Trim9 (NM_001110203) Mouse Untagged Clone

CAT#: MC218861

Trim9 (untagged) - Mouse tripartite motif-containing 9 (Trim9), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim9
• Synonyms: AI835002; C030048G07Rik; mKIAA0282
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218861 representing NM_001110203
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGAGATGGAAGAAGAGTTAAAATGCCCTGTGTGTGGCTCCTTCTATCGGGAGCCCATCATCTTGC
  CCTGCTCTCACAATTTATGTCAGGCGTGCGCCCGCAACATCCTGGTGCAGACCCCGGAGTCCGAGTCCCC
  CCAGAGCCGCCGGGCCTCGGGCTCTGGGGTTTCTGACTATGATTATCTGGACCTGGACAAGATGAGCCTG
  TACAGCGAGGCGGACAGCGGCTATGGCTCCTACGGAGGTTTCGCCAGCGCCCCCACTACCCCGTGCCAGA
  AGTCGCCCAACGGCGTCCGCGTTTTCCCCCCTGCTATGCCGCCACCGCCCACCCACCTGTCACCGGCTTT
  GGCCCCTGTGCCCCGCAATTCCTGCATCACCTGCCCCCAGTGCCACCGCAGCCTCATTCTGGATGACCGG
  GGGCTCCGCGGTTTCCCCAAGAACCGCGTCCTGGAAGGGGTCATCGACCGCTACCAACAGAGCAAAGCCG
  CGGCCCTCAAGTGCCAGCTCTGCGAGAAGGCGCCCAAGGAAGCCACGGTCATGTGTGAACAGTGCGATGT
  CTTCTACTGCGACCCTTGCCGTCTGCGCTGCCACCCGCCCCGGGGTCCCCTAGCCAAACACCGTCTGGTG
  CCCCCGGCCCAGGGCCGCGTCAGCCGGCGGCTGAGCCCGCGCAAGGTCTCCACCTGCACAGACCACGAGC
  TGGAGAACCACAGCATGTACTGCGTGCAATGCAAGATGCCCGTGTGCTACCAGTGCCTAGAGGAGGGCAA
  ACACTCCAGCCACGAAGTCAAGGCTTTGGGGGCTATGTGGAAATTGCACAAGAGCCAGCTCTCCCAGGCC
  CTGAATGGATTGTCGGACAGGGCCAAAGAAGCCAAGGAGTTCCTGGTGCAGCTCCGCACCATGGTACAAC
  AGATCCAGGAAAACAGTGTGGAGTTTGAGGCCTGCCTGGTGGCCCAGTGTGATGCGCTCATCGATGCCCT
  AAACCGAAGGAAGGCTCAGCTGCTGGCCCGGGTCAACAAGGAGCATGAACACAAGCTGAAGGTGGTTCGG
  GACCAGATCTCTCACTGCACGGTGAAGCTGCGTCAGACCACGGGCCTCATGGAGTACTGCTTGGAAGTGA
  TTAAGGAGAACGACCCCAGTGGCTTTTTGCAGATTTCAGATGCTCTCATAAGGCGAGTTCACTTAACTGA
  GGACCAGTGGGGGAAGGGCACCCTCACTCCCAGGATGACCACAGACTTTGATCTGAGCCTGGACAACAGC
  CCTCTGCTGCAGTCTATTCACCAACTGGACTTCGTGCAAGTGAAAGCCTCCTCTCCAGTCCCAGCAACCC
  CCATCCTACAGCTGGAGGAGTGTTGCACCCACAACAACAGTGCTACGCTGTCCTGGAAACAGCCCCCTCT
  GTCCACCGTAGCCGCCGATGGATACATCCTGGAGCTGGATGACGGCAGTGGTGGTCAGTTCCGGGAAGTG
  TATGTTGGAAAAGAAACAATGTGCACAGTGGACGGCCTTCACTTCAACAGCACATACAACGCTCGGGTTA
  AGGCCTTCAACAAAACAGGAGTCAGCCCCTACAGCAAGACACTGGTCCTCCAGACGTCTGAGGCAGCGGG
  AGCCCATGAGACAAAACCTATGAAGGGAAGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001110203
• Insert Size: 1644 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110203.1, NP_001103673.1
• RefSeq Size: 4843 bp
• RefSeq ORF: 1644 bp
• Locus ID: 94090
• UniProt ID: Q8C7M3
• Cytogenetics: 12 C2
• Gene Summary: E3 ubiquitin-protein ligase which ubiquitinates itself in cooperation with an E2 enzyme UBE2D2/UBC4 and serves as a targeting signal for proteasomal degradation. May play a role in regulation of neuronal functions. May act as a regulator of synaptic vesicle exocytosis by controlling the availability of SNAP25 for the SNARE complex formation.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks several exons, and contains an alternate 3'-terminal exon, compared to variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (c) has a shorter and distinct C-terminus, compared to isoform a.