Ric8b (NM_183172) Mouse Untagged Clone

CAT#: MC219019

Ric8b (untagged) - Mouse resistance to inhibitors of cholinesterase 8 homolog B (C. elegans) (Ric8b), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ric8b
• Synonyms: BC051080; Ric-8; Ric-8b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219019 representing NM_183172
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAAGAGCGCGCCCTCTACATCGTCCGGGCCGGCGAGGCGGGGGCTATCGAGCGGGTCCTGAGGG
  ATTACAGCGACAAGCATAGGGCTACTTTCAAATTTGAATCAGCAGATGAAGATAAAAGAAAGAAACTCTG
  TGAAGGCATATTTAAGGTCCTTGTCAAGGAGGTCCCAACGACATGCCAAGTGTCCTGCCTGGAAGTACTC
  CGCATTCTCTCCAGAGACAAAAAGATCTTAGTTCCTGTAACAACTAAGGAAAATATGCAAATACTGCTGA
  GACTAGCCAAGCTCCACGAGTCAGACGATTCTTTGGAGAAGGTGTCAGAGTTCCCAGTTATTGTGGAATC
  ATTGAAGTGTCTGTGTAACATCGTGTTCAACAGTCAGATGGCACAGCAGCTTAGCCTGGAACTGAATCTC
  GCTGCAAAGCTCTGTAACCTCCTAAGGAAGTGCAAGGACCGAAAATTTATCAATGACATAAAGTGCTTTG
  ACTTGCGCTTGCTCTTCGTCTTGTCACTTTTGCACACTGACATCAGGTCACAATTGCGCTATGAGCTCCA
  GGGACTCCCGCTGCTAACCCAGATCTTGGAAAGTGCCTTTAGCATCAAGTGGACCGATGAGTATGAGTCG
  GCCATAGACCATAATGGACCTCCTCTCTCACCTCAGGAGACAGACTGTGCCATTGAGGCCCTCAAAGCTC
  TGTTCAATGTGACGGTAGACAGTTGGAAGGTGCATAAAGAGAGCGATTCTCATCAGTTTCGTGTCATGGC
  AGCTGTCCTTCGCCATTGTTTACTAATTGTAGGTCCAACTGAAGACAAAACCGAAGAGCTGCACAGTAAT
  GCCGTCAACCTTTTAAGCAATGTTCCAGTCTCTTGTTTGGATGTTCTCATTTGTCCATTAACCCATGAAG
  AAACAGCCCAAGAGGCAGCAACTCTAGATGAACTGCCCAGTGATAAAACAACGGAGAAAGACACAGCTTT
  GAAAAACAGTACCATGGTATACAATGGCATGAATATGGAGGCCATTCACGTTTTACTCAATTTTATGGAG
  AAGAGAATTGATAAGGGAAGCAGCTATAGAGAGGGTCTAACTCCAGTTCTCAGCTTATTAACAGAATGTT
  CCCGAGCCCATCGGAACATCCGAAAATTTCTCAAAGATCAGGTTTTACCGCCTTTGAGAGATGTGACAAA
  TCGACCAGAAGTCGGCTCAACCGTGAGAAATAAGCTGGTTCGTCTCATGACACATGTTGACCTCGGAGTC
  AAGCAGATTGCTGCTGAGTTCCTTTTTGTCCTTTGCAAAGAGAGAGTGGATAGCCTGCTGAAATACACTG
  GCTATGGGAACGCTGCAGGACTCTTGGCGGCCAGGGGCCTCTTGGCTGGAGGAAGAGGAGATAATTGGTA
  CTCAGAGGACGAGGACACGGACACTGAAGAATACAAAAACGCAAAACCAAACATTAATCTTATCACTGGT
  CATTTAGAGGAACCAATGCCAAACCCTATAGATGAAATGACAGAAGAACAAAAAGAATATGAAGCCATGA
  AACTTGTCAACATGCTTGATAAACTTTCCAGAGAGGAGTTGCTTAAACCAATGGGACTAAAACCTGACGG
  GACAATAACGCCTTTGGAGGAAGCACTCAGCCAGTATTCTGTCATCGAAGAGACCAGCTCGGACACAGAC
  TGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_183172
• Insert Size: 1683 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183172.2, NP_898995.1
• RefSeq Size: 5017 bp
• RefSeq ORF: 1683 bp
• Locus ID: 237422
• UniProt ID: Q80XE1
• Cytogenetics: 10 C1
• Gene Summary: Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP (By similarity). Able to potentiate G(olf)-alpha-dependent cAMP accumulation suggesting that it may be an important component for odorant signal transduction.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate in-frame exon in the coding region compared to variant 1. It encodes isoform 2, which is longer than isoform 1.