Frmd3 (NM_001163732) Mouse Untagged Clone

CAT#: MC219022

Frmd3 (untagged) - Mouse FERM domain containing 3 (Frmd3), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Frmd3
• Synonyms: 4.1O; 9430066I12Rik; EPB41L4O; P410
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219022 representing NM_001163732
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCGCTTCTTGTCACTGTGCGCCGAGAGGCAGAAGGACTATGAAGATGATTCACTTTCGGAGCTCCA
  GTATCAAATCGCTCAACCAGGAGATGAAATGCACCATTCGACTGCTGGACGACTCGGAGGTCTCCTGCCA
  TATCCAGAGGGAAACGAAAGGGCAGTTTCTCATTGAATACATCTGCAACTACTATAGCCTGCTGGAAAAG
  GACTACTTTGGAATTCGCTATGTGGACCCAGAGAAGCAAAGGCACTGGCTTGAGCCTAACAAGTCCATCT
  TCAAGCAAATGAAATCTCATCCACCATACACCATGTGCTTTAGAGTGAAATTCTACCCACATGAACCCTT
  GAAGATTAAAGAAGAGCTCACAAGGTACCTATTATATTTGCAAATTAAAAGAGACATTTTTCATGGTCGA
  CTGCTGTGTTCCTTTTCTGATGCTGCCTATCTGGGTGCTTGTATCGTTCAAGCTGAGTTTGGTGATTACT
  ATCCTGATGAGCATCCTGAGAATTATATCAGTGAGTTTGAGATTTTTCCCAAGCAGTCACAGAAATTGGA
  AAGAAAAATCATGGAAATTCATAACAACGAACTAAGAGGTCAGAGCCCAGCAATTGCTGAATTCAACTTA
  CTCTTAAAAGCTCATACATTGGAAACTTATGGAGTGGATCCTCACCCATGCAAGGATTCAAGAGGTGCTA
  CAGCATTTTTAGGATTCACTGCTGCAGGTTTCGTCGTATTCCAGGGAAATAAAAGAATCCATTTGAGAAA
  ATGGTCAGATGTCTGCAAATTGAAGTTTGAAGGGAAGACATTTTACGTGATTGGTTCCCAGAAAGAGAAA
  AATGCTGTTTTGGCATTCCATACATCAACACCAGCTGCCTGCAAACATTTATGGAAATGTGGGGTAGAAA
  ACCAAGCCTTTTATAAGTATGCAAAGTCCAGTCAGATCAAGACTGTGTCAAGCAGCAAGATATTTTTTAA
  AGGAAGTAGATTTCGATACAGTGGTAAAGTTGCTAAAGAGGTGGTAGAGGCAAGCTCCAAAATCCAGAGG
  GATCCTCCTGAAGTGCATAGAGTCAACATTACTCAGAGTCGCAGCTTTCACTCCTTGAACAAACAGCTCA
  TCATCAACATGGAACCTTTGCAGCCCCTGCTTCCTTCCCCCACTGAACAGGAAGAAGAAGTTCCTGTGGG
  TGAGGGTGTCCCACTGCCTAAAATGGATGTGTCTGAGCCCCTAATTGCCAGCTCACCAGTGAAGGGAGCA
  CAGTGTGCAGATCCTCCAGATGAAGAGGAAGATAGAGTAAAAGAAGACCCCTTGACCATCTCTGAACTAG
  CGTACAATCCCAGCGCCAGCCTGCTTCCTACCCCAGTGGATGATGATGAAATCAACATGCTCTTTGACTG
  TCCATCCAGGCTTGAGTTAGAAAGAGAAGACACGGATTCATTTGAGGAACTTGAAGCAGATGAAAATGCT
  TTTTTGATTGCTGAAGAGGAGGAGCTGAAGGAAGCTCGCCAAGCTTTGTCATGGAGCTATTCAATCCTGA
  CCGGCCATATCTGGGTCAACCCCCTGGTCAAGAGTTTCTCCAGGCTCCTTGTGGTGGGCCTGGGACTGCT
  GCTGTTTGTATTTCCCCTGCTCCTCCTCCTTTTGGAGTCAGTCTCTATGCCATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001163732
• Insert Size: 1665 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163732.1, NP_001157204.1
• RefSeq Size: 2044 bp
• RefSeq ORF: 1665 bp
• Locus ID: 242506
• UniProt ID: Q8BHD4
• Cytogenetics: 4 C3
• Gene Summary: Putative tumor suppressor gene that may be implicated in the origin and progression of lung cancer.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses a different exon for its 3' terminus, compared to variant 1, which results in a protein (isoform 2) with a shorter and distinct C-terminus, compared to isoform 1.