Slc1a6 (NM_009200) Mouse Untagged Clone

CAT#: MC218961

Slc1a6 (untagged) - Mouse solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6 (Slc1a6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slc1a6
• Synonyms: EAAT4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218961 representing NM_009200
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAGCCACGGCAATAGTCTGTTCCTGAGGGAGAGCGGTGCCGGTGGGGGCTGTCTGCAGGGCCTGC
  AGGACAGCCTGCAGCAGAGAGCACTGCGCACGCGCTTGCGCCTGCAGACCATGACCCGAGAGCACGTGAG
  GCGCTTCCTGCGCCGGAATGCCTTCATCTTGCTCACAGTCAGCGCTGTGATCATTGGTGTCAGCTTGGCA
  TTTGCCTTGCGCCCGTACCAGCTCTCCTACCGCCAGATCAAGTACTTCTCCTTTCCTGGTGAGCTGCTCA
  TGAGGATGCTGCAGATGCTGGTGTTACCCCTCATTGTCTCCAGCCTTGTCACAGGTATGGCATCCCTGGA
  CAACAAGGCGACAGGGCGGATGGGAATGCGGGCAGCTGTGTACTACATGGTGACTACAGTCATTGCAGTT
  TTCATCGGCATCCTAATGGTTACCATCATACATCCTGGGAAGGGCTCCAAGGAGGGGCTGCACCGTGAGG
  GCCGAATTGAGACTGTCCCAACAGCTGATGCTTTCATGGACCTGGTCAGAAATATGTTTCCACCTAACCT
  TGTGGAGGCCTGCTTCAAACAGTTTAAGACACAGTACAGCACACGAGTGGTGACAAGGACAATTGTAAGG
  ACAGACAATGGTTCAGAGCTGGGGGCCTCCATGTCTCCCACATCCTCAGTAGAAAATGAAACCAGCATCC
  TGGAAAATGTCACCCGGGCCTTGGGCACCCTGCAGGAGGTGATAAGCTTTGAGGAGACTGTGCCTGTACC
  TGGCTCAGCCAATGGCATCAATGCCTTGGGCCTTGTGGTCTTCTCTGTGGCCTTTGGGCTGGTCATTGGT
  GGCATGAAGCACAAAGGCCGTGTCCTGAGGGACTTCTTTGACAGCCTCAATGAGGCTATTATGAGGCTGG
  TGGGCATCATCATCTGGTATGCACCAGTGGGCATCCTGTTCCTGATTGCTGGAAAGATTCTGGAAATGGA
  AGACATGGCTGTCCTTGGAGGTCAGCTGGGCATGTACACACTGACTGTCATCGTTGGCTTATTCCTTCAT
  GCTGGTGGTGTGTTGCCCCTTATCTACTTCCTCGTCACCCATCGGAACCCCTTTCCCTTCATTGGTGGTA
  TGCTACAGGCACTCATCACAGCTATGGGTACCTCTTCCAGCTCTGCAACTCTGCCTATCACTTTCCGCTG
  CCTGGAGGAGGGCCTGGGTGTGGATCGTCGCATCACCAGGTTCGTGCTGCCTGTGGGGGCCACTGTCAAC
  ATGGATGGCACTGCACTCTACGAGGCCTTGGCAGCCATCTTTATTGCTCAAGTCAACAACTATGAGCTCA
  ACCTTGGTCAGATCACCACAATCAGCATCACAGCCACAGCTGCCAGCGTAGGAGCAGCTGGCATCCCCCA
  GGCAGGACTGGTTACCATGGTGATTGTGCTCACATCCGTCGGCCTGCCCACAGAGGACATCACACTGATC
  ATAGCTGTGGATTGGTTCCTTGACCGACTTCGTACAATGACCAATGTACTGGGGGATTCGATTGGAGCGG
  CTGTCATTGAGCATTTGTCCCAACGGGAGCTGGAGCTGCAGGAGGCTGAGCTGACTCTACCCAGCCTGGG
  GAAACCCTATAAGTCACTCATGGCACAAGAAAAGGGGGCATCAAGGGGTCGGGGAGGTAATGAGAGCGTC
  ATGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009200
• Insert Size: 1686 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009200.3, NP_033226.1
• RefSeq Size: 2089 bp
• RefSeq ORF: 1686 bp
• Locus ID: 20513
• UniProt ID: O35544
• Cytogenetics: 10 39.72 cM
• Gene Summary: Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:9379843). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (By similarity). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (Probable).[UniProtKB/Swiss-Prot Function]