Traf3ip2 (NM_134000) Mouse Untagged Clone

CAT#: MC218993

Traf3ip2 (untagged) - Mouse TRAF3 interacting protein 2 (Traf3ip2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Traf3ip2
• Synonyms: Act1; AI429613; CIKS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218993 representing NM_134000
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCGAAGCATTCCCGTGGAGGTTGATGAATCAGAACCATTCCCGAGTCAATTGCTGAAACCGATCC
  CAGAATACTCTCCAGAAGAGGAATTGGAACCACCTGCCCCCAATACAAGGAACATGGCACCCAGCAGCTT
  GTCTGTGCTCCAGTGCCCTCCCCTGAAACTGGCAAACCACCAGCCTGTGTCACAACAGGTCACCTGCCTG
  CGAGCTAAAGTCCTGGAGGAAGGTGAAGCCAGTTTCTTTAGAAGGCACCCTGAACTTGGCAAAGATATTT
  CCTCATGTTCCTCTGGAGCCAGCGAGCCTGAGTCTGAGCTCGGAGCCCTCCCTCCAGAGCATCGGTTCAC
  ACTTACGGAAAAACGCAATAGATGGCTGGGATCTCAGCTTTCAGCAGCTTCTCCTGACACTGGCCATGAA
  TCAGACAAATCAGACCCAAGTTTACCTAATGCTTTGGCAGACTCCTTCAGTGGTGGCCAAGAGATGATGC
  CCCGGCCCCGGCCCCGGCCTGGGCCCCACAGGCACAGGGCAGCCCCAGATGTGCCCACGATAGACACTGG
  CTATGATTCCCAGCCCCAAGATGTCCTGGGCATCAGGCAGTTGGAGAGGCCATTGCCCCTCACCTCCTCG
  TGTTACCTGCAGGACCTCCCTGGGCCTCTGAGGTCCAGGGAATTGCCACCTCAGTTTGAACTTGAGAGGT
  ATCCAATGAACGCCCAGCTGCTGCCGCCCCATCCTTCCCCACAGGCCCCATGGAACTGTCAGTACTACTG
  CCCCGGAGGGCCCTACCACCACCAGGTGCCACACGGCCATGGCTACCCTCCAGCAGCAGCCTACCAGCAA
  GTACTCCAGCCTGCTCTGCCTGGGCAGGTCCTTCCTGGGGCAAGGGCAAGAGGCCCACGCCCTGTGCAGA
  AGGTCATCCTGAATGACTCCAGCCCCCAAGACCAAGAAGAGAGACCTGCACAGAGAGACTTCTCTTTCCC
  GAGGCTCCCGAGGGACCAGCTCTACCGCCCACCATCTAATGGAGTGGAAGCCCCTGAGGAGTCCTTGGAC
  CTTCCTGCAGAGCTGAGACCACATGGTCCCCAGGCTCCATCCCTAGCTGCCGTGCCTAGACCCCCTAGCA
  ACCCCTTAGCCCGAGGAACTCTAAGAACCAGCAATTTGCCAGAAGAATTACGGAAAGTCTTTATCACTTA
  TTCTATGGACACAGCCATGGAGGTGGTGAAATTTGTGAACTTTCTGTTGGTGAACGGCTTCCAAACTGCG
  ATTGACATATTTGAGGATAGAATCCGGGGTATTGATATCATTAAATGGATGGAGCGCTATCTTCGAGATA
  AGACAGTGATGATAATCGTAGCAATCAGCCCCAAATACAAACAGGATGTGGAAGGCGCTGAGTCGCAGCT
  GGACGAGGACGAGCATGGCTTACATACTAAGTACATTCATCGGATGATGCAGATTGAGTTCATAAGTCAG
  GGAAGCATGAACTTCAGATTCATCCCTGTGCTCTTCCCAAATGCCAAGAAGGAGCATGTGCCGACCTGGC
  TTCAGAACACTCATGTTTACAGCTGGCCCAAGAATAAGAAAAACATCCTGCTGCGGCTGCTCAGGGAGGA
  AGAGTATGTGGCTCCTCCCCGAGGCCCTCTGCCCACCCTTCAGGTGGTACCCTTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_134000
• Insert Size: 1668 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC096483, AAH96483
• RefSeq Size: 2867 bp
• RefSeq ORF: 1668 bp
• Locus ID: 103213
• UniProt ID: Q8N7N6
• Cytogenetics: 10 B1
• Gene Summary: E3 ubiquitin ligase that catalyzes 'Lys63'-linked polyubiquitination of target protein, enhancing protein-protein interaction and cell signaling (By similarity). Transfers ubiquitin from E2 ubiquitin-conjugating enzyme UBE2V1-UBE2N to substrate protein (By similarity). Essential adapter molecule in IL17A-mediated signaling (PubMed:19825828). Upon IL17A stimulation, interacts with IL17RA and IL17RC receptor chains through SEFIR domains and catalyzes 'Lys63'-linked polyubiquitination of TRAF6, leading to TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways (PubMed:19825828).[UniProtKB/Swiss-Prot Function]