Cbfa2t3 (NM_177289) Mouse Untagged Clone

CAT#: MC218937

Cbfa2t3 (untagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) (Cbfa2t3), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cbfa2t3
• Synonyms: A630044F12Rik; AI465270; AW229127; Cbfa2t3h; ETO-2; Eto2; MTGR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218937 representing NM_177289
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGACTCCCCGGCTGAAGTGAAGACGCAGCCCCGGTCCACACCCCCCAGCATGCCGCCCCCACCTC
  CCACCTCATCCCAGGGAGCCACACGACCACCCTCCTTCACACCTCACACACTGATGAACGGCAGCAGCCA
  CTCACCCACGGCCATCCACGGTGCCCCATCTACACCCAATGGCTTCAGCAACGGCCCAGCCACCTCATCC
  ACGGCCTCGCTCTCCACACAGCACCTGCCCCCGGCGTGCGGGGCACGGCAGCTCAGCAAGCTTAAGCGTT
  TCCTCACCACCCTACAGCAGTTTGGCAGTGACATCTCACCTGAGATCGGGGAGCGCGTGCGCACACTGGT
  GCTGGGCCTGGTGAACTCAACTCTGACGATCGAAGAGTTTCATGCCAAGCTCCAGGAAGCCACCAACTTT
  CCACTGAGGCCGTTTGTTATCCCTTTTCTGAAGGCTAATCTTCCACTGCTGCAGCGTGAGCTCCTGCACT
  GTGCCCGCCTGGCCAAACAGACACCTGCCCAGTACCTGGCCCAGCACGAACAGCTGCTGCTGGACGCCAG
  CGCCACCTCCCCTGTCGACTCGTCTGAGCTCCTGCTGGAAGTCAACGAGAACGGCAAAAGGAGAACACCT
  GACAGGACCAAAGAGAATGGATCAGACCGGGACCCTCTGCACCCCGACCACCTCAGTAAGCGGTCCTGCA
  CCCTGAGCCCCGCCCAGCGCTGCAGCCCCAGCAATGGGCTGCCCCACCCGACGCCACCCCCACCCCCGCA
  CTATCGCCTGGAGGACATGGCCATGGCCCACCATTTCCGGGACTCCTACCGCCATCCTGATCCCCGAGAG
  CTACGGGAACGCCACCGGCCCCTGGCCATACCTGGGTCTCGACAAGAAGAAGTGATTGATCACAGGCTCA
  CAGAACGCGAGTGGGCAGAAGAATGGAAGCACCTCAACAGTCTTCTGAACTGCATCATGGACATGGTGGA
  GAAGACCCGGCGATCCCTCACCGTCCTGCGCCGGTGTCAGGAGGCCGACCGTGAGGAACTCAACCACTGG
  ATCCGGTGCTACAGTGACTCTGAGGAGGGGAAGAAGGGCCCTACCCCCATCTCTGCCCGGTCCCTCAACA
  GCTGCAGTGGCCCTGAGGGGTCTCAGCTAGATGTTCACCGGGACTTCACGCCCAGGACCCTGTCTGGCTA
  CATGCCTGAAGAGATCTGGAGGAAGGCTGAAGAAGCTGTGAATGAGGTGAAGCGCCAGGCCATGTCAGAA
  CTACAGAAAGCTGTGTCTGATGCGGAGCGCAAAGCCCATGAACTCATCACCACAGAGCGTGCCAAGATGG
  AACGAGCCCTGGCGGAGGCCAAGCGACAGGCCTCGGAGGATGCCCTGACTGTCATCAACCAGCAAGAGGA
  CTCCAGCGAGAGCTGCTGGAACTGCGGGCGCAAGGCCAGCGAGACGTGCAGTGGCTGTAACGCCGCCCGC
  TACTGCGGGTCCTTCTGTCAGCACAAAGACTGGGAGAAACACCATCACGTGTGCGGCCAGAGTCTGCAGG
  GCCCCGCGGCTGCAGTGGCTGACCCACTACCTGGACAGCCTGACGCCACTGCCAGCCCCAGCGAAGCCGG
  CTCGGCAGGGCCCTCTCGTCCCTGCTCTCCGGGGCCGCCAGGCCCGCTGGACGCTGCTGTGCCCCGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177289
• Insert Size: 1680 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177289.1, NP_796263.1
• RefSeq Size: 7600 bp
• RefSeq ORF: 1680 bp
• Locus ID: 12398
• UniProt ID: O54972
• Cytogenetics: 8 E1
• Gene Summary: Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes. Can repress the expression of MMP7 in a ZBTB33-dependent manner. Reduces the protein levels and stability of the transcriptinal regulator HIF1A; interacts with EGLN1 and promotes the HIF1A prolyl hydroxylation-dependent ubiquitination and proteasomal degradation pathway. Contributes to inhibition of glycolysis and stimulation of mitochondrial respiration by down-regulating the expression of glycolytic genes including PFKFB3, PFKFB4, PDK1, PFKP, LDHA and HK1 which are direct targets of HIF1A (By similarity). Regulates the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes (PubMed:16407974). Plays a role in granulocyte differentiation (PubMed:15231665).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR and lacks two in-frame portions of the 5' coding region compared to variant 1. The resulting protein (isoform 3) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.