Nepro (NM_145972) Mouse Untagged Clone

CAT#: MC219112

BC027231 (untagged) - Mouse cDNA sequence BC027231 (BC027231), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nepro
• Synonyms: Nepro
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219112 representing NM_145972
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCTGCGGTGCGGCCGGGTGCGGAGCCGTGGAACCGCGTGAGGATCCCCCAGGCGGGGAACTGCA
  GCACGCTGACCGTGCGGGACCCCAGCGCCACCCTGGATATCTGCACTGCAGCTGTCACTAAAGGGTGCCA
  CCTTGTCACACAGTCACTCAAGAGCCAGACCTTGGATGCGGAAGTAGATGTGTTATGCTCAGTTCTCTAC
  AGCAATCACAACAGGTTGGGCCACCACAAGCCCCATTTGGCCCTCAGACAGGTAGAACAGTGCTTAAAGC
  GTTTGAAACATATGAATTTGGAGGGCTCAATTGAAGACCTGTCACAGTTGTTGTCTGCCAATGCAACTCA
  GCCTGGAGCTACAGAAAACCGCGTCGTCCCCAGCCAGCCGGTGGTGGAGGTGGTGCTGATGAAGGTTTTG
  GGAGGCTGCAAGTTGTTACTGCGCTTGCTGGACTGCTGCTGCAAGGCATTTCTGTTGACTGTGAAGCACT
  TAGGGCTAAAGGAATTCATTATTTTGAACCTTGTGATGGTTGGACTGGTGAGCAGGCTATGGGTTCTCCA
  TAAAGGACTTTTGAGAAGGCTCATTTCCTTATATGAGCCATTACTCAGCTTGCGTCAGGAGATCTCCAGC
  ATTCATCCCATGCCTTACTTCAAAGATTTTGCCTTTCCCTCTGACATCACTGACTTTCTAGGCCCCTCTT
  ACCTTGAAGTGTTCAAGGTAAAAACACCTGCGGCTTCTGCTACTAAAGGAGTAACAAAGTTGCTAAATAA
  ACTGTTTTTAATGAGAGAGCAGCTACCAAAGATGAATGAAGACACTTTAGATAGACTTTCCAAACCATCT
  GAACAAATGACAAGCAATCCACAGAGCACTGTGGACCTGGGCCAGCCAGTGAAAGCCTGTAAGAGGACCA
  GAAAAGAAAAGCCATTGGGATTTGACCTTCGGGCTTTTTGCACAAGACTTGGAAACAAAGCGACTCAGGA
  GACCAACCGTGACTTTAAATATTCTCAGTCCAAACTGAAGACAACCAAACTTCCCTCTCAGCAACTGAGA
  ACTCACTGGGCTAACGATACTGTGCAAAGAATCCGAAAGACCAAGACGTTCGCCCAACTTTCTGAAGAAA
  TTGAAATGGCAATTGTGTGGTCCCGGAGCAAAAAGCTCAAGACTCAGGCTACCTTTCTGGGCAACAAACT
  TCTTAAGAGTAATAGGTTTAGACATGTGGAATCTCAAGGCTATAGTTTGACAAAGAAGCTCCAGTGCATG
  AAAACATCTCTCTGCAACTGCCTTCTCCGAGGCTCTCGTACCAGCACTTCAGAGCATCCTCCCAGACAAA
  GAAGATCAAAGTATAAAGTTTTATCAAGACAAAGGAAACCGCAGAGAAAGTTACAGTCAACTCTTTTAAA
  GGAAACCCAGCAGGTCCCTGAAGGGACTCTGAAGAACACTAGGGACAGCAGTGCTAAGAGGAGATGCTCT
  GGGACAGTTCAGAGGTCAGATGTCTGTCCTAATGGAAAGCAGGTCTTGAGAAAACTTGCAAAGCCTGACC
  TAAAAACAAAGGTGGTGGTCCACGGGAATCTTACAGGGGGCAGTAGAAATGAATCTGGCTTTCAGGCGAA
  GACACAGATGCACACACACAATGCGCCAGACACTGCTAAAGAGGCAGATGACATTGATGATATTTTTGCT
  TTGATGGGAGTTTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145972
• Insert Size: 1695 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145972.4, NP_666084.1
• RefSeq Size: 2866 bp
• RefSeq ORF: 1695 bp
• Locus ID: 212547
• UniProt ID: Q8R2U2
• Cytogenetics: 16 B4
• Gene Summary: May play a role in cortex development as part of the Notch signaling pathway. Downstream of Notch may repress the expression of proneural genes and inhibit neuronal differentiation thereby maintaining neural progenitors (PubMed:19906856). May also play a role in preimplentation embryo development (PubMed:26178919).[UniProtKB/Swiss-Prot Function]