Asb14 (NM_080856) Mouse Untagged Clone

CAT#: MC219109

Asb14 (untagged) - Mouse ankyrin repeat and SOCS box-containing 14 (Asb14), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Asb14
• Synonyms: Asb-14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219109 representing NM_080856
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAAAGTTGAAAAAGCCTTTTCACAACTGGAAAAATATTTTAATTCCTTCTGACTTGGAAATCAACC
  TATCCAAGCTTCGTATGGATCCCTCCGGCAGTGACGAGGATGCAGATGCCGACTTTGACACCCAGGTCAT
  CATCCAACAGAGTCTGCTGGATGTTTACAAGCCAGGGATAGCTCAGCACACGCCAGAAGCGGCGAGACTC
  CATTCCTTCCCAAGTGATGATTATAAGAAGATAGCTGAAGCCATCGAGACAGGTAAGGAAGATGCATTGG
  CAGGCTTTGCCAAGTACCATCCAGCCTTTGATGAAGCCAATGGGAATGGCTGGCTTCCTCTGCATAAGGC
  AGCAGTGCAGTTAAACAAGAACATCTTAGAAATAACCATGAACGCTTCCGAACCCAGTACGTGGGAACGA
  ACCACTCACAACGGTGAAACAGCACTTTTTTTGGCTGTCAGCAGTAGCCTCCTGGAAAACGCCCACTTTC
  TCCTTCTCAAAGGCTGTAATCCAAACGCTAAAACTTCTGAAGGCAATTCTCCTCTTCTTACAGCTGTTCT
  GAAAGACGCCTACGACATGGCCACCTTGCTGATCAGCCATGGAGCAGATGTCAATCTCCGATGTGCCAAT
  GAGAGGACAGCTCTCCATGAAGCTGCCAAGCTGGGCAGACTGGACATGGTAAAACTGATGCTGGCTTCTG
  GAGCATACCCAGATGCACGGAGCTCCTACGGATTCACGCCCCTTGCCCTCGCTGCTCAAGGTGGACACAC
  TGGGATCATGCAACTACTACTGCAGAAAGGAGCTGACGTTCACAGTCAGGCCTCTGACTCGTCCTCTGTG
  TTACTTGAAGCTGTTAGAGGAGGGAATCCGGAAGCTGTGAGTCTGTTGTTAGAGTATGGAGCTGATGCCA
  ACATTCCTAAGAGTTCAGGCCACCTGCCCATCCACGTGGCAGCTGACAAAGGCCATTTCCTAGCTCTAAA
  GGTATTGGTCCCAGTCACAGACATCGCAGCCATCAAGAAGAGCGGGATCAGTCCGGTTCACTGCGCAGCA
  GCTGGGGCGCACCCCCACTGCCTGGAACTTCTCATTCAGGCTGGATTTGATGTGAATTTCATGCTGGATC
  AGAGAATCCGTAAACATTATGATGATCAACGGAAGTCAGCCTTGTATTTTGCTGTTTCAAACGGTGACCT
  CCCTTCAGTTAAGCTGCTTCTGAGTGCTGGAGCTCTACCCAATCAAGACCCAGTCAACTGCCTACAGATA
  GCTCTTAGGATGGGCAACTATGAGCTCATAAGTCTATTACTGAGGCACGGGGCCAACGTCAACTACTTCT
  GCCGCGTTAACCCTTTGCATTTCCCATCAGCGCTACAATACACATTGAAAGATGAAGTCATGCTCAGGAT
  GCTGTTGAATTATGGCTATGACACTGAGCGATGTTTTGATTGTCCTCATGGAGAAAGGGTTCATCGGTTT
  TGTACTTTTGAAGGCTGGACATCTACAGTTATTAAAGATACTATGTTTTGTGAAGTCATAACTTTGTCAT
  GGCTACAGCATCTCTCTGGGAAAGTTGTTCGTGTGATGCTTGATTATGTTGATCAAGTTCAAATCTGTTC
  AAAATTGAAAGCTGTGCTGGAAAAACAAAGGCTCTGGCCAGAAATACATTTTATCTTGAGTGAGTATCGT
  TCTCATAGAAAATTCCTACCAAAACACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_080856
• Insert Size: 1710 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080856.4, NP_543132.3
• RefSeq Size: 1815 bp
• RefSeq ORF: 1710 bp
• Locus ID: 142687
• UniProt ID: Q8C6Y6
• Cytogenetics: 14 A3
• Gene Summary: May be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate 3' splice pattern, compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. CCDS Note: The coding region has been updated to represent an alternate splice site between exons 3 and 4 which includes an intron with TT^AG splice junctions that is conserved in other species. The update also extends the N-terminus by 129-aa, and changes the C-terminus.