Mak (NM_001145802) Mouse Untagged Clone

CAT#: MC218810

Mak (untagged) - Mouse male germ cell-associated kinase (Mak), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mak
• Synonyms: A930010O05Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218810 representing NM_001145802
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCGATACACAACCATGAAGCAGCTGGGAGACGGCACGTATGGGAGTGTACTGATGGGCAAGAGCA
  ATGAGTCTGGGGAGCTGGTGGCAATCAAGAGGATGAAGAGAAAGTTCTATTCTTGGGATGAGTGTATGAA
  CTTGCGAGAAGTTAAGTCCCTGAAAAAACTCAATCACGCCAATGTGATTAAACTAAAAGAAGTTATCAGA
  GAGAATGACCATCTTTATTTTGTATTTGAATATATGAAAGAAAACCTCTATCAGCTAATGAAAGACAGGG
  ACATGAAACCTGAGAACTTGCTCTGCATGGGCCCAGAGCTGGTGAAGATTGCTGATTTTGGACTTGCAAG
  AGAATTAAGATCACAGCCACCATATACTGACTATGTGTCTACCAGATGGTACCGGGCTCCTGAAGTCTTA
  CTAAGGTCTTCGGTGTACAGCTCTCCCATTGACGTGTGGGCCGTGGGAAGTATAATGGCCGAGTTATATA
  CATTTAGGCCGCTTTTCCCAGGGACAAGTGAAGTTGATGAGATCTTTAAAATCTGCCAAGTGTTAGGGAC
  TCCCAAGAAGAGTGACTGGCCGGAAGGATACCAGCTTGCATCCTCCATGAACTTCCGCTTTCCCCAGTGC
  ATTCCCATCAACCTAAAAACTCTCATTCCCAATGCCAGTAGCGAGGCCATCCAGCTCATGACAGAGATGC
  TGAACTGGGATCCAAAGAAGCGACCAACAGCAAGCCAGGCACTGAAACACCCATATTTCCAAGTCGGTCA
  GGTACTGGGCTCTTCTGCACACCATCTGGATACAAAACAGACTTTACACAAGCAGCTGCAGCCTCTGGAG
  CCAAAGCCATCTTCCTCTGAACGGGATCCTAAGCCTTTGCCAAACATACTTGATCAGCCTGCCGGGCAGC
  CCCAGCCAAAACAGGGCCACCAACCACTGCAGACCATTCAGCCACCACAGAACACAGTGACTCACCCACC
  CCCAAAGCAGCAGGGTCACCAGAAACCACCACAAACCATGTTTCCAAGTATCATCAAAACCATCCCAGTG
  AATTCAGTCAGCACCCTGGGCCATAAGGGAGCCCGGAGACGGTGGGGTCAGACAGTCTTCAAGTCTGGAG
  ACAGCTGTGACGACATTGAGGACGACTTAGGAGCCTCCCACTCCAAGAAGCCAAGCATGGAAGCCTGCAA
  GGAGAAGAAGAAGGAGTCTCCATTTCGGTTTCCAGATTCAGGACTCCCAGTCTCCAATCACTTTAAGGGG
  GAAAATAGGAATTTACATGCATCTGTTTCCTTAAAATCTGATCCAAACTTGTCAACTGCTTCAACGGCTA
  AGCAGTACTATTTGAAACAATCAAGATACCTTCCAGGTGTGAACCCCAAGAATGTGTCTTTGGTAGCTGG
  AGGCAAGGATATAAATTCACACTCTTGGAATAATCAGCTATTTCCTAAGTCGCTGGGATCCATGGGGGCG
  GACCTTTCCTTCAAGAGGAGTAACGCAGACTATACCTGGAGCACAAAAACTGGACGAGGCCAGTTTTCGG
  GACGAACTTACAACCCCACAGCCAAAAATCTCAATATTGTGAACCGCACACAGCCAGTCCCCTCGGTACA
  CGGGAGGACAGACTGGGTGGCTAAGTATGGAGGCCACCGGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001145802
• Insert Size: 1653 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145802.1, NP_001139274.1
• RefSeq Size: 3274 bp
• RefSeq ORF: 1653 bp
• Locus ID: 17152
• UniProt ID: Q04859
• Cytogenetics: 13 20.25 cM
• Gene Summary: Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors. Could have an important function in sensory cells and in spermatogenesis. May participate in signaling pathways used in visual and olfactory sensory transduction. Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an alternate segment and uses a different splice site, compared to variant 1. The resulting protein (isoform 3) is shorter when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.