Rftn1 (NM_181397) Mouse Untagged Clone

CAT#: MC218927

Rftn1 (untagged) - Mouse raftlin lipid raft linker 1 (Rftn1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rftn1
• Synonyms: 2310015N21Rik; AI853526
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218927 representing NM_181397
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTTGCAGTTTGAACAAGCTGGAGAAACGTGAAGAAAAGCGGCCTGGGAACATTTATTCAACTCTGA
  AGAGACCTCAGGTGGAGACCAAAGTAGATGTGACCTATGAGTACTGCTTTCTAGAGTTCACCACACTGAC
  TGCCGCGGAGCTGCCGAGATCCTCCGCCACGAGGTTAGCCTCTCTGCGGGACCTGCCTGACCAGCTTCTG
  GAGCTGTACCAACAAGGCTTCTCACTGGCCGCCCTGCACCCATTTGTGCAGCCAACCCGCAGGCAAGAGA
  AGATCCTCCTGGAACACATCTTTCGGGCCATCCTGGTCAAGAAAACCAACAGGTCTCAGAAGGCTGAGCT
  TCACGACGAAGGCTACACCCTGGAATTGGATTACTGCTCCTCCTTAGAACACCTGGCCGACCAGAAGCTC
  ATCCCAGAGTTCATTAAGAAGGTGCAGGAGGCCGCCAGTCAGGGCTTGAAGTTTGTTAGCGTGGTGCCCC
  AGTACCAGCCCTCGGTGAGCTCAGCAGGCAGCCGGCGTCTCAAGCCTGTTGCCAACAGTGTTGAAGATGC
  CAGGGATGTGAAATGCACCTTAGGGGACCGTTCATCCTTGGAAAATGATACTCCGAAGGCTGCAGAGACG
  GATGCTGCCACTGCAGGAGTGAACCGAAGACCGGAGACCAAACCAGGATCCACAGGGGACGTGCCCAGTG
  CCCAGCAGCCTGGCATTCCCTCTCCCTCGGCAGAGAATGAGGCTGGCGAGTTTCCACTGCGGGGTTTGCA
  GCCAGCACTGGATAGATCAGAGGGCGACCCCTCAAATGGGCCTGAAGAGCTGCCCTCCAGGAAGATGGAG
  ATCTTCGCCTTTTTCAACAGACCGAAGAGCCAGCAGAAATGTCGGCAGTACTACCCTGTCACCATCCCCC
  TCCAGGTCTCCAAGAACGGCCAGACAGTGAGCAGTCTAGACGCCAGCTGGCTGGAACACATGAGTGACCA
  TTTCCGGAAAGGCGGTGTGCTGGTGAACGCCGTCTTCCAGCTTGGCATGGCCAATGATTCCTTCTATGGC
  CTGACAGATGGAGTATTCATCTTTGAAGCTGTTTCCACAGAAGATAACAGAACCACACAGGGCTATGACG
  CTATTGTTGTGGAACAATGGACAGTCCTAGAAGGTACAGAGGTACAGACCGACTACATGCCCCTGCTGAA
  CTCGCTGGCGGCCTACGGATGGCAGCTCACCTGTGTGCTGCCAACGCCTATACTCAAGACTACCAGGGAA
  GGGAACGTGTCCACCAAGCAGATTGTGTTTCTTCAGAGACCATGTCTACCTCAGAAAACCAAGAAGAGGG
  AATCTAAGTTCCAGTGGCGATTCTCCCGGAACGAGATCCACGGCAGGCAGACAAGGAAATCCAAAGGGAA
  ACTCAGTGCCAGCAACAAGCAGCAAGCAGAAGAGAATGAGAAGAATCTGGAAGACCAGTTCTCCAAGGCT
  GGAGATGTGGGAAACTGTGTGCTGGGGGCACCGCAGTGGGGCAGAGCCTCTGAGGTCAGGGAGCAAAGGC
  AGGGCTCAGCAGCTGTGCAGAATGGTCCCGCTGGCCACAACAGGGACTCGGTGGCCCTTAGGCACTCGAA
  TCCCAGAGCTGAGGCTGAGCTTGCTGCAGGCCCCACTCCAACAGAAGCAAATTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_181397
• Insert Size: 1665 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181397.2, NP_852062.1
• RefSeq Size: 2802 bp
• RefSeq ORF: 1665 bp
• Locus ID: 76438
• UniProt ID: Q6A0D4
• Cytogenetics: 17 C
• Gene Summary: Involved in protein trafficking via association with clathrin and AP2 complex (By similarity). Upon bacterial lipopolysaccharide stimulation, mediates internalization of TLR4 to endosomes in dendritic cells and macrophages, and internalization of poly(I:C) to TLR3-positive endosomes in myeloid dendritic cells and epithelial cells; resulting in activation of TICAM1-mediated signaling and subsequent IFNB1 production (PubMed:27022195). Involved in T-cell antigen receptor-mediated signaling by regulating tyrosine kinase LCK localization, T-cell dependent antibody production and cytokine secretion (PubMed:19414744). May regulate B-cell antigen receptor-mediated signaling (By similarity). May play a pivotal role in the formation and/or maintenance of lipid rafts (By similarity).[UniProtKB/Swiss-Prot Function]