Mks1 (NM_001039684) Mouse Untagged Clone

CAT#: MC219030

Mks1 (untagged) - Mouse Meckel syndrome, type 1 (Mks1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mks1
• Synonyms: AK190930; avc6; B8d3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219030 representing NM_001039684
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAAGCGGTCTGGAGCACTGACACCGGCGAGGCCGTGTATCGCTCCCGGGACCCCGTGCGCAACC
  TGCGCCTCCGAGTTCATCTGCAAAGAATCACATCAAGCAACTTCCTTCATTACCAGCCTGCTGCCCAGAT
  GGGGAAGGACCTCATAGACTTGGCCACTTTTAGGCCTCCGCAAGCTGCTAGTGGACACCGCCCTGACGAA
  GAAGAGGAGGAAGAGGTTGTAATTGGATGGCAGGAAAAGCTCTTTAGCCAGTTTGAAGTCGACCTGTACC
  AAAATGAATCCGCGTGCCAGAGCCCACTGGATCATCAGTACCGCCAAGAGATCTTGAAACTGGAGAATTC
  TGGCGGCAGGAAAAACCGGCGAATCTTCACTTACACTGACTCGGATAGATACACCGACTTGGAAGAGTAC
  TGTCAGAAAATAACCACTTCAGCCAGCGAGGTGCCATCGTTTCTGGCTGAGCGGATGGCGAACGTCAGGC
  GGCGGCGGCAGGACAGGCGAGGGGTGGAGGGCAGCAAACTCAAGTCTCGGATCGTCACGTGGGAACCCTC
  GGAAGACTTCATCAAGAACAACCACGCTATTAACACTCCTCTTCAGACAATGTACATCATGGCGGACCTG
  GGACCCTATGGAAAGCTTGGCTATAAGGTACATGAGCACGTGCTCTGCATCCTGAAGGTGGACAGCAATG
  GCGTGATCACAGTGAAGCCTGACTTCACCGGCATCAAAGGACCCTACAGAATCGAAACTGAGGGAGAGAA
  GCAGGAACACACTTCTGCTTGGAAATACACAATTGACAACGTGTCCTCCCTCGCACAGCCAGAGGAGGAA
  GAGCGGGAGCAGCGAGTGTTCAAGGACCTTTACGGCCGGCACAAGGAGTACCTCAGCAGCCTCGTGGGCA
  CAGACTTTGAGATGATTGCCCCAGGTGCCCTCCGGCTCTTTGTGAACGGAGAGGTGGTTTCAGCCCAAGG
  CTATGAGTATGACAACCTCTACGTCCACTTCTTTGTGGAGCTGCCAGCTGCTAACTGGTCCAGCCCGCCA
  TTCCAGCAGCTCTCGGGAGTAACCCAGGCCTGTGCCACCAAATCCCTGGGAATGGACAAGGTGGCCTATT
  TCTCCTTTCCGTTCACATTCGAAGCCTTCTTTCTGCATGAGGACGAATCGGCTGAGTCCCTCCCGGAGTG
  GCCTGTGCTCTACTGTAAGGTCCTCTCACTGGACTTCTGGCAGAGGTATCGTGTGGAAGGCTACGGGGCT
  GTGGTGCTGCCTGCCACTCCAGGATCCCACACCCTGACTGTCTCCACATGGAGGCCCATGGAGCTGGGTC
  TCGTGGCAGAGCTAAGGAGGTTCTTCATTGGCGGCTCTCTGGAGCTGGAGGACCCATCCTATGTGAGGAT
  ACCAGGAACCTTCAAGGGGGAGCGCCTGAGCCGCTTTGGATTCCGCACTGAGACAACGGGTACTGTCACC
  TTCCGATTGCACTGCCTGCAGCAGTCCCGGGCTTTCATGGAATCGAACTCCCTCCAGAAACAGATGCGGA
  GTGTGTTGGACCGTCTGGAAGGGTTCAGCCAGCAGAGTTCCACGCACAACGTGCTAGAAGCATTCCGTCG
  AGCCCGGCGTCGGATGCAGGAGGCCCGAGAGAGCCTCCCCCAGGACCTTGTGAGCCCCACGGGAACGTTG
  ACCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001039684
• Insert Size: 1686 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039684.2, NP_001034773.2
• RefSeq Size: 2365 bp
• RefSeq ORF: 1686 bp
• Locus ID: 380718
• UniProt ID: Q5SW45
• Cytogenetics: 11 C
• Gene Summary: Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Involved in centrosome migration to the apical cell surface during early ciliogenesis. Required for ciliary structure and function, including a role in regulating length and appropriate number through modulating centrosome duplication. Required for cell branching morphology.[UniProtKB/Swiss-Prot Function]