Tcp1 (NM_013686) Mouse Untagged Clone

CAT#: MC218964

Tcp1 (untagged) - Mouse t-complex protein 1 (Tcp1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Tcp1
• Synonyms: AI528772; c-cpn; CCT; Cct1; Ccta; p63; Tcp-1; Tp63; TRic
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218964 representing NM_013686
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGTCCTTTGTCCGTGTTCGGGGACCGCAGCACTGGGGAGGCGGTCCGCTCCCAGAATGTTATGG
  CTGCAGCTTCTATTGCCAACATTGTTAAAAGTTCTTTTGGGCCAGTTGGCTTGGATAAAATGTTGGTGGA
  TGATATTGGTGATGTAACCATTACTAACGATGGTGCCACCATCCTGAAGTTACTGGAGGTAGAACATCCC
  GCAGCCAAAGTTCTGTGTGAGCTGGCTGACCTGCAAGACAAAGAAGTTGGAGATGGAACTACCTCAGTGG
  TAATCATTGCAGCGGAGCTTCTGAAAAATGCAGATGAGCTAGTCAAACAGAAAATTCATCCAACATCAGT
  TATTAGTGGCTATCGTCTTGCCTGCAAGGAAGCGGTGCGTTATATCAATGAGAACCTGATTATTAACACA
  GACGAACTTGGAAGAGACTGTCTGATCAATGCTGCTAAGACATCCATGTCATCCAAAATTATTGGAATAA
  ATGGTGATTACTTTGCTAATATGGTAGTAGATGCTGTGCTTGCTGTTAAATACACAGATGCCAGAGGCCA
  GCCTCGCTATCCAGTCAATTCTGTTAATATTCTGAAAGCCCATGGGAGAAGTCAGATAGAAAGCATGCTG
  ATCAATGGCTATGCGCTCAATTGTGTGGTTGGATCTCAGGGCATGCCCAAGAGAATAGTTAATGCAAAAA
  TTGCTTGTCTTGACTTCAGCCTGCAGAAAACAAAAATGAAGCTTGGTGTACAGGTGGTTATTACAGACCC
  TGAGAAATTGGACCAAATTAGACAGAGAGAATCGGATATCACCAAGGAGAGAATTCAGAAGATCCTGGCA
  ACTGGTGCCAATGTTATTCTAACCACTGGTGGCATTGATGATATGTATCTCAAGTATTTTGTGGAAGCTG
  GTGCCATGGCTGTTAGGAGAGTTTTAAAACGAGACCTGAAGCATGTTGCAAAAGCTTCTGGAGCAAGTAT
  CCTGTCTACGCTGGCCAATTTGGAAGGCGAAGAAACTTTTGAAGTGACGATGTTGGGACAAGCGGAAGAG
  GTCGTACAGGAGAGAATTTGTGATGATGAGCTGATCTTAATCAAAAATACTAAGGCTCGTACATCTGCTT
  CAATCATCTTACGAGGAGCAAATGATTTCATGTGTGATGAAATGGAGCGCTCTTTACATGATGCTCTTTG
  TGTGGTGAAGAGAGTTTTGGAGTTGAAATCTGTGGTCCCAGGTGGAGGTGCTGTAGAAGCTGCCCTGTCC
  ATATACCTGGAAAACTATGCAACAAGCATGGGATCTCGGGAACAGCTTGCTATTGCAGAGTTTGCAAGAT
  CTCTGCTTGTGATTCCTAATACACTGGCAGTGAATGCTGCCCAGGACTCCACCGACCTGGTTGCCAAGTT
  AAGAGCTTTTCACAATGAGGCTCAAGTGAACCCGGAACGTAAAAATCTAAAGTGGATTGGTCTTGATTTG
  GTCCATGGGAAACCACGAGACAACAAGCAAGCAGGGGTGTTTGAACCAACCATAGTTAAAGTGAAGAGCC
  TGAAGTTCGCAACAGAGGCTGCAATCACCATCCTTCGGATTGATGATCTGATAAAATTACACCCAGAAAG
  CAAAGACGATAAACACGGAAGTTATGAAAATGCTGTTCACTCTGGAGCCCTTGATGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013686
• Insert Size: 1671 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013686.4, NP_038714.2
• RefSeq Size: 2499 bp
• RefSeq ORF: 1671 bp
• Locus ID: 21454
• UniProt ID: P11983
• Cytogenetics: 17 8.72 cM
• Gene Summary: Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).