Pde1a (NM_016744) Mouse Untagged Clone

CAT#: MC218814

Pde1a (untagged) - Mouse phosphodiesterase 1A, calmodulin-dependent (Pde1a), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pde1a
• Synonyms: AI987702; AW125737
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218814 representing NM_016744
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTCTACTGATACGGACATCGAAGAATTGGAAAACGCCACTTACAAGTATCTCATTGGAGAACAGA
  CCGAGAAGATGTGGCAGCGCCTGAAGGGAATACTAAGATGCTTAGTGAAGCAGCTGGAGAAAGGTGATGT
  CAACGTTGTTGACCTAAAGAAGAATATCGAATATGCAGCATCAGTACTGGAAGCTGTTTACATCGATGAG
  ACAAGAAGACTGCTGGACACAGAGGATGAACTCAGCGACATCCAGACTGACTCCGTCCCATCGGAAGTCC
  GGGATTGGTTGGCTTCAACTTTCACACGCAAAATGGGGATGATGAAAAAGAAGCCTGAAGAAAAACCAAA
  GTTTCGCAGCATTGTCCATGCTGTCCAGGCTGGGATTTTTGTGGAAAGGATGTACAGGAAGAACTATCAC
  ATGGTTGGTTTGACATATCCTGCAGCTGTCATAGTGACACTCAAGGAAGTTGATAAATGGTCTTTTGATG
  TATTTGCCTTAAATGAAGCAAGCGGGGAGCATAGCCTGAAGTTTATGATTTATGAACTCTTTACCAGATA
  TGATCTTATCAACCGCTTCAAGATTCCTGTTTCTTGCCTAATTGCCTTTGCAGAAGCCTTAGAAGTTGGT
  TACAGCAAGCACAAAAATCCATATCATAATTTGGTCCATGCAGCTGACGTCACTCAAACTGTGCATTATA
  TAATGCTTCATACAGGTATCATGCACTGGCTCACTGAACTGGAAATTTTAGCCATGGTCTTTGCAGCTGC
  CATTCATGATTATGAGCACACAGGAACAACAAACAACTTCCATATTCAGACGAGGTCGGACGTTGCTATT
  CTGTACAACGACCGCTCAGTGCTTGAGAATCACCACGTCAGCGCAGCCTACAGACTTATGCAAGAGGAGG
  AAATGAATATTTTGGTAAATTTGTCTAAAGATGACTGGAGGGATCTTCGGAACTTAGTGATTGAAATGGT
  CTTAGCGACAGACATGTCAGGGCATTTTCAGCAAATTAAAAACATAAGAAACAGCTTGCAGCAGCCGGAA
  GGGATTGACAGAGCCAAAACTATGTCCCTGATACTCCATGCAGCTGACATCAGCCACCCAGCCAAAACTT
  GGAAGTTGCACTACAGATGGACCATGGCCCTAATGGAGGAGTTTTTCCTGCAGGGAGACAAAGAAGCTGA
  ATTAGGGCTTCCATTTTCTCCACTTTGTGATCGGAAGTCAACAATGGTTGCCCAGTCGCAAATAGGTTTC
  ATTGATTTCATAGTGGAGCCAACGTTTTCTCTCCTGACGGACTCAACAGAGAAAATTGTCATTCCTCTTA
  TAGAGGAAGCTTCAAAGTCACAGTCTTCAAACTACGGGGCAAGCAGCTCGTCCACCATGATTGGGTTCCA
  TGTTGCTGACTCTCTGAGGCGGTCAAATACAAAAGGCTCCGTGTGTGATGGGAGCTATGCCCCAGACTAC
  TCGCTCTCAGCTGTGGATCTGAAGAGTTTCAAAAACAACCTGGTGGACATCATTCAGCAGAACAAAGAGA
  GGTGGAAAGAGTTGGCTGCCCAAGGTGAACTTGATCTTCATAAGAACTCAGAAGAGTTAGGAAATACTGA
  AGAAAAGCATGCTGACACACGTCCATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_016744
• Insert Size: 1638 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016744.4, NP_058024.2
• RefSeq Size: 4309 bp
• RefSeq ORF: 1638 bp
• Locus ID: 18573
• UniProt ID: Q61481
• Cytogenetics: 2 C3
• Gene Summary: Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a higher affinity for cGMP than for cAMP (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, 10, and 11 all encode isoform 2.