Lgi2 (NM_144945) Mouse Untagged Clone

CAT#: MC218898

Lgi2 (untagged) - Mouse leucine-rich repeat LGI family, member 2 (Lgi2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lgi2
• Synonyms: mKIAA1916
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218898 representing NM_144945
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTATGGAGAGGCGGCGGGGCGCTGGGGTTGCTGCTGCTGAGCGCCGCGTGCCTCATCCCGCCGA
  GCGCGCAGGTGAGGCGGTTGGCGCGCTGCCCCGCCACTTGCAGCTGTACCAAGGAGTCCATCATCTGCGT
  GGGCTCCTCTTGGGTGCCTAGGATCGTGCCTGGCGACATCAGCTCCCTGAGCCTAGTAAATGGAACATTT
  CTGGAAATCAAGGACCGAATGTTTTCCCACCTGCCCTCTCTGCAGCTGCTATTGCTGAATTCTAACTCAT
  TCACGGTGATCCGGGACGATGCCTTTGCTGGCCTTTTTCATCTTGAATACCTGTTCATTGAAGGGAACAA
  AATAGAAACCATTTCAAGAAATGCCTTTCGGGGCCTCCGTGACCTGACTCACCTAGACTTAAGGGGTAAT
  AAATTTGAATGTGACTGCAAAGCCAAATGGCTGTATCTATGGCTGAAGATGACAAACTCCACCGTCTCTG
  ATGTGCTGTGTATCGGTCCACCAGAATACCAGGAGAAGAAACTCAACGAAGTGACCAGCTTTGACTATGA
  GTGTACCACCACAGGTCCCCAGACTGATGAAGCCAAGCAGAGAGGATGGCAGTTGGAACTCTCCCTGGGA
  TTTTGTGAACTAATATTTGTTTTTCAACACCCACTCTCAGATTTTGTCGTTCATCAGACTCTGCCGTACC
  AGTCGGTGTCAGTAGACACGTTCAACTCCAAGAACGATGTGTACGTGGCCATCGCTCAGCCCAGCATGGA
  GAACTGCATGGTGTTGGAGTGGGACCACATAGAAATGAATTTCCGGAGTTATGACAATATCACAGGCCAG
  TCCATCGTGGGCTGCAAGGCCATCCTCATTGACGACCAGGTCTTTGTGGTGGTGGCCCAGCTCTTCGGTG
  GCTCTCACATTTACAAATACGACGAGAGCTGGACCAAGTTTGTCAAGTTCCAAGACATAGAGGTGTCTCG
  GATTTCCAAGCCCAACGACATTGAGCTGTTCGAGATCGACGACGAGACCTTCTTCATCATCGCCGACAGC
  TCCAAAGCGGGGCTGTCCACAGTTTACAAGTGGAACAGCAAAGGGTTTTACTCCTACCAGTCACTACATG
  AGTGGTTCAGGGACACAGATGCGGAGTTTGTTGACATCGACGGGAAGTCTCACCTCATCCTGTCTAGCCG
  CTCCCAGGTCCCCATCATCCTGCAGTGGAATAAAAGTTCCAAGAAGTTTGTCCCCCATGGTGACATCCCC
  AATATGGAGGATGTGCTGGCTGTGAAGAGCTTCCGGATGCAGAACACCCTCTACCTTTCCCTCACCCGTT
  TCATTGGGGACTCGCGGGTCATGCGTTGGAACAGTAAGCAGTTTGTGGAGGTCCAGGCTCTTCCTTCCCG
  GGGGGCTATGACCTTGCAACCCTTCTCCTTCAAAGATAACCACTACCTGGCCCTGGGGAGTGACTACACG
  TTCTCACAGATCTACCAGTGGGATAAAGAGAAACAGCAGTTTAAAAAGTTTAAAGAAATCTATGTGCAGG
  CGCCTCGGTCCTTCACAGCCGTCTCCACCGACAGGAGAGATTTCTTTTTTGCATCCAGTTTCAAAGGGAA
  GACAAAGATTTTTGAACATATCATTGTTGATTTAAGTTTGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_144945
• Insert Size: 1653 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144945.3, NP_659194.1
• RefSeq Size: 6315 bp
• RefSeq ORF: 1653 bp
• Locus ID: 246316
• UniProt ID: Q8K4Z0
• Cytogenetics: 5 C1
• Gene Summary: Required for the development of soma-targeting inhibitory GABAergic synapses made by parvalbumin-positive basket cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encode the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.