Erlin1 (NM_145502) Mouse Untagged Clone

CAT#: MC218744

Erlin1 (untagged) - Mouse ER lipid raft associated 1 (Erlin1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Erlin1
• Synonyms: 2810439N09Rik; C80197; Keo4; Spfh1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218744 representing NM_145502
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATATGACTCAAGCCCGGCTTCTGGTGGCTGCAGTGGTGGGGTTGGTGGCGATCCTCCTGTACGCCT
  CCATCCACAAGATCGAAGAGGGACACTTGGCCGTGTACTACAGGGGAGGAGCTTTGCTAACGAGCCCCAG
  TGGACCAGGCTATCATATCATGTTGCCTTTCATTACAACATTCAGATCTGTGCAGACAACACTACAAACG
  GATGAAGTTAAAAATGTGCCTTGTGGAACAAGTGGTGGAGTCATGATCTATATTGACCGAATAGAAGTGG
  TTAATATGTTGGCTCCTTATGCAGTGTTTGACATTGTGAGGAACTATACTGCAGACTACGACAAGACTTT
  AATCTTCAATAAAATCCACCATGAGCTGAACCAGTTTTGCAGTGCCCACACACTTCAAGAAGTTTACATA
  GAATTGTTTGATCAAATAGATGAAAACCTGAAGCAGGCCCTGCAAAAAGATTTAAACACCATGGCCCCAG
  GTCTCACTATCCAGGCTGTGCGTGTTACAAAACCCAAAATCCCAGAAGCCATAAGAAGAAATTTTGAATT
  AATGGAGGCAGAGAAGACAAAACTTCTCATAGCTGCACAGAAACAAAAGGTGGTGGAGAAAGAAGCTGAG
  ACGGAGAGGAAAAGGGCTGTTATAGAAGCAGAGAAGATTGCACAAGTAGCAAAAATTCGATTTCAACAGA
  AAGTGATGGAGAAAGAAACTGAAAAACGCATTTCTGAGATTGAAGATGCTGCGTTCCTGGCCCGAGAGAA
  GGCAAAAGCAGATGCCGAGTATTACGCTGCACACAAATACGCCACCTCAAACAAGCACAAACTGACCCCA
  GAGTATCTGGAGCTCAAGAAATACCAGGCCATTGCCTCAAACAGTAAGATCTACTTTGGCAGCAACATCC
  CCAGCATGTTTGTGGACTCCTCCTGTGCTCTGAAATACTCTGATGGTAGGACTGGGAGAGAAGACTCCCT
  TCCCCCAGAGGAGGCCCGTGAGCCCTCTGGAGAGAGCCCCATCCAAAACAAGGAGAACGCAGGTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145502
• Insert Size: 1047 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145502.3, NP_663477.3
• RefSeq Size: 3349 bp
• RefSeq ORF: 1047 bp
• Locus ID: 226144
• UniProt ID: Q91X78
• Cytogenetics: 19 C3
• Gene Summary: Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Involved in regulation of cellular cholesterol homeostasis by regulation the SREBP signaling pathway. Binds cholesterol and may promote ER retention of the SCAP-SREBF complex (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript. All three variants encode the same protein.