Agxt2 (NM_001031851) Mouse Untagged Clone

CAT#: MC218663

Agxt2 (untagged) - Mouse alanine-glyoxylate aminotransferase 2 (Agxt2), nuclear gene encoding mitochondrial protein, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Agxt2
• Synonyms: AI303810; AI663818
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC218663 representing NM_001031851
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCTAGCCTGGAGAAATTTGCAGAAACCCTTTTATCTGGAGACTTCTCTCAGAATACTCCAGATGC
  GTCCATCCCTGAGCTTAGGTGCTTCCCGGATAGCTGTGCCCAAGCTCACTCTTCATACAAAGCACAGCAT
  GCCACCGTGTGACTTCTCACCTGAGAAATACCAGTCCCTGGCCTACAGCCGTGTGCTGGCGATTCACAAG
  CAGCATCTTTCTCCTGTGGATACAGCCTATTTCCGGAAGCCTTTGCTACTCCACCAGGGACACATGGAGT
  GGCTCTTTGATTCTGAGGGAAACAGATACCTGGACTTCTTCTCTGGGATTGTCACTGTTAGTGTTGGTCA
  CTGCCACCCGAAGGTAAGTGCAGTGGCAAAAAAACAGATAGACCGCCTGTGGCATACAAGTTCTGTCTTC
  TTCCACTCTCCGATGCATGAATATGCAGAGAAGCTCTCAGCACTCCTTCCTGAGCCTCTTAAGGTCATTT
  TCTTGGTGAACAGTGGATCAGAAGCCAACGACCTGGCCATGGTGATGGCCCGAGCACACTCGAATCACAC
  AGACATCATATCTTTCAGAGGAGCCTATCACGGATGCAGTCCCTATACACTTGGCCTGACAAATGTCGGA
  ATCTACAAGATGGAAGTCCCTGGTGGGATAGGCTGCCAATCAACAATGTGCCCAGATGTTTTCCGTGGCC
  CTTGGGGAGGAATCCATTGTCGAGATTCTCCAGTGCAAACAGTGAGGGACTGCAGCTGTGCTCCAGGTCC
  AAATGGGCAGGGAGGAAGGCGAGAATGCCATTCTAACATCAAATGCTGTGACTTGTGTATGTGTGTATGT
  TTTGCTACAGACTGCTGCCAAGCTAAAGAACGGTATATCGAACAATTCAAAGACACACTGAACACTTCTG
  TGGCCACATCAATTGCTGGATTTTTTGCAGAGCCAATTCAAGGTGTGAATGGTGTTGTCCAGTACCCAAA
  GGAGTTTCTGAAGGAAGCCTTTGCGTTGGTCCGAGAGCGGGGAGGTGTGTGCATCGCAGATGAAGTGCAG
  ACAGGCTTTGGCAGGCTGGGCTCTCACTTCTGGGGTTTCCAGACACATGATGTACTTCCTGACATTGTCA
  CCATGGCTAAAGGGATTGGAAATGGCTTCCCCATGGCTGCAGTTGTGACCACTCCAGAAATTGCGAAGTC
  TTTGGCTAAACGTTTGCTTCACTTCAGCACATTTGGAGGAAATCCCCTGGCCTGCGCCATTGGATCTGCT
  GTGCTTGAGGTGATTGAAGAAGAAAATCTCCAGAGAAACAGTCAAGAAGTTGGCACCTACATGCTGCTGA
  AGTTTGCTAAGCTTCGGGACGAATTTGATATCGTTGGGGATGTCCGAGGCAAAGGCCTTATGGTGGGGAT
  AGAGATGGTTCAAGACAAGATAAGCCGACAACCTCTTCCTAAAACAGAAGTCAATCAAATCCATGAGGAC
  TGCAAAGACATGGGGCTCCTAGTGGGTAGAGGTGGTAATTTTTCTCAGACTTTTCGAATTGTGCCCCCGA
  TGTGTGTCACGAAGATGGAAGTAGATTTTGCATATGAAGTATTTCGAGCTGCCTTAATTCAACACATGGA
  GAGAAGAGCTAAGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001031851
• Insert Size: 1626 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001031851.1, NP_001027021.1
• RefSeq Size: 1825 bp
• RefSeq ORF: 1626 bp
• Locus ID: 268782
• Cytogenetics: 15 A1
• Gene Summary: Can metabolize asymmetric dimethylarginine (ADMA) via transamination to alpha-keto-delta-(NN-dimethylguanidino) valeric acid (DMGV). ADMA is a potent inhibitor of nitric-oxide (NO) synthase, and this activity provides mechanism through which the kidney regulates blood pressure.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses alternate, in-frame donor and acceptor splice sites in the mid-coding region compared to variant 1. The resulting isoform (3) is longer containing an internal protein segment not found in isoform 1.