Nectin3 (NM_021495) Mouse Untagged Clone

CAT#: MC218833

Pvrl3 (untagged) - Mouse poliovirus receptor-related 3 (Pvrl3), transcript variant alpha, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nectin3
• Synonyms: 2610301B19Rik; 3000002N23Rik; 4921513D19Rik; AA407785; AU016832; AW538082; CD113; Pvrl3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218833 representing NM_021495
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCGGACCCCGGGCCCGGCCCCGTTGTGTCCTGGAGGCGGCAAAGCACAACTTTCCTCGGCGTTTC
  CTCCCGCGGCCGGACTGCTGCTGCCGGCCCCGACGCCGCCGCCGCTGCTGCTGCTGCTTATTCCCCTGCT
  TCTCTTCTCCCGGCTCTGTGGTGCCTTAGCTGGATCAATTATTGTGGAGCCACATGTCACAGCAGTGTGG
  GGAAAGAATGTTTCATTGAAGTGTTTAATTGAAGTGAATGAAACTATAACCCAGATCTCATGGGAGAAGA
  TACATGGCAAAAGTACACAGACTGTTGCAGTTCATCATCCTCAGTATGGATTCTCTGTTCAAGGAGATTA
  TCAGGGAAGAGTCTTGTTTAAAAACTATTCACTTAATGATGCAACAATTACTCTGCATAACATAGGCTTC
  TCAGATTCTGGAAAATATATATGCAAAGCCGTTACATTCCCACTTGGAAATGCTCAGTCCTCTACAACAG
  TGACTGTGTTAGTTGAACCCACAGTGAGCCTGATAAAAGGGCCGGATTCTTTAATTGATGGAGGGAATGA
  GACAGTAGCAGCCGTTTGTGTAGCAGCCACTGGAAAGCCAGTCGCACAGATTGACTGGGAAGGTGATCTT
  GGTGAAATGGAATCTAGTACAACTTCTTTTCCTAATGAAACAGCAACGATTGTCAGCCAATACAAGCTGT
  TTCCCACAAGATTTGCTCGAGGAAGGCGAATTACTTGTGTTGTAAAACATCCAGCCTTAGAAAAGGACAT
  TCGCTACTCTTTCATACTAGACATACAGTATGCTCCTGAAGTTTCAGTAACAGGATATGATGGAAATTGG
  TTCGTGGGAAGAAAAGGTGTTAACCTCAAGTGTAATGCTGATGCAAACCCTCCACCCTTCAAGTCCGTGT
  GGAGCAGGTTGGATGGACAATGGCCTGATGGTTTATTGGCGTCAGATAATACTCTTCATTTTGTCCATCC
  ATTGACTGTCAATTATTCTGGCGTTTATGTCTGTAAAGTATCAAATTCCCTTGGTCAAAGAAGTGATCAA
  AAGGTTATCTACATTTCAGATCCTCCTACCACCACCACCCTTCAGCCGACAGTTCAGTGGCATTCCTCAC
  CTGCTGACGTCCAGGATATAGCAACAGAGCATAAAAAATTGCCCTTTCCTTTGTCAACTTTGGCAACACT
  TAAGGATGACACAATTGGCACCATCATTGCTAGTGTAGTGGGTGGGGCTCTCTTCTTAGTGCTTGTGAGC
  ATTTTAGCTGGGGTATTCTGCTATAGGAGACGACGGACGTTTCGTGGAGACTACTTTGCCAAAAACTACA
  TTCCACCATCAGACATGCAGAAAGAATCACAGATTGATGTTCTTCACCAGGATGAGCTGGATTCTTACCC
  AGACAGTGTAAAAAAGGAAAACAAAAATCCAGTAAACAACCTGATCCGCAAAGACTACTTAGAGGAGCCT
  GAGAAAACTCAGTGGAATAATGTAGAGAACCTCACTAGGTTTGAAAGACCGATGGATTACTATGAAGATC
  TAAAAATGGGAATGAAGTTTGTCAGTGATGAACGCTACAATGAAAGTGAAGATGGTTTGGTTTCTCATGT
  AGATGGCTCCGTAATTTCCAGGAGGGAGTGGTATGTCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_021495
• Insert Size: 1650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021495.4, NP_067470.1
• RefSeq Size: 3085 bp
• RefSeq ORF: 1650 bp
• Locus ID: 58998
• UniProt ID: Q9JLB9
• Cytogenetics: 16 B5
• Gene Summary: Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectins-like or other nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (alpha) represents the longest transcript and encodes the longest isoform (alpha). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.