Det1 (NM_029585) Mouse Untagged Clone

CAT#: MC218781

Det1 (untagged) - Mouse de-etiolated homolog 1 (Arabidopsis) (Det1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Det1
• Synonyms: 2610034H20Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218781 representing NM_029585
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCATCATGTTTCCACAATTAAACCTCGAAGAATCCAAAATCAAAATGTCATTCACCGCCTCGAAC
  GCCGGCGCATCAGTTCAGGCAAGGCGGGCACCCACTGGCATCAGGTCCGAGTGTTCCACCAGAACGTCTT
  CCCCAACTTCACGGTTGTCAACGTCGAAAAGCCTCCTTGTTTCTTACGGAAATTCTCGCCTGACGGGCGC
  TACTTTATTGCCTTTTCATCTGACCAGACATCTCTTGAAATCTATGAGTACCAGGGCTGTCAGGCAGCAG
  AGGACCTCTTGCAGGGATACGAAGGGGAGATCTTGTCCAATGGCAATGACCAGAGGTCGGTCAGCATCCG
  AGGTCGGCTCTTTGAGCGTTTTTTTGTTCTGCTGCACATTACCAACGTGGCGGCCAATGGCGAGCACTTG
  AACCGGGAGTGTAGCCTCTTCACAGATGACTGCCGCTGCGTCATCGTGGGCTCTGCTGCCTACCTCCCAG
  ACGAGCCTCACCCTCCATTCTATGAGGTGTATCGGAACAGTGAGTCGGTGACCCCCAATCCACGGTCCCC
  ACTTGAGGACTATTCCCTCCACATCATTGACCTTCACACTGGCCGTTTGTGTGACACACGCACATTCAAG
  TGTGACAAAGTGGTCTTGTCGCACAATCAAGGGCTCTACTTGTATAAAAACATCCTGGCCATATTGTCTG
  TGCAGCAGCAGACGATTCATGTCTTCCAGGTGACCCCTGAAGGCACCTTTATCGACGTTCGAACCATCGG
  CCGCTTCTGCTATGAGGATGACCTGCTCACTGTGTCTGCCGTTTTCCCTGAGGTGCAGCGGGACAGCCAG
  ACAGGCATGGCCAACCCTTTCAGGGACCCTTTCATCAACTCCTTGAAGCACCGCTTGCTGGTTTACCTCT
  GGCGCCGGGCAGAGCAGGATGGCAGTGCCATGGCGAAGAGGCGGTTCTTCCAGTACTTTGACCAGCTGCG
  CCAGCTGCGCATGTGGAAGATGCAGCTCCTGGACGAGAACCATCTGTTCATCAAGTACACCAGCGAGGAC
  GTAGTGACGCTGCGGGTCACAGACCCGTCTCAGGCCTCCTTCTTTGTGGTATACAACATGGTGACAACAG
  AGGTGATTGCCGTGTTTGAGAACACATCCGATGAGCTTCTGGAACTCTTCGAGAACTTCTGTGACCTCTT
  CCGCAACGCCACCCTGCACAGTGAAGTCCAATTCCCTTGCTCAGCTTCTAGCAACAACTTTGCAAGGCAG
  ATCCAGCGCCGGTTCAAAGACACGATTATAAATGCCAAGTATGGAGGGCACACGGAGGCTGTGCGCCGAC
  TGCTAGGCCAGCTCCCCATCAGTGCCCAGTCTTACAGTGGGAGCCCATACCTGGATTTGTCTCTCTTCAG
  CTACGATGACAAGTGGGTGTCAGTCATGGAGCGACCCAAGACTTGTGGAGATCACCCGATCAGGTTCTAT
  GCCCGGGACTCTGGCTTGCTGAAGTTTGAGATCCAGGCGGGCTTGCTGGGCCGCCCCATCAACCACACAG
  TGCGACGCCTCGTGGCCTTCACCTTTCACCCCTTTGAGCCATTTGCCATTTCTGTGCAGAGGACTAACGC
  AGAGTATGTTGTCAACTTCCACATGCGACACTGCTGCACCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029585
• Insert Size: 1653 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029585.3, NP_083861.1
• RefSeq Size: 2217 bp
• RefSeq ORF: 1653 bp
• Locus ID: 76375
• UniProt ID: Q9D0A0
• Cytogenetics: 7 D2
• Gene Summary: Component of the E3 ubiquitin ligase DCX DET1-COP1 complex, which is required for ubiquitination and subsequent degradation of target proteins. The complex is involved in JUN ubiquitination and degradation (By similarity).[UniProtKB/Swiss-Prot Function]