Slc8b1 (NM_001177595) Mouse Untagged Clone

CAT#: MC218871

Slc24a6 (untagged) - Mouse solute carrier family 24 (sodium/potassium/calcium exchanger), member 6 (Slc24a6), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Slc8b1
• Synonyms: AF261233; NCKX6; NCLX; Slc24a6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218871 representing NM_001177595
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAGCAGATGGCTGGCTCTGCTCTGGGCTCCTGTCTTCCTCTGTGTGGCTCTGATACTGGAGACGG
  CGTCTGGGACCGGAGACCCATCCACAAAAGCCCATGGACACATCCAGTTTTCAGCTGGAAGTGTCAACCA
  GACTGCCATGGCGGATTGCCGAGCCGTGTGTGGCCTGAACACATCTGATCGCTGTGACTTTGTCAGGAGG
  AATCCGGACTGCCGCAGCGAGGCGGGCTACCTGGACTACCTTGAGGGCATCTTCTGCTACTTCCCCCCCA
  ACCTCCTCCCTCTGGCCATCACCCTCTACGTTTTCTGGCTGCTTTACCTCTTTCTGATCCTGGGAGTCAC
  CGCGGCCAAGTTCTTCTGCCCTAACCTGTCAGCCATCTCCACTAACCTCAAACTCTCCCACAACGTGGCA
  GGTGTCACCTTCCTGGCCTTTGGAAATGGCGCTCCAGACATCTTCAGTGCTTTAGTGGCTTTCTCAGACC
  CACGTACTGCCGGCCTGGCCATCGGGGCTCTGTTTGGTGCAGGGGTGCTGGTCACCACTGTGGTGGCTGG
  AGGCATCACCATCCTGCATCCCTTCATGGCTGCCTCCAGGCCCTTCCTCAGGGACATCGCTTTCTACATG
  GTGGCTGTGTTCCTAACCTTCACTGCACTCTATCTCGGCAGGATCACGCTGACGTGGGCGCTGGGTTACC
  TGGGCCTCTACGTGTTCTACGTGGTCACGGTCATCATCTGCACTTGGGTCTACCAACGGCAGCGAAGCAG
  GTCTCTGGTCCACTCCATATCGGAGACACCAGAGTTGCTGTCTGAGTCAGAGGAGGACCAGATGTCTTCC
  AACACCAACAGCTATGACTATGGAGATGAATACCGGCCTCTGTTGCTGGGTCGGGAGACCACTGTCCAGA
  TCCTGATCCAAGCCCTGAATCCCTTGGACTACAGGAAGTGGAGAACTCAGTCGATATCCTGGAGGGTCCT
  GAAGGTAGTCAAGTTGCCTGTGGAGTTCTTGTTGCTGCTCACAGTACCGGTTGTGGACCCCGACAAGGAC
  GATCGGAATTGGAAACGGCCACTCAACTGTCTGCAGCTGGTCATCAGCCCCCTGGTCCTGGTCCTGACCC
  TGCAGTCGGGGGTCTATGGCATCTATGAGATCGGCGGTCTCCTTCCTGTCTGGGCTGTGGTGGTGATCGT
  GGGCACAGCGCTGGCTTCAGTGACCTTCTTTGCCACGTCTAACAGAGAACCCCCTAGACTGCACTGGCTC
  TTTGCTTTCCTGGGTTTCCTGACCAGTGCCCTGTGGATCAATGCAGCCGCCACAGAGGTGGTGAACATAT
  TACGGTCCCTGGGTGTGATCTTCCGCCTGAGCAACACCGTCCTAGGGCTGACCCTCCTGGCCTGGGGAAA
  CAGCATTGGAGCTGGAGCCAGACGGATTACTGGTGTGGGTGCTGGCCAGTGCCCTGGGCCTCAGCTTGAT
  CTTCTCCCTGGTCTCCGTGCCGCTTCAGTGTTTCCAGCTCAGCAAGGCTTACGGCCTCTGCCTCCTCCTC
  TTCTACATCTGTTTCCTTGTTGTGGTCCTGCTCACAGAGTTTGGGGTGATTCACCTGAAGAAGGCGTGAC
  TGAAGCTGCTTGGCCTAGAGGTGTGGGGGCGATTCTGCTAGCCTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001177595
• Insert Size: 1659 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001177595.1, NP_001171066.1
• RefSeq Size: 2725 bp
• RefSeq ORF: 1659 bp
• Locus ID: 170756
• UniProt ID: Q925Q3
• Cytogenetics: 5 F
• Gene Summary: Mitochondrial sodium/calcium antiporter that mediates sodium-dependent calcium efflux from mitochondrion, by mediating the exchange of 3 sodium ions per 1 calcium ion (PubMed:20018762, PubMed:28445457). Plays a central role in mitochondrial calcium homeostasis by mediating mitochondrial calcium extrusion: calcium efflux is essential for mitochondrial function and cell survival, notably in cardiomyocytes (PubMed:24067497, PubMed:28445457). Regulates rates of glucose-dependent insulin secretion in pancreatic beta-cells during the first phase of insulin secretion: acts by mediating efflux of calcium from mitochondrion, thereby affecting cytoplasmic calcium responses (By similarity). Required for store-operated Ca(2+) entry (SOCE) and Ca(2+) release-activated Ca(2+) (CRAC) channel regulation: sodium transport by SLC8B1 leads to promote calcium-shuttling that modulates mitochondrial redox status, thereby regulating SOCE activity (By similarity). Involved in B-lymphocyte chemotaxis (PubMed:27328625). Able to transport Ca(2+) in exchange of either Li(+) or Na(+), explaining how Li(+) catalyzes Ca(2+) exchange (By similarity). In contrast to other members of the family its function is independent of K(+) (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1. The resulting isoform (3) lacks an internal segment near the C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.