Eif2d (NM_010709) Mouse Untagged Clone

CAT#: MC218809

Eif2d (untagged) - Mouse eukaryotic translation initiation factor 2D (Eif2d), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eif2d
• Synonyms: D1Ertd5e; Lgtn
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218809 representing NM_010709
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCGCTAAGGCCTTTCGGGTCAAGTCCAACACAGCCATCAAGGGCTCGGACAGGAGAAAGCTCCGGG
  CCGATGTGACAGCTGCTTTCCCTGCCCTGGGAACAGATCAGATCTCTGAATTGATACCTGGAAAGGAAGA
  GCTAAATGTTGTGAAGTTGTATGTTCACAAAGGGGATTCGGTGACTGTGTACACGAGTGGTGGTAACCCC
  ATCCTATTTGAACTGGAGAAAAATTTGTATCCAACAGTGTACACACTGTGGGCCTATCCGGATATTCTAC
  CAACTTTTATAACATGGCCTCTGGTATTGGAAAAACTGGTCGGGGGAGCAGATTTGATGCTGCCAGGGGT
  AGTGGTGCCCCCTACTGGTCTTCCTCAAGTACAGCAAGGCGACCTCTGTGCCATTGCCTTGGTGGGGAAC
  AGAGCGCCCGTTGCAATTGGAGTGGCCGCCATGTCCACAGCTCAGATGCTGGCTTCAGGCCTGAAGGGAA
  AAGGTGTCTCTGTGCTCCACACTTATCAGGACCACTTGTGGAGATCTGGAGACAAGTCCTCTCCACCTGC
  CATTGCCCCACTGGACCCCACAGATAGCTGCGAAGAGAAGGTGCACCTTGGTCTGCAGGGAAACCTGAAG
  AGCCTGACCCTGGACGGAGAGGAGGAGAACGGGCAGGTTCCTCTCCGAGAAGCCTCTGAGGACACCAGCT
  CCAGGGCCCCGAGCCAGGATTCCTTGGACGGCAAGCCCCTTCAGGAACAAATGGACGATCTGTTACTGCG
  GTGCTTCCTTCATGCCCTGAAGAGTCGAGTCAAGAAAGCTGACCTCCCGTTGCTCACCAGCACTCTCCTT
  GGCAGTCACATGTTCTCCTGCTGTCCTGAAGGACAGCAACTGGATATAAAGAAGTCAAGCTATAAAAAGC
  TCTCTAAGTTCCTGCAGCACATGCAGCAGGAGCAGATTGTCCAGGTCAAGGAGCTGAGCAAAGGGGTGGA
  GAGCATTGTGGCTGTGGACTGGAGGCACCCGAGGATCACGTCTTTCGTCATCCCCGAGCCTTCCCTGACT
  TCCCAGACTGTCCAGGAAGTTAGCAGGGAACAGCCCTATCTCCCTCCAGATATAAAATCCCTCTACTGTG
  TCCCAGCCAACATGACCCAGCTCTTCCTGGAGTCTGGCCACAAGAAGGGGAGCACCCTTGAGGGCAGTGA
  GGTCCGAAAGATCATCACGGACTATGCCAAGAGAAACAGACTGGTGGATGCAGACAACAGAAATCTGGTG
  AAGTTGGATCCTATCTTATGTGACTGCATCTTAGAGAAAAATGAGCAGCACTTGGTCACGAAGCTCCCAT
  GGGACTGTCTCCTGACCAGGTGTTTGAAAAATATGCAGCCTGCCTACCAAGTAACATTTCCTGGCCAGGA
  ACCCATTCTAAAGAAAGGGAAACTCTGTCCAATTGATATCACCCTTGCACTGAAGACCTATAATAAAAAG
  GTGACTGTGGTCCGGAACTTGGAGACCTATGGTCTAGATCCGTGCTCAGTGGCCGCCATTCTCCAGCAGC
  GATGTCAGGCAAGCACCATCGTCTCTCCTGCCCCTGGGGCCAAAGACAGTCTGCAGGTGCAGGTCCAGGG
  AAACCAGATCCACCACCTTGGCCAGCTACTGCTTGAAATCATGTGGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010709
• Insert Size: 1659 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010709.3, NP_034839.2
• RefSeq Size: 2122 bp
• RefSeq ORF: 1659 bp
• Locus ID: 16865
• UniProt ID: Q61211
• Cytogenetics: 1 56.9 cM
• Gene Summary: Translation initiation factor that is able to deliver tRNA to the P-site of the eukaryotic ribosome in a GTP-independent manner. The binding of Met-tRNA(I) occurs after the AUG codon finds its position in the P-site of 40S ribosomes, the situation that takes place during initiation complex formation on some specific RNAs. Its activity in tRNA binding with 40S subunits does not require the presence of the aminoacyl moiety. Possesses the unique ability to deliver non-Met (elongator) tRNAs into the P-site of the 40S subunit. In addition to its role in initiation, can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1.