Fam83d (NM_027975) Mouse Untagged Clone

CAT#: MC219255

Fam83d (untagged) - Mouse family with sequence similarity 83, member D (Fam83d), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fam83d
• Synonyms: 2310007D09Rik; BB104611
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219255 representing NM_027975
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCTCGCTTCGAGCTGTTGGACGACCTTCCCGCGGCCTGCCTGTCTCCGTGCGGACCGCCCAACC
  CCACGGAGCTGTTCAGCGAGGCGCGGCGTCTGGCTCTCGAGCAGCTGCTGGCCGGTGGTCCCGACGCCTG
  GGCCGCCTTCTTACGGCGTGAGCGGCTGGGCCGCTTCCTCAACGCCGACGAGGTGCGCGAGGTTCTGGGC
  GCTGCCGAGCGGCCGGGCGAGGATGGGGCGGCGGTAGCGGAGGACTCGTTCGGCTCCTCTCACGAATGCT
  CGTCGGGCACCTACTTTCCGGAGCAGTCGGACCTGGAACCGCCGGCGTTGGAGCTCGGCTGGCCTTCCTT
  CTACCAGGGTGCCTACCGCGGTGCCACGCGTGTCGAGGCTCATTTCCAGCCCCGCGGCGCGGGCGCCGGA
  GGCCCGTATGGCTGCAAGGATGCGCTGCGCCAGCAGTTGCGCTCCGCCCGAGAGGTGATTGCTGTGGTAA
  TGGACGTTTTCTCAGACATCGACATCTTCCGAGACCTGCAGGAGAGCTGTAGGAAGCGGGGGGTGGCTGT
  GTACATCCTCCTGGACCAGACTCTGCTCCCCCACTTTTTGGACATGTGCATGGATCTGAGAGTTCATCCT
  GAGCAGGAAAAGCTGATGACAGTTCGGACAATTACAGGAAATATCTACTATGCAAGGTCAGGAACAAAGG
  TTGTCGGGAAGGTTCATGAGAAGTTCACACTGATTGACGGAATCCGGGTGGCAACAGGCTCTTACAGTTT
  TACCTGGACCGACGGCAAATTAAACAGCAGTAACTTGGTAATTCTGTCTGGCCAAGTGGTTGAACACTTT
  GATCTGGAGTTCCGGATCCTGTATGCTCAGTCAGAGCCCATCAGCTCCAAACTCCTGTCCAACTTCCAGA
  TCAATAGCAAGTTTGACCATCTGGCTGACCGAAAGCCACAGTCGAAGGAGCCCACACTGGGCAATCTGCT
  GCGAATGAGGCTGGCCAGGCTCTCAAGTACTCCCAGGAAGAGCAACCTGGGCCCAGAGGAGCCGCCAAAA
  GACAGAGCCAAACCCAAGCGCCCTGACTCTGAGGCTTCTACCATCAGCGATGAAGACTATTTCCACAGCC
  ACAAGGACCAGCTAGAGGATAGTAAGGTGGCTGATGCTGCTACCCAAACAGAGCCCAGAGAAGAGATGGC
  TGCAGTAAGCCTGAGTGAGGTGGGAACTCAGACTAGTTCCAGCATGATGTGTGTTGGGACCCAAACCACA
  GTTGTCACCAGGGCAGCGAGCTCCCAGGCCACAGTGTGGTCCAAGTCCACTACCACACAGACTGAAGCTG
  ACGAGAGCTTCCTTCCTCAGGGTGCCCAGTCCAAAGAAGGGTCACCGGCATCCAAGATGTCAGTGTCGAG
  GTCTTCCAGTGTGAGGTCATCCTCTTCTGTGTCTTCCCAGGGCTCGCTGGCAAGCTCGGTGAGCTCCCAT
  GTCTCCCTGACGGCTGCTGATCTCCACACTCCCGCATACCCCAAGTACCTGGGTCTGGGCACCCCGCATC
  TGGATCTGTGCCTAAGGGACTCGTTCAGAAACTTAAGTAAGGAGCGGCAGGTCCACTTCACTGGCATCAG
  GTCCCGGCTCACCCAGATGCTCACCGTGCTCTCACGGAGAACGCTCTTCACAGAGCACTACCTCAGCTAC
  AGCCCCGGGAGCTTTACCAGAGCCTCTACCAACCTGGTTTCTGTGAGGGACATAGCCCTTTACCCTCCCT
  ATCAGTGA
  
  ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
  TTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-NotI
• ACCN: NM_027975
• Insert Size: 1758 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027975.2, NP_082251.2
• RefSeq Size: 2280 bp
• RefSeq ORF: 1758 bp
• Locus ID: 71878
• UniProt ID: Q9D7I8
• Cytogenetics: 2 H1
• Gene Summary: Probable proto-oncogene that regulates cell proliferation, growth, migration and epithelial to mesenchymal transition. Through the degradation of FBXW7, may act indirectly on the expression and downstream signaling of MTOR, JUN and MYC. May play also a role in cell proliferation through activation of the ERK1/ERK2 signaling cascade. May also be important for proper chromosome congression and alignment during mitosis through its interaction with KIF22.[UniProtKB/Swiss-Prot Function]