Crmp1 (NM_007765) Mouse Untagged Clone

CAT#: MC219157

Crmp1 (untagged) - Mouse collapsin response mediator protein 1 (Crmp1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Crmp1
• Synonyms: CRMP-1; Dpysl1; DRP; DRP-1; Ul; ULIP-3; Ulip3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219157 representing NM_007765
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCATCAGGGGAAGAAGAGCATCCCGCACATCACCAGTGACCGGCTCCTCATCAGAGGTGGACGCA
  TCATCAATGATGACCAGTCCTTCTACGCCGATGTCTACCTAGAAGATGGACTCATAAAACAAATAGGAGA
  GAACCTGATTGTTCCTGGTGGAGTGAAGACCATCGAGGCGAATGGCCGAATGGTCATTCCCGGTGGCATT
  GATGTCAACACTTACCTGCAGAAGCCCTCCCAGGGCATGACCTCGGCTGATGACTTCTTCCAGGGCACTA
  AAGCAGCGCTGGCAGGTGGAACCACGATGATCATTGACCACGTTGTTCCTGAACCTGGGTCCAGCTTGTT
  GACTTCCTTTGAGAAATGGCACGAAGCAGCAGACACCAAATCCTGCTGTGACTATTCCCTCCACGTGGAC
  ATCACAAGCTGGTATGATGGTGTTCGGGAAGAGCTGGAGGTGCTGGTGCAGGACAAAGGTGTCAACTCCT
  TCCAAGTCTACATGGCGTATAAGGACCTGTACCAGATGTCTGACAGCCAGCTGTATGAAGCCTTCACCTT
  CCTTAAGGGTTTGGGAGCTGTGATCTTAGTCCATGCAGAAAATGGAGATTTGATAGCTCAGGAACAAAAA
  CGGATCCTGGAGATGGGCATCACGGGTCCCGAGGGTCATGCTCTGAGCAGACCCGAGGAGCTGGAGGCCG
  AGGCTGTGTTCCGGGCTATTGCCATTGCAGGCCGGATCAATTGCCCTGTGTACATCACCAAGGTCATGAG
  CAAGAGTGCAGCGGACATCATCGCACTGGCCAGGAAGAAAGGCCCTCTTGTCTTCGGTGAGCCCATAGCC
  GCCAGCCTGGGAACCGATGGCACCCACTACTGGAGCAAGAACTGGGCCAAGGCAGCTGCATTTGTGACTT
  CCCCTCCCCTGAGCCCAGACCCCACCACTCCTGACTACTTGACCTCCTTGCTGGCCTGTGGAGACTTGCA
  GGTCACAGGTAGTGGCCACTGTCCCTACAGTACTGCTCAGAAGGCTGTGGGCAAGGACAACTTCACTCTG
  ATCCCTGAGGGTGTCAATGGTATAGAAGAGCGGATGACCGTTGTCTGGGACAAGGCAGTGGCTACTGGCA
  AGATGGATGAGAACCAGTTTGTAGCCGTCACCAGCACCAACGCAGCCAAGATCTTCAACCTGTACCCGAG
  GAAAGGTCGGATCGCTGTGGGCTCCGATGCTGACGTAGTCATCTGGGACCCAGATAAGATGAAGACCATA
  ACAGCCAAAAGCCATAAATCAACTGTGGAGTACAACATCTTTGAGGGCATGGAGTGCCACGGCTCCCCCC
  TGGTGGTCATCAGTCAGGGCAAGATTGTCTTTGAGGATGGAAACATCAGTGTCAGCAAGGGCATGGGCCG
  CTTCATCCCTCGGAAGCCATTCCCAGAGCATCTCTACCAGCGTGTCAGGATCAGAAGCAAGGTTTTCGGG
  TTGCATAGTGTTTCCAGGGGCATGTACGATGGGCCTGTGTACGAGGTGCCAGCTACACCCAAACATGCTG
  CTCCTGCTCCTTCTGCCAAATCCTCGCCTTCTAAACACCAACCCCCACCCATCCGGAACCTCCACCAGTC
  CAACTTCAGCTTATCAGGTGCCCAGATAGATGACAACAATCCAAGGCGTACAGGCCACCGCATTGTGGCG
  CCCCCTGGTGGCCGCTCCAACATCACCAGCCTCGGTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_007765
• Insert Size: 1719 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007765.4, NP_031791.3
• RefSeq Size: 2968 bp
• RefSeq ORF: 1719 bp
• Locus ID: 12933
• UniProt ID: P97427
• Cytogenetics: 5 19.96 cM
• Gene Summary: This gene encodes a protein that is part of the collapsin response mediator protein family. The family is comprised of five, homologous cytosolic phosphoproteins that are expressed in developing and adult nervous tissue and mediate signaling to transduce responses to extracellular cues. This protein is a Semaphorin 3A signaling molecule that regulates collapse of the growth cone. The growth cone mediates axonal pathfinding in neurons. This protein is reported to represent a new class of microtubule-associated proteins. In humans this protein is reported to inhibit cancer cell invasion. In mouse deficiency of this gene may be associated with impaired spatial memory performance. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]
  Transcript Variant: This variant (2) contains an alternate 5'-most exon and initiates translation at an alternate start codon compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1.