Siglecf (NM_145581) Mouse Untagged Clone

CAT#: MC219125

Siglec5 (untagged) - Mouse sialic acid binding Ig-like lectin 5 (Siglec5), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Siglecf
• Synonyms: mSiglec-F; Siglec5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_145581, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCGGTGGGCATGGCTGCTGCCCCTGCTGTGGGCAGGGTGCCTGGCTACGGACGGTTATTCGCTCTCAG
  TGACTGGTTCAGTGACAGTGCAGGAGGGCCTGTGCGTCTTTGTAGCCTGCCAAGTCCAGTACCCCAATTC
  CAAAGGTCCTGTCTTTGGCTACTGGTTCCGGGAGGGGGCCAATATATTCAGCGGCTCTCCAGTGGCTACA
  AATGACCCACAGCGATCAGTACTGAAGGAAGCTCAAGGTCGATTCTATCTCATGGGGAAGGAAAATAGTC
  ATAATTGCTCCCTGGACATCAGAGATGCACAGAAAATCGACACAGGGACGTACTTCTTCAGATTGGATGG
  TTCTGTGAAATACAGTTTTCAGAAGTCAATGCTCTCTGTGCTTGTGATAGCTCTGACTGAGGTCCCTAAC
  ATCCAAGTCACATCTACCCTGGTGTCTGGCAATTCTACCAAACTGCTCTGTTCTGTG:CCCTGGGCCTGT
  GAGCAGGGGACACCCCCCATCTTTTCCTGGATGTCATCTGCCCTCACTTCCCTGGGCCACAGGACCACCC
  TCTCCTCAGAGCTGAACCTCACACCCAGGCCTCAGGACAATGGCACCAACCTCACCTGCCAGGTGAACTT
  ACCTGGCGCTGGTGTTACTGTGGAGAGGACTCAACAGCTCAGTGTCATCTATGCTCCACAGAAGATGACC
  ATCAGGGTGTCCTGGGGAGATGACACAGGAACTAAAGTCCTGCAGAGTGGAGCATCTCTGCAGATCCAAG
  AGGGTGAGTCCCTGAGCCTGGTCTGTATGGCTGACAGCAATCCCCCTGCCGTGCTGAGTTGGGAGCGCCC
  GACCCAAAAGCCCTTCCAGCTCTCTACCCCCGCGGAACTGCAACTGCCACGGGCGGAGTTGGAGGACCAG
  GGAAAATACATCTGCCAAGCTCAGAACAGTCAGGGTGCTCAGACAGCCTCTGTGAGCCTCAGCATAAGGA
  GCCTTCTACAGCTGCTGGGACCCTCCTGCTCCTTCGAGGGTCAAGGTCTGCACTGCAGCTGCTCCTCCAG
  AGCCTGGCCTGCCCCCTCCCTGCGCTGGCGGCTGGGGGAGGGGGTGCTGGAGGGGAACAGCAGCAATGGC
  TCCTTCACGGTTAAGTCCAGCTCCGCAGGACAGTGGGCCAACAGCTCCCTGATCCTCAGCATGGAATTCA
  GCTCTAACCACAGACTCAGCTGTGAAGCTTGGAGTGACAATAGAGTTCAGAGAGCCACTATCTTGCTGGT
  GTCAGGCCCAAAGGTCTCACAGGCAGGCAAATCAGAAACCAGTAGAGGAACGGTCCTGGGGGCCATCTGG
  GGAGCTGGTCTTATGGCCTTGCTTGCTGTCTGCCTCTGCCTCATCTTCTTCACAGTGAAGGTCCTCAGGA
  AGAAATCAGCCCTGAAAGTAGCAGCTACGAAAGGCAACCATCTTGCCAAGAACCCTGCCTCCACCATAAA
  TAGTGCAAGCATAACTTCATCCAACATTGCGTTGGGATACCCAATCCAGGGTCATCTGAATGAACCAGGG
  TCACAGACCCAGAAAGAACAGCCACCCCTTGCCACAGTCCCAGACACCCAAAAGGATGAGCCTGAACTCC
  ACTATGCCTCCCTCTCCTTCCAAGGCCCGATGCCCCCGAAGCCTCAGAACACGGAGGCTATGAAATCTGT
  ATACACAGAGATCAAGATCCACAAGTGCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_145581
• Insert Size: 1710 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC145987, AAI45988
• RefSeq Size: 2253 bp
• RefSeq ORF: 1710 bp
• Locus ID: 233186
• UniProt ID: Q920G3
• Cytogenetics: 7 B3
• Gene Summary: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) is the longer transcript and encodes a longer isoform (1).