Krt6a (NM_008476) Mouse Untagged Clone

CAT#: MC218808

Krt6a (untagged) - Mouse keratin 6A (Krt6a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Krt6a
• Synonyms: 2310016L08Rik; Krt2-6a; Krt2-6c; MK6a; mK6[a]
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218808 representing NM_008476
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTACCAAAACCACCATCAAAAGTCAAACCAGCCACCGTGGCTACAGTGCCAGCTCAGCCAGAGTGC
  CTGGGCTCAACCGCTCTGGCTTCAGCAGTGTGTCCGTGTGCCGCTCCCGGGGCAGCGGTGGCTCCAGTGC
  AATGTGTGGAGGAGCTGGCTTTGGCAGCAGGAGCCTCTATGGTGTGGGGAGCTCCAAGAGGATCTCCATC
  GGAGGGGGCAGCTGTGGCATTGGAGGAGGCTATGGCAGCCGATTTGGAGGAAGCTTCGGCATTGGAGGTG
  GAGCTGGTAGTGGCTTTGGCTTCGGTGGTGGAGCTGGCTTTGGTGGTGGCTATGGGGGAGCTGGCTTCCC
  GGTGTGCCCACCTGGAGGCATCCAAGAGGTCACCATCAACCAGAGCCTCCTCACACCCCTGAACCTGCAA
  ATTGACCCCACCATCCAGCGGGTCAGGACTGAGGAGAGGGAGCAGATCAAGACCCTCAACAACAAGTTTG
  CCTCCTTCATCGACAAGGTGCGGTTCCTGGAGCAGCAGAACAAGGTCCTGGACACCAAGTGGGCCCTGCT
  GCAAGAGCAGGGCACCAAGACCGTGAGGCAGAACCTGGAGCCTATGTTTGAGCAGTACATCAGCAACCTC
  CGCAGACAGCTGGACAGCATCATTGGAGAGAGGGGTCGCCTGGACTCAGAGCTGAGGAACATGCAGGACA
  CAGTGGAGGACTACAAGAGCAAATATGAAGATGAAATCAACAAGCGCACAGCAGCAGAGAATGAATTCGT
  GACCCTGAAGAAGGATGTAGATGCTGCCTACATGAACAAAGTTGAACTGCAAGCCAAGGCAGACAGTCTA
  ACAGATGATATCAACTTCTTGAGAGCTCTCTATGAAGCAGAACTGTCTCAGATGCAAACTCACATCTCAG
  ACACATCTGTGGTCCTCTCCATGGACAACAACCGTAGCCTGGACCTGGACAGCATCATCGCTGAGGTCAA
  GGCCCAGTATGAGGACATTGCTCAGAGAAGTCGGGCTGAAGCTGAGTCCTGGTACCAGACTAAATATGAG
  GAGCTGCAGGTCACAGCTGGCAGACATGGGGACGACCTACGCAACACCAAGCAGGAGATTGCTGAGATCA
  ACCGCATGATCCAGAGGCTGAGATCTGAGATCGACCACGTTAAGAAGCAGTGTGCCAACCTGCAAGCTGC
  TATTGCTGATGCTGAGCAACGTGGGGAGATGGCCCTGAAGGATGCCAGGGGCAAGCTGGAAGGGCTGGAG
  GATGCCCTGCAGAAGGCCAAACAGGACATGGCCAGGCTGCTGAAGGAGTACCAGGAACTCATGAATGTCA
  AGCTGGCCCTGGATGTGGAAATTGCCACCTACAGGAAGCTGCTGGAAGGAGAGGAGTGCAGGTTGAATGG
  TGAAGGTGTTGGACCAGTCAACATCTCTGTGGTGCAGTCCACCGTGTCCAGCGGCTATGGCAGTGCCGGG
  GGTGCCAGCAGCAGCTTAGGCCTGGGTGGAGGCAGCAGCTACTCCTATAGCAGCAGCCATGGCCTTGGAG
  GTGGCTTCAGTGCTGGCAGTGGCAGAGCCATCGGAGGTGGCCTCAGCTCTTCTGGTGGCCTCAGCTCTTC
  TACCATCAAATACACCACCACCTCCTCCAGCAAGAAGAGCTACAGGCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008476
• Insert Size: 1662 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008476.3, NP_032502.3
• RefSeq Size: 2287 bp
• RefSeq ORF: 1662 bp
• Locus ID: 16687
• UniProt ID: P50446
• Cytogenetics: 15 56.97 cM
• Gene Summary: Epidermis-specific type I keratin involved in wound healing (PubMed:10866680). Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration (PubMed:10866680). Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair (PubMed:22529101).[UniProtKB/Swiss-Prot Function]