Mccc2 (NM_030026) Mouse Untagged Clone

CAT#: MC219103

Mccc2 (untagged) - Mouse methylcrotonoyl-Coenzyme A carboxylase 2 (beta) (Mccc2), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mccc2
• Synonyms: 4930552N12Rik; MCCB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219103 representing NM_030026
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGGCGCGCTGAGGTCAGCTCTGCGACCGTGCTGTCGGGCTGCTGTACCCCCGCAGCGTGCCTATC
  ACGGGGACTCAGTGGCCCGACTGGGCACCCAGCCTGACTCGGCCTCCTCCACCTACCAGGAGAACTATGA
  GCAGATGAAAGCTTTAGTGAGTCAACTTCATGAGCGAGCCCAGTATGTGAGACTAGGAGGGAGTGAGAAG
  GCCCGGGCGCGGCACACATCCAGAGGAAAACTGCTCCCCAGAGACAGAATCGACAATCTCATAGACCCAG
  GGTCTCCCTTTCTGGAATTCTCCCAGTTTGCAGGTTACCAGTTATATGGCGACGAGGAAGTCCCCGCCGG
  TGGCATCATCACGGGCATTGGGCGAGTGTCGGGGGTAGAATGCATGATTGTGGCTAACGATGCCACCGTC
  AAAGGAGGCACCTACTACCCAGTGACTGTGAAAAAGCACGTGCGGGCACAAGAGATTGCGCTGCAGAACC
  GACTCCCATGCATCTATCTGGTTGACTCAGGAGGGGCCAACTTACCTCGGCAAGCAGACACCTTTCCAGA
  CCGGGACCACTTTGGCCGCATATTCTATAATCAGGCAATTATGTCTTCTAAAAACATCACACAGATAGCT
  GTGGTCATGGGCTCCTGTACGGCAGGAGGTGCGTATGTGCCTGCAATGGCTGATGAAAACATCATTGTAC
  AGAAGCAGGGTACCATCTTCTTGGCTGGACCACCCTTGGTGAAGGCAGCTACAGGTGAAGAGGTGTCCGC
  TGAGGATCTCGGAGGTGCCGACCTGCATTGCAGAAAGTCTGGGGTCACTGACCACTATGCTTTGGACGAC
  CACCACGCCCTCCACTTAACAAGGAAGGTTGTGAGGAGTCTCAATTATCAGAAGAAAATGGATGTTACCA
  TTGAGCCTTCTGAGGAGCCCTTGTTCCCTGCAGATGAGCTCTATGGAATAGTTGGTGCAAACCTGAAGAG
  GAGCTTTGATGTCCGAGAGGTCATTGCTAGAATCGTGGATGGAAGCAGATTCAATGAGTTCAAGGCCTTA
  TATGGAGACACCCTAGTTACAGGATTTGCTCGAATATTTGGGTACCCCGTTGGCATCATTGGAAACAATG
  GAGTTTTGTTTTCTGAATCTGCTAAGAAGGGTGCTCACTTCGTCCAGCTGTGCTGCCAGAGAAATATCCC
  TCTGCTGTTTCTTCAGAATATCACCGGATTCATGGTTGGCAGAGACTATGAAGCTGAAGGGATTGCCAAG
  GATGGTGCCAAAATGGTAGCTGCAGTAGCCTGTGCCAAAGTGCCTAAGATAACTGTCATAATTGGGGGAT
  CGTACGGGGCTGGAAACTACGGAATGTGTGGCAGAGCATACAGTCCAAGGTTTCTCTACATGTGGCCAAA
  TGCCCGCATCTCTGTGATGGGAGGAGAGCAGGCAGCCACGGTGTTAGCCACAGTGGCTAGAGACCAGAAA
  GCACGCGAGGGGAAGCAGTTCTCCAGTGCAGAGGAGGCAGCTTTGAAAGAACCCATCATTAAGAGGTTCG
  AAGAGGAAGGGAACCCTTACTACTCTAGTGCAAGGCTGTGGGACGATGGGATTATTGACCCGGTGGACAC
  CAGGCTGGTCCTGGGGCTCAGCCTCAGTGCAGCCCTCAATGCGCCCATCCAGAGGACTGACTTTGGCATC
  TTCAGGATGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_030026
• Insert Size: 1692 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030026.2, NP_084302.1
• RefSeq Size: 2146 bp
• RefSeq ORF: 1692 bp
• Locus ID: 78038
• UniProt ID: Q3ULD5
• Cytogenetics: 13 D1
• Gene Summary: Carboxyltransferase subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3-methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism.[UniProtKB/Swiss-Prot Function]