Sptlc3 (NM_175467) Mouse Untagged Clone

CAT#: MC219046

Sptlc3 (untagged) - Mouse serine palmitoyltransferase, long chain base subunit 3 (Sptlc3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sptlc3
• Synonyms: C130053K05Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219046 representing NM_175467
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAATCTGAACGACAGTGCTGTTACAAATGGGACTCTACACAATCCTAAGACCCAGCAAGGAAAAA
  GGCAAAGCACTGGTTGTGTGAAAAATGGAATCTCAAAGGAAGCCCAGCAAAATAGGAAGGCATATGCTGA
  GGATAAGCCAGTCTTTGAGCCCTACCAGGAAGCACCACTTTATGTCTACGTTTTAACTTACATGGGATAT
  GGAATTGGAATCCTATTCGGCTATCTCAGAGACTTTATGAGAAACTGGGGAATTGAAAAATGCAATGCAG
  CTGTAGAGCGAGAAGAACAAAAAGACTTTGTGCCGCTGTATCAGGACTTTGAGAACTTTTATAAGAGGAA
  CCTTTACATGAGAATCAGAGACAGCTGGAGTCACACGGTCTGCAGTGCCCCAGAGCCTTACATGAATGTC
  ATGGAGAAAGTGACTGATGACTATAACTGGACATTCAGGCATACTGGAAAAGTTATAGAAAATATCATCA
  ACATGGCCTCCTACAACTACCTTGGGCTTGCAGGGAAATATGATGATTCGATGGTGAGAGTTAAGGATAC
  TTTAGAAAAGTATGGTGTAGGTGTGGCCAGCACCAGAAATGAAATGGGTACCTTGGATATACACAAGGAG
  TTGGAGGATCTCATGGCTGAGTTCTTGAATGTAGAAGCAGTGATGTCTTTTGGAATGGGATTCGCAACCA
  ACGCAATGAATATCCCTGTATTTGTTGGAAAGGGATGCCTCATTTTAAGTGATGAGTTTAACCACACTTC
  AGTCATCCTAGGATCTCGACTCTCAGGTGCAGTCATAAGACCCTTCAAACACAATAATGCAGAAAACCTA
  GAGAAGCTCCTGAGAGAAGCTATCATCCGTGGCCAGCCTGGCACAGGGAGAGCTTGGAAAAAGATTCTCA
  TCGTGGTAGAGGGCGTCTACAGCATGGAAGGTTCCATTGTGAACCTTGCCCAGATAGTGGCTCTAAAGAA
  GAAATACAAGGCTTACCTGTACATTGATGAAGCTCACAGTATTGGCTGTACAGGCCCCACGGGACGAGGC
  GTCAGAGAGTTATTTGGACTGGACCCTGAAGATATTGACGTATACATGGGTACATTCACCAAGAGCTTCT
  CGGGCTCAGGAGGATATATAGGCGGGAAAAAGGAAATTGTGGATTATTTACGGATGCAGTCACATAGCAC
  TACTTATGCTACATCCATGAGTCCCGTAGTAGCAGCACAACTCATCAGATCATTGAAGATAACTATGGGA
  TATGAGGGGAACATTGGAGGTATGGAGAGAATACAGCAACTTAAGGAAAACATAAAATACTTCCGACGGC
  GACTGAAAGAGATGGGATTCATCATCTATGGCAATGATTTCTCTCCGGTGATCCCTGTGCTCCTCTATAT
  GCCCGCTAAAGTGTCTGCTTTCAGCAGATTTTTGCTTAAGAAAAAGATCTCAGTGGTGGTTGTTGGATTT
  CCAGCTACTTCCCTGCCAGAAGGTCGGGCCAGGTTCAGCATGTCCTCTGCACATACTCGGGAGATGTTAG
  ACACAGTTTTGGAAGTCGTTGATGAGCTCGGTGATCTCCTGAACGTGAAATATTTCCCACTCAAGAAATC
  AGGGCGTGCTATACTCTACAATAAGGAGGGTTTTGACAATGAAGCAAGCTTTGAAGAGATGCACTCTGAA
  CCGGAAGCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175467
• Insert Size: 1692 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175467.3, NP_780676.1
• RefSeq Size: 2759 bp
• RefSeq ORF: 1692 bp
• Locus ID: 228677
• UniProt ID: Q8BG54
• Cytogenetics: 2 F3
• Gene Summary: Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. SPT complexes containing SPTLC3 generate shorter chain sphingoid bases compared to complexes containing SPTLC2. The SPTLC1-SPTLC3-SPTSSA isozyme uses C12-CoA, C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. On the other hand, the SPTLC1-SPTLC3-SPTSSB has the ability to use a broader range of acyl-CoAs without apparent preference.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein.