Dbr1 (NM_031403) Mouse Untagged Clone

CAT#: MC218858

Dbr1 (untagged) - Mouse debranching enzyme homolog 1 (S. cerevisiae) (Dbr1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dbr1
• Synonyms: AW018415
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218858 representing NM_031403
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGTGGCCGTGGCCGGCTGCTGCCACGGCGAGCTGGACAAGATCTACGAGACTCTGGCGCTGGCGG
  AGCGGCGCGGCTCGGGACCCGTGGATCTCCTTCTGTGCTGCGGCGACTTCCAGGCGGTGCGCAACGAGGC
  CGACCTGCGCTGCATGGCCGTGCCGCCCAAGTACCGTCACATGCAGACCTTCTACAGATATTACTCTGGA
  GAGAAAAAGGCCCCGGTTCTCACCATCTTCATTGGAGGAAACCATGAAGCCTCAAACCATTTGCAAGAGT
  TACCCTATGGTGGCTGGGTAGCGCCGAATATTTATTATTTAGGCTTGGCTGGTGTTGTCAAATACCGAGG
  AGTAAGGATTGGTGGCATCTCTGGCATATTTAAATCTCATGATTATAGAAAAGGTCATTTTGAGTGCCCC
  CCTTATAATTCATCTACCATAAGGAGTATATATCATGTGAGAAATATAGAGGTCTACAAATTAAAACAGT
  TAAAGCAGCCTGTACATATATTCTTGTCTCATGATTGGCCAAGAAATATATATCATTATGGCAATAAGAA
  GCAGCTTCTTAAGACCAAATCTTTTTTCCGCCAAGAAGTGGAAAACAGCACCCTGGGGAGCCCCGCTGCC
  TCGGAGCTGCTGGAGCACCTACAGCCTGCGTACTGGTTCTCCGCGCACCTGCATGTCAAGTTCGCAGCCC
  TGATGCAGCATCAGGCCACAGATAAAGACCAAGCAGGCAAAGAAACCAAGTTTTTAGCCTTGGACAAGTG
  CTTGCCACACAGAGACTTCCTTCAGGTGTTAGAGATAGAACATGACCCCAGTGCTCCTGAGTACTTAGAG
  TATGACGTTGAATGGCTTACTGTTCTCAGGGCCACTGATGACCTGATTAATGTGACTGGGGGCCTGTGGA
  ATATGCCTGAAGACAACGGTCTGCACACAAGGTGGGATTATAGTGCAACGGAAGAAACTATGAAGGAAGT
  AATGGAAAAATTGAACCATGACCCTAAAGTCCCCTGTAACTTCACCATGACAGCTGCTTGTTATGATCCC
  AGTAAGCCCCAGACACAAGTGAAGCTGGTTCACAGGATCAATCCACAGACGACTGAGTTCTGTGCCCAGC
  TTGGCATCACAGACATCAATGTCATGATTCAGAAGGCCAGAGAGGAAGAGCACCATCAGTGTGGTGAATA
  TGAGCAGCAGGGTGACCCGGGGACTGAGGAGTCTGAAGAGGACCGGAGTGAGTACAACACAGACACGTCG
  GCCCTCTCCTCCATTAATCCAGATGAAATCATGCTGGATGAAGAGGAGGAGGAGGAGGAGGAGGAGGAGG
  AGGCTGTAAGCGCTCACAGTGACATGAACACACCTTCTGTGGAACCTGCTTCTGACCAGGCTTCTGACCT
  CTCTACAAGCTTTTCTGACATCAGGAACTTACCAAGTTCCATGTTTGTGTCCTCTGACGATGCATCCCGT
  TCTCCAGCAAGTGGAGAGGGGAAATGTGGTGAAACGGTGGAGTCTGGGGATGAAAAAGACTTGGCTAAGT
  TTCCTCTGAAGCGGCTGAGTGATGAACACGAACCTGAACAAAGGAAGAAAATAAAGAGGAGGAACCAAGC
  CATCTACGCTGCAGTGGATGATGGGGATGCTAGTGCCGAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_031403
• Insert Size: 1653 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031403.3, NP_113580.2
• RefSeq Size: 2103 bp
• RefSeq ORF: 1653 bp
• Locus ID: 83703
• UniProt ID: Q923B1
• Cytogenetics: 9 E3.3
• Gene Summary: Cleaves the 2'-5' phosphodiester linkage at the branch point of lariat intron pre-mRNAs after splicing and converts them into linear molecules that are subsequently degraded. It thereby facilitates ribonucleotide turnover. It may also participate in retrovirus replication via an RNA lariat intermediate in cDNA synthesis (By similarity).[UniProtKB/Swiss-Prot Function]