Zmynd11 (NM_144516) Mouse Untagged Clone

CAT#: MC218834

Zmynd11 (untagged) - Mouse zinc finger, MYND domain containing 11 (Zmynd11), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zmynd11
• Synonyms: 2210402G22Rik; BS69
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218834 representing NM_144516
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACGTTTAACAAAAAGACGACAGGCGGATACAAAAGCTATCCAGCATCTTTGGGCAGCCATTGAAA
  TTATCAGAAATCAGAAGCAGATTGCCAACATTGATCGTATTACAAAGTATATGTCCCGAGTCCACGGTAT
  GCATCCCAAAGAGACCACCCGCCAGCTGAGCTTAGCTGTGAAAGATGGTCTTATTGTCGAAACTCTAACA
  GTGGGCTGCAAAGGTTCAAAAGCTGGTATTGAACAGGAAGGATATTGGTTACCAGGAGATGAGATTAGCA
  TTAAGAAGAAGCACTCTAACAAGCAGGAAATGGGCACCTACTTGAGGTTCATCGTCTCCCGAATGAAGGA
  GCGGGCTATAGACCTTAATAAGAAAGGAAAGGACAGTAAGCATCCTATGTACCGGAGGCTGGTCCACTCA
  GCTGTTGACGTCCCTACCATACAAGAGAAGGTGAATGAGGGGAAGTACCGGAGTTACGAGGAGTTCAAGG
  CTGACGCACAGCTGCTTCTCCACAACACAGTGATCTTCTATGGAGCAGACAGTGAGCAGGCGGACATTGC
  ACGGATGTTATATAAAGACACGTGTCACGAGCTGGACGAGCTGCAGCTTTGCAAGAACTGCTTCTATCTG
  TCAAACGCACGGCCCGACAACTGGTTCTGCTACCCTTGCATACCTAATCATGAGCTGGTTTGGGCTAAAA
  TGAAAGGTTTTGGGTTTTGGCCAGCCAAAGTCATGCAGAAAGAAGACAATCAAGTGGATGTTCGCTTCTT
  TGGCCATCACCACCAGAGAGCCTGGATTCCTTCTGAAAACATCCAGGATATCACGGTCAATGTTCACCGA
  CTGCACGTGAAGCGCAGCATGGGCTGGAAGAAGGCCTGTGACGAGCTGGAACTGCATCAGCGCTTCCTCC
  GTGAAGGAAGGTTCTGGAAGTCTAAGAATGAGGACCGAGGTGAGGAAGAGGCAGAATCCAGTATCTCCTC
  CACCAGTAACGAGCAGCTGAAGGTCACTCAAGAGCCAAGAGCAAAGAAAGGACGACGTAATCAGAGTGTG
  GAGCCCAAAAAGGAAGAACCCGAGCCAGAAACGGAAGCAGTGAGTTCCAGCCAGGAAATCCCCACAATGC
  CTCAGCCAATTGAGAGGGTGTCAGTGTCAACCCAGACCAAGAAGTTAAGTGCCTCTTCCCCACGAATGCT
  GCATCGAAGCACTCAGACGACGAGCGACGGCGTCTGTCAGAGCATGTGCCACGACAAGTACACCAAAATT
  TTTAATGACTTTAAAGACAGGATGAAGTCGGACCACAAGCGGGAAACAGAGAGAGTGGTCCGTGAAGCGC
  TGGAAAAGTTGCGTTCTGAAATGGAAGAAGAAAAAAGACAGGCCGTGAACAAAGCTGTGGCCAGCCTGCA
  GGGCGACATGGACAGAAAGGGTAAGCAGCTGAAGGAGAAGTGCAAGGAAGAGTTTGTAGAGGAGATCAAG
  AAGCTAGCGGCGCAGCACAAGCAGCTCATTTCCCAGACCAAGAAGAAGCAGTGGTGCTACAACTGTGAGG
  AGGAGGCCATGTACCACTGCTGCTGGAACACGTCCTACTGCTCCATCAAGTGCCAGCAGGAGCACTGGCA
  CGCAGAGCACAAGCGCACCTGCCGCCGGAAGAGATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_144516
• Insert Size: 1647 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144516.2, NP_653099.2
• RefSeq Size: 3970 bp
• RefSeq ORF: 1647 bp
• Locus ID: 66505
• Cytogenetics: 13 A1
• Gene Summary: Chromatin reader that specifically recognizes and binds histone H3.3 trimethylated at 'Lys-36' (H3.3K36me3) and regulates RNA polymerase II elongation. Does not bind other histone H3 subtypes (H3.1 or H3.2). Colocalizes with highly expressed genes and functions as a transcription corepressor by modulating RNA polymerase II at the elongation stage (PubMed:24590075). Binds non-specifically to dsDNA (By similarity). Acts as a tumor-suppressor by repressing a transcriptional program essential for tumor cell growth (PubMed:24590075).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) differs in the 5' UTR, lacks an alternate in-frame exon in the 5' coding region and uses an alternate in-frame splice site in the 3' coding region, compared to variant 8. The encoded isoform (alpha) is shorter than isoform beta. Variants 1, 6 and 7 all encode isoform alpha.