Pde4b (NM_001177980) Mouse Untagged Clone

CAT#: MC219056

Pde4b (untagged) - Mouse phosphodiesterase 4B, cAMP specific (Pde4b), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pde4b
• Synonyms: Dpde4; dunce; R74983
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219056 representing NM_001177980
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGAGCAAGGGGGCACCGTCAGTGGCGCCGCGAGCAGCCGAGGTGGAGGAGACTCGGCTATGGCCA
  GCCTGCAGCCACTGCAGCCTAACTACCTGTCTGTGTGTTTGTTTCCAGAAGAATCATATCAGAAACTAGC
  AATGGAGACGCTGGAGGAACTAGACTGGTGCCTAGACCAGCTAGAGACCATCCAGACCTACCGCTCTGTC
  AGCGAGATGGCTTCAAACAAGTTCAAAAGGATGCTGAACCGGGAGCTGACACACCTCTCAGAGATGAGCA
  GATCAGGGAACCAGGTGTCTGAGTACATTTCAAACACGTTCTTAGACAAGCAGAACGATGTGGAAATCCC
  ATCTCCCACGCAGAAGGACAGGGAGAAGAAGAAGAAGCAGCAGCTCATGACCCAGATAAGTGGAGTGAAG
  AAACTGATGCACAGCTCAAGCCTGAACAACACAAGCATCTCACGCTTCGGAGTCAACACGGAAAATGAGG
  ATCATCTAGCCAAGGAGCTGGAAGACCTGAACAAATGGGGCCTTAACATCTTCAATGTGGCTGGGTACTC
  ACATAATCGGCCCCTTACGTGCATCATGTATGCAATATTCCAGGAAAGAGACCTTCTGAAGACGTTTAAA
  ATCTCATCTGACACCTTTGTAACCTACATGATGACTTTAGAAGACCATTACCATTCTGATGTGGCATATC
  ACAACAGCCTGCATGCTGCTGACGTGGCCCAGTCAACTCACGTTCTCCTTTCTACGCCGGCACTGGATGC
  TGTCTTCACAGACCTGGAAATCCTGGCTGCCATTTTTGCAGCTGCCATCCATGATGTCGATCATCCTGGA
  GTCTCCAATCAGTTTCTCATCAATACAAATTCTGAACTTGCTTTGATGTATAATGATGAATCTGTTCTGG
  AAAACCATCACCTTGCTGTGGGATTCAAATTGCTACAAGAGGAACACTGCGACATCTTTCAGAATCTTAC
  CAAGAAGCAACGCCAGACACTCAGGAAAATGGTGATTGACATGGTGTTGGCAACTGATATGTCCAAACAC
  ATGAGCCTCCTGGCAGACCTTAAAACAATGGTAGAAACCAAGAAGGTGACAAGCTCCGGTGTTCTCCTCC
  TGGACAACTATACTGACCGGATACAGGTTCTTCGCAACATGGTACACTGTGCAGACCTGAGCAACCCCAC
  CAAGTCCTTGGAATTGTATCGGCAATGGACCGATCGTATCATGGAGGAGTTTTTCCAGCAGGGAGACAAA
  GAACGGGAGAGGGGAATGGAGATTAGCCCAATGTGTGATAAGCACACAGCTTCTGTGGAAAAATCCCAGG
  TTGGTTTCATTGACTACATTGTCCATCCACTGTGGGAGACCTGGGCAGACCTGGTTCAACCGGATGCTCA
  AGATATTCTGGATACACTAGAAGATAACAGGAACTGGTACCAGAGTATGATACCCCAGAGCCCTTCCCCG
  CCACTGGATGAGAGGAGCAGGGACTGCCAAGGCCTGATGGAGAAGTTTCAGTTTGAACTGACCCTTGAGG
  AAGAGGATTCTGAGGGACCGGAAAAGGAGGGAGAAGGCCACAGCTATTTCAGCAGCACAAAGACGCTTTG
  TGTGATTGATCCAGAGAACAGGGATTCTCTGGAAGAGACTGACATAGACATTGCAACAGAAGACAAGTCT
  CCGATCGACACATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001177980
• Insert Size: 1695 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177980.1, NP_001171451.1
• RefSeq Size: 4206 bp
• RefSeq ORF: 1695 bp
• Locus ID: 18578
• Cytogenetics: 4 46.99 cM