Fnbp1 (NM_001038700) Mouse Untagged Clone

CAT#: MC218803

Fnbp1 (untagged) - Mouse formin binding protein 1 (Fnbp1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Fnbp1
• Synonyms: 1110057E06Rik; 2210010H06Rik; FBP1; Fbp17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218803 representing NM_001038700
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTGGGGCACTGAGCTCTGGGATCAGTTTGACAACTTGGAAAAACATACACAGTGGGGAATCGATA
  TTCTTGAGAAATACATCAAGTTTGTCAAGGAGAGGACGGAGATTGAGCTCAGCTATGCCAAGCAACTCAG
  GAATCTTTCAAAGAAATACCAACCTAAGAAGAACTCGAAGGAAGAGGAGGAGTACAAGTACACGGCTTGC
  AAAGCCTTTCTTTCCACCCTGAATGAGATGAATGACTACGCCGGGCAGCACGAGGTCATCTCTGAGAACA
  TGACGTCACAGATCACGGTGGACCTGATGCGCTACGTTCAGGAGCTGAAGCAGGAGAGGAAATCGAACTT
  CCATGATGGACGGAAGGCTCAGCAGCACATAGAAACGTGTTGGAAGCAACTGGAGTCAAGTAAGAGGAGG
  TTTGAGCGGGACTGTAAGGAAGCCGACCGGGCACAGCAGTACTTCGAGAAAATGGACGCTGACATCAACG
  TGACCAAGGCGGATGTGGAAAAGGCACGACAACAAGCTCAGATACGCCAGCAAATGGCAGAGGACAGCAA
  AGCAGATTACTCCTTAATCCTGCAGAGATTCAACCAGGAGCAATGGGAATACTACCATACCCACATCCCC
  AACATCTTCCAGAAAATACAAGAGATGGAGGAGAGGCGGATTGTGCGTATTGGGGAGTCCATGAAGACGT
  ACGCAGAGGTGGACCGGCAGGTGATACCCATCATCGGGAAGTGCCTGGACGGGATAGTGAAGGCGGCCGA
  GTCTATCGACCAGAAAAACGACTCCCAGCTGGTCGTAGAAGCCTATAAGTCAGGATTCGAGCCTCCTGGA
  GACATTGAATTCGAAGATTACACACAGCCAATGAAACGCACAGTGTCAGACAACAGCCTTTCCAGCTCCA
  AAGAAGGCAAGCCTGAGCTCAGATTTGGCGGCAAGTCCAGAGGCAAGCTCTGGCCATTCATCAAGAAAAA
  CAAGGGTGTCACACCAGAAGACTTCAGCAACTTCCCACCTGAGCAGAGAAGGAAAAAACTACAACAGAAA
  GTTGACGATCTCAATAGAGAGATACAGAAGGAGACGGACCAGAGAGATGCCATCACCAAAATGAAAGATG
  TGTACCTAAAGAACCCTCAGATGGGAGACCCAGCCAGCCTGGACCAGAAGCTCACCGAAGTCACCCAGAA
  CATAGAGAAACTGCGGCTGGAGGCTCAGAAGTTTGAGGCCTGGCTGGCTGAGGTAGAAGGCAGACTCCCA
  GCTCGGAGTGAGCAGGCACGCCGGCAGAGTGGACTGTATGATGGCCAGACACACCAGACGGTCACTAACT
  GTGCCCAGGACCGGGAGAGCCCAGATGGTAGTTACACAGAGGAGCAAAGCCAGGAGAGCGAGCACAAGGT
  CCTGGCCCCGGATTTCGACGATGAATTTGATGATGAGGAGCCGCTTCCAGCCATAGGGACCTGCAAGGCC
  CTCTACACATTTGAAGGTCAGAACGAAGGCACCATTTCCGTAGTTGAAGGAGAGACGCTGAGCGTGATTG
  AAGAGGACAAAGGCGATGGGTGGACTCGCATCCGCAGAAATGAAGACGAGGAGGGTTACGTCCCCACTTC
  CTACGTCGAAGTCTATTTAGACAAAAACGCCAAAGGTTCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001038700
• Insert Size: 1653 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001038700.2, NP_001033789.1
• RefSeq Size: 4545 bp
• RefSeq ORF: 1653 bp
• Locus ID: 14269
• UniProt ID: Q80TY0
• Cytogenetics: 2 B
• Gene Summary: Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during the late stage of clathrin-mediated endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also enhances actin polymerization via the recruitment of WASL/N-WASP, which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. May act as a link between RND2 signaling and regulation of the actin cytoskeleton. May be required for the lysosomal retention of FASLG/FASL (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) lacks two alternate, in-frame exons, compared to variant 3. The resulting protein (isoform a) is shorter when it is compared to isoform c.