Gabra4 (NM_010251) Mouse Untagged Clone

CAT#: MC218772

Gabra4 (untagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit alpha 4 (Gabra4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gabra4
• Synonyms: Gabra-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218772 representing NM_010251
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTCTGTCCAGAAGGTACCCGCGATTGCGCTGTGCTCCGGGGTCAGCCTCGCCCTCCTGCACTTCC
  TGTGCCTGGCGGCTTGTTTAAACGAATCCCCAGGACAGAACTCAAAGGACGAGAAATTGTGCCCGGAAAA
  TTTTACCCGTATTCTGGACAGTTTGCTGGATGGTTATGACAACAGGCTGCGTCCTGGATTTGGGGGTCCT
  GTTACAGAAGTGAAAACTGATATATATGTCACCAGCTTTGGGCCCGTTTCTGATGTTGAAATGGAATACA
  CTATGGATGTGTTCTTCAGACAGACATGGATTGACAAAAGACTAAAATATGACGGCCCAATTGAAATCTT
  GAGGCTGAATAATATGATGGTCACCAAAGTTTGGACCCCTGATACTTTCTTCAGGAATGGAAAGAAATCT
  GTCTCACATAACATGACAGCTCCAAATAAGCTTTTTAGAATTATGAGAAATGGCACTATTTTATACACAA
  TGAGACTCACCATAAGTGCGGAGTGCCCCATGAGACTGGTGGATTTTCCTATGGATGGTCATGCCTGCCC
  TTTGAAATTTGGGAGTTATGCATATCCCAAAAGTGAGATGATCTACACCTGGACCAAAGGCCCTGAGAAG
  TCAGTGGAGGTGCCAAAGGAGTCTTCTAGCTTAGTTCAATATGACCTCATTGGGCAGACTGTATCAAGCG
  AGACTATCAAATCTATTACAGGTGAATACATTGTTATGACGGTTTACTTCCACCTCAGACGGAAGATGGG
  CTACTTTATGATTCAGACGTATATCCCATGCATCATGACAGTGATTCTTTCTCAAGTTTCCTTCTGGATA
  AACAAGGAGTCTGTTCCAGCTAGAACTGTATTTGGAATAACCACAGTCCTCACGATGACCACCCTAAGCA
  TCAGTGCTCGGCATTCTTTGCCCAAAGTGTCCTATGCGACTGCCATGGATTGGTTCATAGCTGTCTGTTT
  TGCTTTTGTATTTTCGGCTCTTATTGAGTTTGCTGCTGTCAACTATTTCACCAACATTCAAATGCAGAAA
  GCCAAAAAGAAGATATCAAAGCCTCCCCCAGAAGTTCCAGCTGCTCCTGTGCTGAAGGAGAAACACACAG
  AAACATCCCTTCAGAATACACATGCCAATTTGAACATGAGGAAAAGAACAAATGCCTTGGTCCATTCAGA
  ATCGGATGTCAAAAGCAGAACTGAGGTGGGAAATCACTCCAGCAAGACCAGCGCTGTCCAGGAGTCTTCT
  GAAGCCACGCCTAAGGCTCACTTAGCTTCCAGTCCAAATCCATTCAGCAGGGCAAATGCAGCTGAGACTA
  TGTCTGCTGCAGCCAGAGGTCTTTCATCTGCAGCATCCCCCTCTCCTCATGGCACATTGCGGCCAGCTTC
  TTTGGGGTCAGCTTCCACTCGCCCTGCATTTGGATCTAGACTTGGGCGAATTAAGACAACAGTTAATACA
  ACAGGGGCTGCTGGGAATGTGTCAGCCACACCTCCTCCCCCTGCTCCACCGCCTTCTGGATCTGGCACAA
  GTAAAATAGACAAATATGCTCGTATTCTCTTTCCAGTCACATTTGGAGCATTTAACATGGTCTACTGGGT
  TGTTTATTTATCTAAGGACACCATGGAGAAATCAGAAAGTCTAATGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010251
• Insert Size: 1659 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010251.2, NP_034381.1
• RefSeq Size: 4123 bp
• RefSeq ORF: 1659 bp
• Locus ID: 14397
• UniProt ID: Q9D6F4
• Cytogenetics: 5 C3.2
• Gene Summary: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.[UniProtKB/Swiss-Prot Function]