Iffo1 (NM_178787) Mouse Untagged Clone

CAT#: MC219026

Iffo1 (untagged) - Mouse intermediate filament family orphan 1 (Iffo1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Iffo1
• Synonyms: 4733401N06Rik; A930037G23Rik; HOM-TES-103; Iffo
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219026 representing NM_178787
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATCCGTTATTTGGTCCCAACCTCTTCCTCCTACAGCAGGAGCAGCAAGGCTTGGCCGGGCCGCTGG
  GGGACCCTCTGGGAGGTGACCACTTTGCCGGGGGAGGGGACTTAGCCTCGGCTCCGCTTGCCTCGGCCGG
  CCCCTCTGCCTATTCGCCTCCGGGGCCTGGTCCGGCGCCCCCCGCAGCCATGGCTCTCCGCAACGACCTA
  GGCTCCAACATCAACGTACTGAAGACCCTGAACCTCCGGTTTCGCTGCTTCCTAGCCAAGGTGCACGAGC
  TAGAGCGCCGAAATCGGCTGCTGGAGAAACAGCTGCAGCAGGCTCTAGAGGAGGGTAAGCAGGGCAGGCG
  GGGCCTGGCCCGCCGTGACCAGGCGGTACAGACCGGCTTCATCAGCCCGATCCGGCCCCTGGGGCTGCCC
  CTGAGCTCCCGGCCGGCCGCCGTGTGCCCCCCATCAGCGCGGGTGCTGGGCTCGCCCTCCCGCTCGCCAG
  CTGGACCCTTAGCATCCTCTGCCGCCTGCCACACATCATCCTCCACTTCCACATCCACTGCCTTCTCCTC
  GTCGACCCGCTTCATGCCTGGCACCATCTGGTCCTTCTCTCACGCCCGCCGGCTTGGACCAGGACTGGAA
  CCCACGCTGGTGCAAGGGCCTGGCCTGTCGTGGGTACACCCTGACGGGGTGGGCGTCCAGATCGACACCA
  TCACCCCTGAGATCCGTGCCCTGTACAACGTGCTGGCCAAAGTGAAGCGGGAGCGGGACGAGTACAAGCG
  AAGGTGGGAAGAGGAATACACGGTTCGGATACAGCTACAAGAGCGAGTGACTGAGCTCCAGGAGGAGGCC
  CAAGAGGCCGATGCCTGCCAAGAGGAGCTAGCCATGAAGGTTGAGCAGCTGAAGGCAGAGCTGGTGGTCT
  TCAAGGGGCTCATGAGTAATAATCTGACAGAGCTGGACACCAAGATCCAGGAAAAGGCCATGAAAGTCGA
  CATGGACATCTGCCGCCGCATTGACATCACGGCCAAGCTCTGTGACTTGGCCCAGCAGCGCAACTGTGAG
  GATATGATCCAGATGTTCCAGGTCCCGTCCATGGGGGGGCGGAAGCGGGAGCGCAAGGCTGCTGTGGAGG
  AGGACACCTCCCTGTCGGAGAGTGATGGGCCCCGCCAGCCTGAGGGTGCCGAGGAGGAGAGCACAGCCCT
  CAGCATCAACGAGGAGATGCAGCGCATGCTCAGCCAGCTGAGGGAGTATGATTTTGAGGACGACTGTGAC
  AGCCTGACTTGGGAGGAGACTGAGGAGACCTTGCTACTTTGGGAGGATTTCTCAGGCTATGCCATGGCAG
  CCGCAGAGGCCCAGGGAGAGCAGGAGGACAGCCTGGAGAAGGTGATCAAAGACACGGAGTCCCTGTTCAA
  AACCCGGGAGAAGGAGTACCAGGAGACCATTGACCAGATAGAGCTGGAGCTGGCCACAGCCAAGAACGAC
  ATGAACCGACACCTGCATGAGTACATGGAGATGTGCAGCATGAAGCGGGGCCTGGACGTGCAGATGGAGA
  CCTGCCGCCGGCTCATCACACAGTCCGGGGACCGAAAGTCTCCTGCTTTCACTGCGGTCCCGCTTAGCGA
  CCCGCCGCCACCGCCGAGTGAGACTGAGGACTCCGATCGAGACGTCTCATCTGATAGCTCCATGAGATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178787
• Insert Size: 1680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178787.6, NP_848902.4
• RefSeq Size: 2857 bp
• RefSeq ORF: 1680 bp
• Locus ID: 320678
• UniProt ID: Q8BXL9
• Cytogenetics: 6 F2
• Gene Summary: Nuclear matrix protein involved in the immobilization of broken DNA ends and the suppression of chromosome translocation during DNA double-strand breaks (DSBs) (PubMed:31548606). Interacts with the nuclear lamina component LMNA, resulting in the formation of a nucleoskeleton that will relocalize to the DSB sites in a XRCC4-dependent manner and promote the immobilization of the broken ends, thereby preventing chromosome translocation (PubMed:31548606). Acts as a scaffold that allows the DNA repair protein XRCC4 and LMNA to assemble into a complex at the DSB sites (PubMed:31548606).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon in the coding region compared to variant 1. It encodes isoform b, which is shorter than isoform a.