Cspg5 (NM_001166273) Mouse Untagged Clone

CAT#: MC219066

Cspg5 (untagged) - Mouse chondroitin sulfate proteoglycan 5 (Cspg5), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cspg5
• Synonyms: CAL; Caleb; NG; Ngc
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219066 representing NM_001166273
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCGAGCTGGAGGCGGGGGCCCGGACTGGGGGCCGCCGCCAGTGCTGCTGCTTCTGGGGGTCACGC
  TGGTGCTCACCGCTGGGGCCGTACCGGCACGGGAAACAGGCAGTGCGATCGAGGCTGAAGAGCTGGTGAG
  GAGCAGCCTGGCATGGGAGTCGCGTGCCAATGACACGCGGGAGGAAGCCGGCCTGCCAGCAGCTGGGGAA
  GATGAGACCTCGTGGACAGAGCGGGGCAGTGAGATGGCTGCGGTGGGCCCTGGGGTCGGGCCAGAGGAGG
  CACTAGAGGCATCGGCTGCAGTGACTGGCACTGCCTGGCTAGAGGCAGATGGCCCAGGCCTGGGTGGAGT
  GACTGCAGAGGCTGGCAGTGGCGACGCCCAGACCCTTCCAGCTACGCTCCAGGCTCCTGATGAGGCCCTT
  GGGTCATCTACAATGCCCCCTGCCATCCCTGAGGCTACTGAAACCAGTGGACCTCCCTCCCCTGCTGTCC
  ATGATAAGCCTAGTGTAGGCCCTGAACTCCCTAAAGAGATCCCCTTGGAGGTTCGGCTGAACCTGGGAGG
  CAGCACACCAGAGCCCACTTTTCCCCTTCAGGGCACTCTCGAGACCCAACCAGCCTCAGATATAATTGAC
  ATTGATTACTTTGAAGGATTGGATAGTGAGGGTCGTGGTGCAGACATGGGCAGCTTCCCGGGGTCACCAG
  GAACCTCAGAAAATCACCCTGATACCGAAGGAGAGACCCCTTCCTGGAGCCTGCTTGATTTGTATGATGA
  CTTCACCCCTTTTGATGAGTCTGATTTCTACCCCACCACATCCTTCTATGATGATTTGGAAGAGGAGGAA
  GAAGAGGAGGAGGATAAGGATACAGTAGGAGGTGGAGACCTGGAAGATGAAAACGACCTTCTCCTGCCCT
  CTCAAAAGCCTGGTGTGGGGCCTGGGACAGGACAGCCCACCAACCGGTGGCATGCTGTTCCCCCACAGCA
  TACTCTGGGGATGGTACCTGGCAGCAGCATCTCTCTTAGGCCCCGCCCCGGAGATCCAGGCAAGGACCTG
  GCCTCAGGAGAAAATGGCACAGAGTGCCGAGTTGGCTTCGTCAGGCACAATGGCTCCTGCCGGTCAGTCT
  GTGACCTCTTTCCGAGTTACTGTCACAACGGCGGCCAGTGCTACCTGGTGGAGAACATAGGGGCTTTCTG
  CAGGTGTAACACCCAGGACTACATCTGGCACAAGGGGATGCGCTGTGAGTCCATCATCACGGACTTCCAG
  GTGATGTGCGTGGCCGTTGGCTCGGCTGCTCTCGTGCTTCTCCTCCTGTTCATGATGACTGTGTTCTTTG
  CCAAGAAGCTCTATCTGCTCAAGACTGAGAATACCAAGCTGCGGAGGACCAATAAATTCCGGACCCCATC
  TGAGCTCCACAACGACAACTTCTCCCTCTCCACCATTGCCGAGGGCTCTCATCCAAATGTAAGGAAATTT
  TGCGACACTCCCCGTGTCTCCTCCCCCCATGCCCGTGCCTTGGCTCACTATGATAACATTGTCTGTCAGG
  ACGACCCCAGCGCTCCCCACAAAATCCAGGACCCTCTCAAGTCCCGCCTGAAGGAGGAAGAGTCCTTTAA
  CATCCAGAACTCCATGTCACCCAAACTTGAGGGTGGCAAAGGTGACCAGGATGACTTGGGGGTGAACTGT
  CTGCAGAATAACCTAACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001166273
• Insert Size: 1701 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001166273.1, NP_001159745.1
• RefSeq Size: 3881 bp
• RefSeq ORF: 1701 bp
• Locus ID: 29873
• UniProt ID: Q71M36
• Cytogenetics: 9 F2
• Gene Summary: This gene encodes a chondroitin sulfate proteoglycan. The encoded protein has been termed a 'part-time' proteoglycan, as chondroitin sulfate chains appear to be attached to the protein in the developing but not the adult cerebellum and retina. It is thought that this protein plays roles in dendrite branching and synapse formation. [provided by RefSeq, Oct 2009]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a longer protein (isoform b), compared to isoform a. Isoform b is also known as NGC-III and mCALEBb. Variant 2 contains an in-frame start site 223 codons upstream from the currently annotated site, but Kozak sequence and signal peptide considerations support use of the downstream AUG.