Med1 (NM_134027) Mouse Untagged Clone

CAT#: MC218953

Med1 (untagged) - Mouse mediator complex subunit 1 (Med1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Med1
• Synonyms: AI480703; CRSP210; DRIP205; l11Jus15; PBP; Pparbp; TRAP220
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218953 representing NM_134027
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGCTCAGGGGGAAACCGAGGACTCAGAGAGGCTGAGTAAGATGAGCTCCCTCCTGGAACGGCTCC
  ATGCAAAATTTAACCAGAACAGACCTTGGAGTGAAACCATTAAGCTTGTGCGTCAAGTAATGGAGAAGAG
  GGTCGTAATGAGTTCTGGAGGGCATCAGCATTTGGTCAGCTGTTTGGAGACATTGCAGAAGGCTCTCAAA
  GTAACATCTTTGCCAGCAATGACTGATCGTTTGGAATCTATAGCCAGACAGAATGGACTGGGCTCTCACC
  TCAGTGCCAGTGGCACTGAGTGTTACATCACGTCAGATATGTTCTATGTGGAAGTGCAGTTAGATCCTGC
  AGGACAGCTTTGTGATGTCAAAGTGGCTCACCATGGGGAGAATCCTGTGAGCTGTCCAGAGCTTGTACAG
  CAGTTAAGGGAAAAGAATTTTGAGGAATTTTCCAAGCATCTTAAGGGTCTTGTTAATCTGTATAATCTCC
  CAGGGGACAACAAACTGAAGACTAAAATGTATCTGGCTCTCCAATCCTTAGAACAGGACCTTTCTAAAAT
  GGCTATTATGTACTGGAAGGCAACCAACGCCGCTCCCTTGGATAAGATTCTTCATGGAAGTGTTGGTTAT
  CTCACCCCGCGGAGTGGGGGTCATTTAATGAATATGAAATACTATGCCTCTCCATCTGACCTGCTGGATG
  ATAAGACTGCCTCTCCTATCATTTTGCATGAAAAGAATGTTCCTCGGTCTTTGGGAATGAATGCCTCAGT
  GACAATTGAAGGAACCTCTGCTATGTACAAACTCCCAATTGCCCCATTAATTATGGGGTCACACCCAGCT
  GACAACAAATGGACCCCTTCTTTCTCCGCAGTCACTAGTGCCAACAGTGTTGATCTTCCTGCGTGTTTCT
  TCTTGAAATTTCCCCAGCCAATTCCAGTATCTAAAGCATTTGTTCAGAAACTGCAAAATTGCACAGGAAT
  CCCGTTGTTTGAGACTCCGCCCACTTACCTGCCCCTGTATGAACTCATCACTCAGTTTGAGCTGTCAAAG
  GATCCTGACCCTTTACCTTTGAATCACAACATGCGATTTTACGCTGCTCTTCCAGGTCAGCAGCACTGCT
  ATTTTCTCAATAAAGATGCTCCTCTTCCTGATGGTCAGAGCCTGCAGGGAACACTGGTCAGCAAAATCAC
  CTTCCAGCACCCTGGCCGAGTTCCTCTTATCTTGAATATGATCAGACACCAAGTGGCCTATAACACTCTA
  ATTGGAAGCTGTGTCAAAAGAACTATTTTAAAAGAAGATTCTCCTGGGCTCCTCCAGTTTGAAGTGTGTC
  CTCTCTCAGAATCTCGCTTCAGTGTATCTTTTCAGCACCCTGTGAATGACTCCCTTGTGTGTGTGGTGAT
  GGATGTGCAAGACTCAACACATGTGAGCTGTAAACTCTACAAGGGGCTGTCAGATGCACTAATCTGTACA
  GACGACTTCATTGCCAAAGTTGTTCAAAGATGTATGTCCATTCCTGTGACGATGAGGGCTATTCGGAGGA
  AGGCTGAAACCATACAGGCTGACACCCCAGCACTGTCTCTCATTGCAGAGACAGTTGAAGACATGGTGAA
  AAAGAACCTGCCCCCGGCTAGCAGCCCAGGATCAAAGAATCCCGAGTTAGGATCTGGATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_134027
• Insert Size: 1671 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134027.2, NP_598788.2
• RefSeq Size: 2822 bp
• RefSeq ORF: 1671 bp
• Locus ID: 19014
• UniProt ID: Q925J9
• Cytogenetics: 11 61.75 cM
• Gene Summary: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Essential for embryogenesis, including development of the central nervous system, heart, liver and placenta and for erythropoiesis. Also required for normal transcriptional control of thyroid-stimulating hormone beta (TSHB) in the pituitary. Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (By similarity).[UniProtKB/Swiss-Prot Function]