Foxc1 (NM_008592) Mouse Untagged Clone

CAT#: MC218787

Foxc1 (untagged) - Mouse forkhead box C1 (Foxc1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Foxc1
• Synonyms: ch; fkh-1; Fkh1; FREAC3; frkhda; Mf1; Mf4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218787 representing NM_008592
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGCGCGCTACTCGGTGTCCAGCCCCAACTCCCTGGGAGTGGTGCCCTACCTCGGCGGCGAGCAGA
  GCTACTATCGCGCTGCCGCGGCGGCGGCCGGGGGCGGCTACACCGCCATGCCGGCCCCTATGAGCGTGTA
  CTCGCACCCTGCTCACGCCGAGCAGTACCCGGGCAGCATGGCGCGCGCCTACGGGCCTTATACGCCGCAG
  CCGCAGCCCAAGGACATGGTGAAGCCGCCCTACAGCTACATCGCTCTTATCACCATGGCCATCCAGAATG
  CCCCGGACAAGAAGATCACTCTGAATGGCATCTACCAGTTCATCATGGACCGCTTCCCCTTCTATCGGGA
  CAATAAGCAGGGCTGGCAGAACAGCATACGGCACAACCTCTCGCTCAACGAGTGCTTCGTCAAGGTGCCC
  CGCGACGACAAGAAGCCAGGCAAGGGCAGCTACTGGACGCTCGACCCGGACTCCTACAACATGTTCGAGA
  ACGGCAGCTTCCTGCGGCGGCGGCGGCGCTTCAAGAAGAAGGACGCAGTGAAGGACAAGGAGGAGAAGGG
  CCGGCTGCACCTCCAAGAACCGCCACCGCCGCAGGCCGGCCGCCAGCCCGCGCCCGCGCCCCCGGAGCAG
  GCCGAGGGCTCCGCTCCCGGGCCACAGCCGCCGCCCGTGCGCATCCAGGACATCAAGACGGAGAACGGTA
  CGTGTCCCTCGCCTCCCCAGCCCCTGTCCCCGGCTGCCGCCCTAGGCAGCGGCAGCGCCGCCACAGTGCC
  CAAAATCGAGAGCCCCGACAGCAGCAGCAGCAGCTTGTCGAGCGGGAGCAGCCCCCCGGGCAGCCTGCCG
  TCGGCGCGGCCGCTCAGCCTGGACGCTGCAGAACCCGCGCCGCCGCCACAGCCTGCGCCGCCGCCGCATC
  ACAGCCAGGGCTTCAGCGTGGACAACATCATGACGTCGCTGCGGGGGTCGCCGCAGGGTTCGGCCGCTGA
  GCTCGGTTCCGGCCTCCTGGCCTCGGCGGCTGCGTCCTCGCGCGCGGGCATCGCGCCCCCGCTGGCGCTG
  GGTGCCTACTCTCCGGGCCAGAGCTCCCTCTACAGCTCCCCCTGCAGCCAGAGCTCCAGTGCGGGCAGTT
  CGGGCGGCGGGGGTGGCGGCGGCGGCGGAGGCGGCGGCAGCAGCAGCGCTGCGGGTACGGGGGGCGCCGC
  CACTTACCACTGCAACCTGCAGGCTATGAGCCTGTACGCGGCGGGCGAGCGTGGCGGCCACTTGCAGGGT
  CCGGCGGGAGGCGCGGGCAGCGCGGCGGTGGACGACCCCCTGCCCGACTACTCGCTGCCTCCAGCGACCA
  GCAGCAGCTCTTCGTCCCTGAGTCATGGCGGGGGCGGCCAGGAGGCCAGCCACCACCCTGCATCCCACCA
  GGGCCGACTCACCTCGTGGTACCTGAACCAGGCAGGTGGAGACCTGGGCCACTTGGCGAGCGCGGCGGCG
  GCTGCGGCGGCCGCAGGCTACCCTGGCCAGCAGCAGAACTTCCACTCGGTGCGGGAAATGTTCGAGTCTC
  AGCGGATCGGCTTGAACAACTCCCCGGTGAATGGGAATAGTAGCTGTCAGATGGCTTTCCCTGCCAGTCA
  GTCTCTGTACCGCACGTCGGGGGCTTTCGTCTATGACTGTAGCAAATTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008592
• Insert Size: 1662 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008592.2, NP_032618.2
• RefSeq Size: 3990 bp
• RefSeq ORF: 1662 bp
• Locus ID: 17300
• UniProt ID: Q61572
• Cytogenetics: 13 13.52 cM
• Gene Summary: DNA-binding transcriptional factor that plays a role in a broad range of cellular and developmental processes such as eye, bones, cardiovascular, kidney and skin development (PubMed:9635428, PubMed:9106663, PubMed:10479458, PubMed:10395790, PubMed:11562355, PubMed:18187037, PubMed:19668217, PubMed:22493429, PubMed:24590069, PubMed:25808752, PubMed:28223138). Acts either as a transcriptional activator or repressor (PubMed:28223138). Binds to the consensus binding site 5'-[G/C][A/T]AAA[T/C]AA[A/C]-3' in promoter of target genes (PubMed:25808752). Upon DNA-binding, promotes DNA bending. Acts as a transcriptional coactivator (PubMed:25808752). Stimulates Indian hedgehog (Ihh)-induced target gene expression mediated by the transcription factor GLI2, and hence regulates endochondral ossification (PubMed:25808752). Acts also as a transcriptional coregulator by increasing DNA-binding capacity of GLI2 in breast cancer cells. Regulates FOXO1 through binding to a conserved element, 5'-GTAAACAAA-3' in its promoter region, implicating FOXC1 as an important regulator of cell viability and resistance to oxidative stress in the eye (By similarity). Cooperates with transcription factor FOXC2 in regulating expression of genes that maintain podocyte integrity (PubMed:28223138). Promotes cell growth inhibition by stopping the cell cycle in the G1 phase through TGFB1-mediated signals. Involved in epithelial-mesenchymal transition (EMT) induction by increasing cell proliferation, migration and invasion (By similarity). Involved in chemokine CXCL12-induced endothelial cell migration through the control of CXCR4 expression (PubMed:18187037). Plays a role in the gene regulatory network essential for epidermal keratinocyte terminal differentiation (By similarity). Essential developmental transcriptional factor required for mesoderm-derived tissues formation, such as the somites, skin, bone and cartilage (PubMed:9106663, PubMed:10479458, PubMed:10395790, PubMed:10704385, PubMed:11562355, PubMed:15196959). Positively regulates CXCL12 and stem cell factor expression in bone marrow mesenchymal progenitor cells, and hence plays a role in the development and maintenance of mesenchymal niches for haematopoietic stem and progenitor cells (HSPC) (PubMed:24590069). Plays a role in corneal transparency by preventing both blood vessel and lymphatic vessel growth during embryonic development in a VEGF-dependent manner (PubMed:22171010). May function as a tumor suppressor (By similarity).[UniProtKB/Swiss-Prot Function]