Galnt12 (NM_172693) Mouse Untagged Clone

CAT#: MC219224

Galnt12 (untagged) - Mouse UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 12 (Galnt12), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Galnt12
• Synonyms: 9130206E10; A630062B03Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219224 representing NM_172693
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGGGCGTGCGGTGAGGAGGCGCTGCCCGCGGGGGCTGCGGCGCGGCCGGGAGGCGTTGCTGGCAC
  TGCTGGCCCTGGCAGGGCTGGGCGCGCTGCTGCGGGCTCGAAGCAGGTCGGGGACGGTGGACCCGGGACC
  CCCGCGGACTCCTCTCCCCGGGCGTCACGAGCCAGTGCTGCCCAGGCCGCCGCTGCCCGCCGATGCTCTG
  GGAGCGCATGGGGAGGCGGTGCGGCTGCAGCTGCAGGGCGAGGAGCTGCGGCTGCAGGAGGAGAGCGTGA
  AGCAGCACCAGATCAACATCTATCTGAGCGACCGTATCTCGCTGCACCGCCGACTGCCCGAGCGCTGGAA
  CCCTCTGTGCAGAGAGGTGAAATACGATTATGATAACCTGCCCAAGACTTCTGTTGTCATAGCATTTTAT
  AATGAAGCCTGGTCCACACTCCTTCGGACAGTTTACAGTGTTCTTGAGACCTCCCCTGACATCCTGCTGG
  AGGAGGTCATTCTGGTAGATGACTACAGCGACAGAGAGCACCTAAAGGAACGCTTGGCCAACGAGCTGTC
  ACAGCTCCCCAAGGTGCGCCTGATCCGTGCTAGCAGGAGAGAGGGCCTAGTGCGAGCCCGGCTACTGGGA
  GCCTCTGCGGCCAGGGGCGAAGTGCTGACCTTCCTAGACTGTCACTGTGAATGTCATGAGGGGTGGCTGG
  AGCCTCTGCTGCAGAGGATCCACGAGAAGGAGTCCGCAGTCGTGTGCCCTGTTATCGATGTGATTGACTG
  GAACACCTTTGAGTACCTGGGCAACTCGGGAGAGCCGCAGATTGGCGGCTTTGACTGGCGGCTGGTATTC
  ACGTGGCACGTGGTTCCCCAGCGGGAGCGGCAGTCGATGCGGTCGCCGATCGATGTTATCAGGTCTCCAA
  CTATGGCTGGGGGCCTGTTTGCTGTGAGTAAGAGATATTTTGATTATCTGGGGTCTTACGATACAGGAAT
  GGAAGTCTGGGGAGGAGAAAACCTTGAGTTCTCCTTTAGGATCTGGCAGTGTGGTGGCACTCTGGAAACG
  CACCCGTGCTCCCATGTGGGCCACGTCTTCCCTAAGCAAGCTCCCTATTCCCGTAGCAAGGCCCTGGCCA
  ACAGTGTCCGAGCTGCAGAAGTGTGGATGGATGAATTTAAAGAACTCTACTACCACCGCAATCCCCAGGC
  CCGCCTGGAACCCTTTGGGGACGTGACAGAGAGGAAGAAGCTTCGGGCTAAGCTCCAGTGTAAAGACTTC
  AAGTGGTTCTTAGATACAGTGTACCCAGAATTGCACGTGCCAGAGGACAGGCCTGGCTTCTTTGGGATGC
  TCCAGAACAGAGGCCTAAGAGGGTACTGCCTTGACTACAATCCTCCCAATGAAAACCATGTTGAAGGCCA
  CCAGGTCCTTCTGTACCTCTGCCATGGGATGGGTCAGAACCAGTTTTTCGAGTATACGACCCGGAAAGAA
  ATACGCTATAACACCCGCCAGCCCGAAGCCTGCATCACCGTGGAGGACGGGAAGGATACCCTTGTCATGG
  ATCTCTGCCGAGAGACCGTCCCAGAGAACCAGGAGTTCATCCTACAGGAGGATGGCACTTTAGTTCACAA
  GCACAGCAGGAAATGTGTGGAGGCCACAGAGAAGGTGTTAGACAACGGCTTTGCACCATACTTACGGGAC
  TGTACCAACTCAGATAACCAGAGGTGGTTCTTCAAGGAGCGCATGTCGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172693
• Insert Size: 1731 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172693.3, NP_766281.1
• RefSeq Size: 2216 bp
• RefSeq ORF: 1731 bp
• Locus ID: 230145
• UniProt ID: Q8BGT9
• Cytogenetics: 4 B1
• Gene Summary: Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Has activity toward non-glycosylated peptides such as Muc5AC, Muc1a and EA2, and no detectable activity with Muc2 and Muc7. Displays enzymatic activity toward the Gal-NAc-Muc5AC glycopeptide, but no detectable activity to mono-GalNAc-glycosylated Muc1a, Muc2, Muc7 and EA2. May play an important role in the initial step of mucin-type oligosaccharide biosynthesis in digestive organs (By similarity).[UniProtKB/Swiss-Prot Function]