Pigz (NM_172822) Mouse Untagged Clone

CAT#: MC219020

Pigz (untagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class Z (Pigz), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pigz
• Synonyms: F630022B06Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219020 representing NM_172822
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGTGGCAGCCCAGGTGATTTGGGGTAGCTTCAGCCTGCTTCGTGTCTTATGGTGTCTCCTTCCCC
  AGACTGGCTACATACACCCAGATGAATTCTTCCAGTCACCTGAGGTCATGGCAGAGGACATCCTGGGTGT
  GCAGGCTACACGGCCCTGGGAATTTTACCCCAGCAGCTCCTGCCGCACTGTGGTCTTCCCACTGCTGACC
  TCTGGCTCTACCTTCTGGTTACTCAGGCTCTGGGAAGAGCTGGGGCTCTGGCCTGGTCTGGTGAGTGGCT
  ACATGCTGCTGGTGGGGCCCCGATTCCTTCTCACCGCCCTCTCCTTTGCCCTGGACTGGGCTGTGTACGA
  TCTGGCCCCGTTGTGGGGGGCAGATCGTTGGAATGCCTTGTGCCTGCTGTCTGGTTCCTATGTCACCCTG
  GTTTTCTACACAAGAACCTTCTCCAACACCATCGAGGGACTGCTCTTCACCTGGCTGCTGGTGCTGGTGT
  CCCCTGGTGTGGTAAGGAGTCCTACATCAAAGAAGCCTACCCCAGGTCCACGGTGGCACAGATATCTTCT
  TGGTGTTATCTTGGCTGCTGGCTTCTTCAACCGGCCAACCTTTCTAGCCTTTGCTCTGGCCCCCCTCTCT
  CTCTGGGGCATCCATAGAGCTTCAGAACTTGGTGGCATCAGGGCCCTGATCCAGGAGGCCCTGGTGCTGT
  TGCCAGGGGCTGCTCTTGCTGCAGTGCTGTGTGTAGCCACGGACAGCTGGTACTTCTCCAGCCTCTCGAG
  ATCCACAGGCGTCTTTCTCACACCTGCCAACTTCTTGTACTACAACCTGGACCCCCAAAACCTTGCCAGG
  CACGGCACACACGCACGGCTCACTCACTTGGCAGTCAATGGCTTCCTGCTCTTTGGGGTGTTGCATGCTC
  AAGCTCTGCAGGCTGCATGGCAACAACTCCACGCCTGCCTCCGTGCATCCCCACAAACGGGTCTCTCCAG
  GGTGCGGGGTGCCCGGGGTCTGTTGTCTAGCTCCAAGTCTTATCTCCTCCTCTTCTACTTTACACCCCTG
  CTCCTGCTTTCTGCCTTCAGCCATCAAGAGGCTAGATTTCTGATTCCCCTCCTAGTCCCTCTAGTCCTGC
  TGTGCAGTCCACAAACCCAACCTATACCCTGGAAAGGCACCCTGGTCCTCTTCAACATCCTGGGCGCTCT
  CATCTTTGGTTGCCTGCATCAGGGAGGTCTGGTACCGGGCCTGAAGTACCTGGAGCAGATAGTCCACACG
  CCTGACCTCTCAGGCACGACCACCCACTACACACTTCTCTTCACACACACCTACATGCCTCCCCAGCACC
  TCTTACACCTCTCAGGCCTGGGATCACCCGTGGAGGTGGTAGACATGGGTGGGGCTGAGGACAGGGTCCT
  GTGTCAAGCCCTGAACAACTTCAGCAGACAACCTGCCTGCCAACTGGCTGGTGAGCCATGGCCCTGTCGG
  CTCTTTGTGGTAACTCCTGGGACCAACAGGCACGCTCTGGAGAAGTGCCGTTTTCCCCTCAAGAATGAGA
  CTCTCTTGTTTCCCCACTTGACCCTGGAGGACCCTCCAGCCCTGTCCTCTCTGCTCAGTGGGGCTTGGAG
  GAAGCACCTTAGTCTCCATGTCATAGAACTGGAGACGCCTGTTGTGACAAAGCAGCCCAAGGCTCAGCCA
  TAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172822
• Insert Size: 1683 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172822.3, NP_766410.2
• RefSeq Size: 2184 bp
• RefSeq ORF: 1683 bp
• Locus ID: 239827
• UniProt ID: Q8BTP0
• Cytogenetics: 16 B2
• Gene Summary: Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers a fourth mannose to some trimannosyl-GPIs during GPI precursor assembly. The presence of a fourth mannose in GPI is facultative and only scarcely detected, suggesting that it only exists in some tissues (By similarity).[UniProtKB/Swiss-Prot Function]