4930447C04Rik (NM_029444) Mouse Untagged Clone

CAT#: MC219203

4930447C04Rik (untagged) - Mouse RIKEN cDNA 4930447C04 gene (4930447C04Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: 4930447C04Rik
• Synonyms: 4921504I02Rik; A930035O15Rik; Six6as; Six6OS; Six6os1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219203 representing NM_029444
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATGATAATCTGTTTGTCAGTTTGGACAGGCTTTTGTTGGAATTTGTTTTCCAGTATGAGCAAGACA
  TAAGTATTAAAGAAGATACGATTCAAAGAATTAATAAATGCCTTGAAAGTATTAAAGAAAATAAAGCAAA
  TGTTTCTAAGCTACGTGAAGCTATAAACAAAGTAGATGAGGACATTGCTTTTCACTATAAACATAGTAAA
  GAAATCAAAGACAGCTGTAGTAACTGGAAGCCAACATGTGATGTTTTTCATAAACATGAAGATTATATAA
  AAGACCAGCTTACTGCTTACCAAGAAACCAATGAGAAAGACAAAAAAATGTATCATGATTATATATGTCA
  GTATGAAGATGTTTTGAAACAATACCAACTAAAATACTCAGAAACACGTTTTTCATGTAAATATTATGAG
  AAGAAAAAAGAACATGAAGAAATTAAAAATAGAGTGTTGGCATGTACTGAACAACTACAATTGAATGAAA
  CTATTCTTATGAAATTTCTAGTGCCTGCTCCTTTCCCATCTCTGACTAAATGGACATTATACGTAGTTAA
  TTTGAGATACAGAACACAAGATATTCTTAAACGTGCCAACAATTTTACCAAAAGATCATTTGAATTGGAG
  AAAGAAGCAGATGATATGGAAATAGAAATTAATTCTTTAAATAAGATGGCAAGACTTTTTGAAAGTAAAA
  CATTTTCAGAAGCTCTAGACGAAAAGAACAAAAATACAGAAAAGAGAAAAGAATTTGAAGAGAGAATTTT
  TGAAAAAGATGAGCAAGTATCAAACAGAAGCTCTCAAAATAGCCAATTGTTACTGCCATGTGAGTCTCAG
  AAATTTGTAAGAAATATGAATTCTTCAGAAGCAAGAGTTACAGATAAAAAAGAAGAAAGTTCAGCCAATC
  AGTCAAAGTTTGTTAGAAGTGATGTGAGACAAAAAGAAAATAATCCACAGATATTTAATGACTCTGGTAT
  GGATAGTAATTCAAAATCTTCACATATTCCAGCTGTTAAAAGTTCACAAGGTTTTATGCAATTCAGATTG
  AATCAACCAAATTACAATCAACGGATTGAAAAAGAACATATAGATGCTGAATGTGGAGATAAAGAGACAG
  TAAGACAAGTAAGAGAATCAAAATGTAGTACACAGGCCCTATACATTGAACACTTTGGAAAGTCCATAGA
  AAATAATAGTGTTGAAGAGGAAAGAGATGAGAATTTTCCTCAAACTCCTGAAACTCCTTCATTTCTAAGA
  ACTCCTGAAGCGCTGAAGACACCTGAATCTATGGAGAAAATGCAGTTCCCTAAAAGCCCCTTCTTTGAAA
  TAACTAAAAATGCAACATCTGAAGGACATAAACAAAAGGATTCTCCTGGATTTTCATTTCTTATGAGTTA
  CACCTCTAGATCACCTGGACTAAATTTATTTGATTCTTCTGTATCTGATTCAGAAATCTCATCGGATCAG
  TTTAATGAACATTACTCTGCGGTAAATCTAAATCCTTCTTCATCACAACAAGGAATTGGAAACTTATTTG
  GAAAATCAGAAGGAGAAGATGCCTTCACATTTTCTTTTTCATCAGACTCTTCTCACACATTTGGAGCTGG
  AAAAGATGACTTTAGTTTTCCATTTTCTTTTGAACAGGATCCAAGCACAATGACTTCTTCTTCCTCAAAA
  GATTTTTCATCCTCACAAAATAAAACACAATTTATGTTTTTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029444
• Insert Size: 1725 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029444.2, NP_083720.1
• RefSeq Size: 1910 bp
• RefSeq ORF: 1725 bp
• Locus ID: 75801
• UniProt ID: Q9CTN5
• Cytogenetics: 12 C3
• Gene Summary: Meiotic protein that localizes to the central element of the synaptonemal complex and is required for chromosome synapsis during meiotic recombination (PubMed:27796301). Required for the appropriate processing of intermediate recombination nodules before crossover formation (PubMed:27796301).[UniProtKB/Swiss-Prot Function]