTTGCCGGGCTACATCTGCAGCCATAGCCCCGTGGCCGAAAATGATACCCTG
  TGCTGGAACGGACAAGAACTTGTGGAGAGATACAGCCAGAAGGCGGCAAGGAACGGGATGAAGAATCAGT
  TTAACCTCCATGAGCTGAAAATGAAGGGCCCTGAGCCGGTGGTTAGCCAGATCATTGACAAACTGAAGCA
  CATTAACCAGCTCCTGAGAACCATGTCTGTGCCCAAGGGTAAAGTTCTGGATAAAAGCCTGGATGAAGAA
  GGACTTGAAAGTGGAGACTGCGGTGATGATGAAGATGAATGCATTGGAAGCTCTGGTGACGGGATGGTGA
  AAGTGAAGAATCAACTGCGCTTCCTTGCAGAACTGGCCTATGATCTGGATGTGGACGATGCTCCGGGGAA
  CAAGCAGCATGGAAATCAGAAGGACAACGAGATCACCACCTCTCACAGCGTGGGGAACATGCCGTCCCCA
  CTGAAGATCCTCATCAGTGTGGCCATCTATGTGGCGTGCTTTTTTTTCCTGGTGCACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_016697
• Insert Size: 1740 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC036126, AAH36126
• RefSeq Size: 2312 bp
• RefSeq ORF: 1740 bp
• Locus ID: 14734
• UniProt ID: Q8CFZ4
• Cytogenetics: X A5
• Gene Summary: Cell surface proteoglycan that bears heparan sulfate (By similarity). Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins (PubMed:18477453, PubMed:23665349). Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation (PubMed:18477453). Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands (By similarity). Positively regulates the non-canonical Wnt signaling pathway (PubMed:15537637). Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (PubMed:23665349). Inhibits the dipeptidyl peptidase activity of DPP4 (By similarity). Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4 (PubMed:10964473). Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis (PubMed:11180950). Required for coronary vascular development (PubMed:19733558). Plays a role in regulating cell movements during gastrulation (By similarity).[UniProtKB/Swiss-Prot Function]