Eme1 (NM_177752) Mouse Untagged Clone

CAT#: MC219234

Eme1 (untagged) - Mouse essential meiotic endonuclease 1 homolog 1 (S. pombe) (Eme1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eme1
• Synonyms: 6820428D13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219234 representing NM_177752
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTAAGAAGGTTATCCCTGTCTCGCCTCTCCACGGAGAGTGATTCTGAGGACCTGCCCACATTTG
  CCTTTTTGAAGAAGGAACCATCTTCAACCAACAGGAAGCCACCTCAGAGGGCGAAGAACATAGTGGTTGT
  CACCTCAGATTCTGAAGCCTCCTGTCCTCCATCACCAGGCCTCAAAGGTCCACCATGTGTCCCCAGTGCA
  GCTGGAGCTCCCCCACAAGCAGGGCCAGTCAGAGTGCTAAGCAGTAGCAGTGAGGACGAAGATGTATTTG
  TCCCCCTAGCTGAGAGGATCACATGTAAGCTTTTGACGAGCAAGCAGCTGTGCCCTGAGCTCTCTAGCTC
  CTCACTTAAAACAGGTTTGGATGGCCAAAATAATGCCAGCGCACCATGTGACTGGAAAAGGCAACCATGG
  CCAAAGATTCCCGATGTTCCCCTCCACGGTGCCTTAGAGAAGAGTGCTGCAAATGATGAGGACTCTCTCT
  TAGACGATCAGTGTCGTCAGCTTCCAACCTACCAGGCTACCTGCAGGGAGCTGGCAGTCTCCAAAACAAA
  TTCCGACCGACCTCTACCCAAGAAGAGAACCAAGCATATTCAGACGGTCCAGAGCGGAGGCTCTCAGGGA
  TGCTGGCGACCGGGACAGGCAAGCAGGAAGGAAAACACCCCGAGGCAGCATGAAAGAAAAAAGAAGGCAG
  AGATGATCAAGAGGCTCAAAGCCCAGAGGCCAGAGGAATGCCTGAAGCACATCGTGGTGGTGCTGGATCC
  AGTGCTTTTACAGATGGAAGGTGGGGGCCAGCTCCTGGGAGCGCTGCAGGCCATGGAGTGCAGCTGTGTG
  ATTGAAGTCCAGGCCATACCTCGAAGCATCACTTGGAGGAGGAGGAGGACAGAGCTGGTAGAGGATGGAG
  ATGACTGGATGGAAGAGCCAACAATCCTGGTGTTGGTCCTGGCAGAGGTCTTCATGTCCATGGCCTACAA
  CTTAAAGCAGGCAAGTCCTAGCAGCACCGAGAAAGGGAAGGAGACCCTTCGGAGCTTTGTAACTGATGTC
  ACAGCAAAGACGGGGAAAGCATTGTCACTGGTGATTGTGGACCAGGAGAAATGCTTCAGGCCTCAGAATC
  CCCCGAGGAGAAGGAAATCAGGAATGGCAAATAAACAGGCCAAAGCGAAGCATCAGCAGAGGCAAGAGTC
  TAGCACGGGACTCATGGTGTCCAGGGCAGACATGGAGAAGGCACTGGTGGATCTGCAGCTTTATACAGAA
  GCCCAGGCGTGGATGGTGCAGAGCTGGAAGGAGCTGGCGGACTTCACCTGTGCATTTACAAAGGCTGTGG
  CTGAAGCACCCTTCAAGAAGCTCCGGGATCAAGTCACCTTTTCCTTCTTCCTGGAGAAAGACTGGGCTGG
  AGGGATGAAAGTGGACCAGTCCGGCAGGGGACTGGCACTGATCTGGAGGCGGCAGATCCAGCAGCTGAAC
  CGAGTCAGCTCGGAGATGGCCAGTGCTATTGTGGACGCCTATCCCTCACCACAGCTCCTGGTGCAGGCTT
  ATCAGCGGTGTTTCTCTGAGCAAGAACGTCAGAATTTGCTGGCTGACATACAAGTGCGACGTGGGGAAGG
  TGTGACAGCCACCTCCCGCCGTGTTGGGCCAGAATTATCCAGGCGGATCTACCTTCAAATGACCACAGCG
  CAACCAGATCTCATCTTAGACAGTGTTGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177752
• Insert Size: 1713 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177752.4, NP_808420.1
• RefSeq Size: 2228 bp
• RefSeq ORF: 1713 bp
• Locus ID: 268465
• UniProt ID: Q8BJW7
• Cytogenetics: 11 D
• Gene Summary: Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.[UniProtKB/Swiss-Prot Function]