Has3 (NM_008217) Mouse Untagged Clone

CAT#: MC218946

Has3 (untagged) - Mouse hyaluronan synthase 3 (Has3), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Has3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218946 representing NM_008217
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGTGCAGCTGACTACAGCCCTGCGTGTGGTGGGCACCAGTCTGTTTGCCCTGGTAGTGCTGGGAG
  GCATCCTGGCGGCCTATGTGACAGGCTACCAGTTTATCCACACAGAAAAGCACTACCTGTCCTTTGGCCT
  CTACGGTGCCATCCTGGGTCTACATCTGCTCATCCAGAGCCTGTTTGCCTTCCTGGAGCACCGTCGAATG
  CGCAGGGCAGGGCGCCCCCTCAAGCTGCACTGCTCCCAGAGGCCGCGTTCAGTGGCACTCTGCATTGCTG
  CCTACCAAGAGGACCCCGAATACCTGCGCAAGTGCCTTCGCTCAGCTCAGCGCATTGCCTTTCCAAACCT
  CAAGGTGGTCATGGTAGTGGATGGCAATCGCCAGGAAGATACCTACATGTTGGACATCTTCCATGAGGTG
  CTGGGTGGCACTGAGCAAGCTGGCTTCTTTGTGTGGCGTAGCAATTTCCATGAGGCGGGTGAAGGAGAGA
  CAGAGGCCAGCCTGCAGGAAGGCATGGAGCGTGTGCGAGCTGTGGTGTGGGCCAGCACCTTCTCATGCAT
  CATGCAGAAGTGGGGGGGCAAGCGTGAGGTCATGTACACTGCCTTCAAGGCCCTTGGCAACTCAGTGGAC
  TACATCCAGGTGTGTGACTCTGACACTGTGCTGGACCCAGCCTGCACCATTGAGATGCTTCGAGTCTTGG
  AAGAAGATCCCCAAGTAGGAGGTGTTGGAGGAGATGTCCAAATCCTCAACAAGTATGATTCATGGATCTC
  CTTCCTGAGCAGTGTGAGGTACTGGATGGCTTTCAACGTGGAGCGGGCCTGCCAGTCCTACTTTGGCTGT
  GTGCAATGTATTAGTGGGCCTTTGGGCATGTACCGCAACAGCCTCCTTCAGCAGTTCCTGGAGGATTGGT
  ACCATCAGAAGTTCCTAGGCAGCAAGTGCAGCTTTGGGGATGATCGGCACCTTACCAACCGAGTCCTGAG
  TCTTGGCTACCGGACTAAGTATACAGCACGCTCTAAGTGCCTCACAGAGACCCCCACTAGGTACCTTCGA
  TGGCTCAATCAGCAAACCCGCTGGAGCAAGTCTTACTTTCGGGAATGGCTCTACAATTCTCTGTGGTTCC
  ATAAGCACCACCTCTGGATGACCTATGAATCAGTGGTCACAGGTTTCTTCCCATTCTTCCTCATTGCTAC
  AGTCATACAACTTTTCTACCGTGGCCGCATCTGGAACATTCTCCTCTTCCTGCTAACAGTGCAGCTGGTG
  GGCATTATCAAGGCTACCTATGCCTGCTTCCTTCGAGGCAATGCAGAGATGATCTTCATGTCCCTCTACT
  CCCTTCTCTATATGTCCAGCCTCTTGCCAGCCAAGATCTTTGCTATTGCTACCATCAACAAGTCTGGCTG
  GGGCACTTCTGGCAGGAAAACCATTGTCGTGAACTTCATTGGCCTAATCCCCGTGTCCATCTGGGTGGCA
  GTTCTTCTAGGGGGGTTAGCCTACACAGCTTATTGCCAGGACCTGTTCAGTGAGACCGAGCTAGCCTTCC
  TAGTCTCTGGGGCCATCCTGTATGGCTGCTACTGGGTGGCCCTCCTCATGCTGTATCTGGCCATTATTGC
  CCGGAGGTGTGGGAAGAAGCCAGAACAGTATAGCCTGGCTTTTGCGGAGGTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008217
• Insert Size: 1665 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008217.4, NP_032243.2
• RefSeq Size: 5912 bp
• RefSeq ORF: 1665 bp
• Locus ID: 15118
• UniProt ID: O08650
• Cytogenetics: 8 53.28 cM
• Gene Summary: Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.